Autoimmune regulation of prostate cancer by annexin a3

A technology of prostate cancer and annexin, which is applied in the field of diagnosis of prostate cancer, can solve the problems of little knowledge about biology

Inactive Publication Date: 2010-03-10
PROTEOSYS AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, little is known about the role and biology of ANXA3 in the human prostate

Method used

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  • Autoimmune regulation of prostate cancer by annexin a3
  • Autoimmune regulation of prostate cancer by annexin a3
  • Autoimmune regulation of prostate cancer by annexin a3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0054] I. Materials and methods

[0055] 1.1 Antibody

[0056] The following antibodies were used in this study:

[0057] Clone tgc7 ProVII5C5 (DSM ACC2780), monoclonal, 2.4 mg / ml; against the target protein ANXA3.

[0058] 1.2 Organization

[0059] Existing prostate cancer TMAs containing >3000 tissue samples were analyzed. A detailed description of the composition is provided in the Results section.

[0060] 1.3 Immunohistochemistry (IHC)

[0061] An IHC protocol suitable for Annexin A3 detection in formalin-fixed tissues was developed. The detailed scheme is given below.

[0062] ANXA3 Immunostaining (IHC) Protocol

[0063] slide preparation

[0064] • Dewax TMAs in xylene for at least 1 hour, GFS 2 x 5 minutes in xylene.

[0065] ● Rehydration in descending ethanol series up to distilled water

[0066] ●Rinse in PBS buffer for 5 minutes

[0067] Pretreatment (epitope recovery)

[0068] ● Autoclave slides in acetic acid pH 2 for 5 minutes

[0069] ●Rinse in PBS...

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Abstract

The present invention relates to a method for the diagnosis of prostate carcinoma comprising the determination of annexin A3 (ANXA3) and / or autoantibodies against ANXA3 with a specific reagent. The method allows differentiation between benign, premalignant and malignant conditions. Further, the method has a prognostic relevance.

Description

technical field [0001] The present invention relates to a method for diagnosing prostate cancer, said method comprising measuring annexin A3 (ANXA3) and / or autoantibodies against ANXA3 with specific reagents. The present invention allows the distinction between benign, precancerous and malignant conditions. Furthermore, the method has prognostic relevance. Background technique [0002] ANXA3 was shown to be significantly associated with prostate cancer in a previous differential quantitative protein expression study (1). However, little is known about the role and biology of ANXA3 in the human prostate. ANXA3 has been shown to be part of so-called exosomes (small vesicles released by various types of epithelial cells) (2), whose role in the regulation of the innate immune system is poorly understood (3), and they The anti-tumor activity of β was discovered very recently (4). Recent reports have shown autoimmunity in prostate cancer patients against so-called prostatic bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N2333/4718G01N33/574C07K16/32
Inventor A·施拉滕赫尔兹
Owner PROTEOSYS AG
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