Method for preparing biodiesel from germinated oil crop seeds serving as raw material through autocatalysis

Abstract

The invention relates to a method for preparing biodiesel from germinated oil crop seeds serving as a raw material through autocatalysis. The method comprises the following steps of: soaking oil crop seeds in clear water for 12 hours, and performing germination culture on the soaked oil crop seeds in the dark at the temperature of between 15 and 35 DEG C; performing freeze drying on the germinated oil crop seeds at the temperature of between -50 and -20 DGE C for 12 to 48 hours and then crushing the freeze-dried oil crop seeds; adding an organic solvent and short-chain alcohol into the germinated oil seed powder in turn, wherein 1 to 15ml of the organic solvent is added into every gram of the germinated oil seed powder, and the short-chain alcohol accounts for 2 to 10 percent of the mass of the germinated oil seed powder; and putting the mixture in a temperature-controlled reciprocating water bath shaker and performing esterification and ester exchange reaction for 4 to 24 hours to finally obtain the biodiesel, wherein the yield of the biodiesel is between 40 and 70 percent. In the method, a catalyst is not needed, the organic solvent is used as an oil extracting agent for oil seeds and as a protective agent of lipase, and the oil extraction process and the esterification process are combined as one process, so that cost input from oil extraction and refining is eliminated.

Description

Technical field: The invention relates to the technical field of biodiesel preparation. Specifically, it is a method for preparing biodiesel by self-catalysis using germinated oil crop seeds as raw materials. Background technique: With the development of industry and economy, the demand for mineral energy such as petroleum and coal is increasing, and the depletion of petroleum resources and the improvement of people's awareness of environmental protection have greatly promoted the development of diesel alternative fuels in countries around the world. Especially in the 1990s, biodiesel, as an environmentally friendly, low-CO 2 Emission and non-toxic green renewable energy has attracted the attention of various countries. Therefore, vigorously developing diesel alternative energy biodiesel has particularly important strategic significance. The production methods of biodiesel include chemical methods and biocatalytic methods. The methods commonly used for large-scale indust...

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Problems solved by technology

The purpose of the present invention is to solve the problem that the biocatalyst immobilized enzyme in the existing biodiesel preparation process is expensive, the cost of the raw material substrate oil is relatively high, and the substrate short-chain alcohol has a toxic effect on the lipase, thereby making the lipase Due to defects such as loss of activity and stability, the present invention provides a method for preparing biodiesel by self-catalysis from germinated oil crop seeds. The method does not require additional catalysts, is simple in process, and is environmentally friendly, greatly reducing production costs and adapting to industrialization. production needs

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