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PCR method for identifying agkistrodon and specific primer thereof

A technology of specific primers and identification methods, which is applied in the field of identification of traditional Chinese medicine and Chinese medicinal materials, and achieves the effect of shortening the extraction time

Inactive Publication Date: 2009-12-30
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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Before the present invention was published, there was no disclosure or report of the PCR primers and methods mentioned in this patent application

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  • PCR method for identifying agkistrodon and specific primer thereof
  • PCR method for identifying agkistrodon and specific primer thereof
  • PCR method for identifying agkistrodon and specific primer thereof

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Embodiment Construction

[0017] 1 material

[0018] Viper samples came from Hebei Anguo area, Beijing Tongrentang Pharmacy, Beijing Jinxiang Pharmacy, Beijing Sunshine Tongren Pharmacy, Beijing Jinglongtang Pharmacy, etc. See Table 1 for details. The samples were all identified by Director Zhang Ji of the Herbarium of Chinese Medicine Department of China Institute for the Control of Pharmaceutical and Biological Products.

[0019] Table 1 Sample source table

[0020]

[0021]

[0022] 2 Design of specific primers

[0023] Search for the cytochrome b (cytb) sequences of more than 20 species of snakes such as Viper and its common confounds in GENBANK, such as Elaphe japonica, Elaphe japonica, mouse snake, and cobra, and use DNAMAN to perform homologous comparison. Design a pair of specific primers P1, P2, the sequence is as follows:

[0024] P1: 5'-GGCAATTCACTACACACAGCCAACATCAACT-3'

[0025] P2: 5'-CCATAGTCAGGTGGTTAGTGATAC-3'

[0026] 3DNA extraction

[0027] The DNA of various materials was...

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Abstract

The invention belongs to the technical field of Chinese medicaments and the identification on Chinese medicinal materials, and particularly relates to a PCR method capable of specifically identifying agkistrodon and a specific primer thereof. When the method is used to identify the true or false of the agkistrodon, the identification on the true or false of a sample can be finished through simple DNA extraction, PCR specific amplification and electrophoresis detection. The method has the advantages of simple operation, strong specificity, good repetitiveness and the like. The method is mainly used for the rapid identification of agkistrodon medicinal materials, preparations containing the agkistrodon and agkistrodon living materials.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicines and medicinal materials, in particular to a PCR method capable of specifically identifying Vipers and specific primers thereof. It is mainly used for rapid identification of Viper medicinal materials, preparations containing Viper and living materials of Viper. Background technique [0002] Agkistrodon acutus Güenther is a commonly used traditional Chinese medicine recorded in the Chinese Pharmacopoeia (2005 edition), which is derived from the dried body of Agkistrodon acutus Güenther, a Viperidae animal. It has the effects of expelling wind, dredging collaterals and relieving spasm. For rheumatic obstinate numbness, numbness and spasm, hemiplegia, convulsions, tetanus, leprosy and scabies (Chinese Pharmacopoeia. One. 2005.258)). [0003] The clinical efficacy of Viper as a precious animal medicinal material is definite, but the commodity source is complex...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 黄璐琦崔光红赵静雪唐晓晶
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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