Automated cell density adjustment method for producing analysis plate

A technology for analyzing plates and cells, which is applied in the field of devices for preparing cell analysis plates, and can solve the problems of no automatic operation, etc.

Inactive Publication Date: 2010-09-01
NOVACYT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Finally, if the amount of cell suspension could be adjusted directly when sampling in vials, an assay plate with the appropriate cell density could be obtained immediately, but there is no open fully automated operation for this process, nor any follow-up Quality operations can give reports of low cell densities in solutions that are set out initially in vials and can be corrected for by secondary enrichment on assay plates

Method used

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  • Automated cell density adjustment method for producing analysis plate
  • Automated cell density adjustment method for producing analysis plate
  • Automated cell density adjustment method for producing analysis plate

Examples

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Embodiment Construction

[0028] Said equipment generally comprises at least one cell sample bottle (not shown), at least one decanting chamber 2, said decanting chamber is arranged on the top of analysis plate 4, is used for sampling cell solution from sample bottle and decanting said cell The solution is introduced into a device (not shown) in the decanting chamber 2 .

[0029] The sample bottles and the decanting chamber 2 are arranged on a plate 6 equipped with at least one housing for receiving an analysis plate 4 located below the decanting chamber 2 . In practice, a plurality of decanting chambers 2 and analysis plates 4 fit in corresponding notches on a plate 6 used to build a series of analysis plates.

[0030] The decanting chamber 2 is a bottomless container so that the substances introduced into the decanting chamber can be poured onto the analysis plate, as described, for example, in the document FR-279333. The assay plate 4 is a conventional glass slide for this type of application.

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Abstract

This method comprises the following steps: - taking a sample of a cell solution from a sample bottle, - placing said sampled solution in a settling chamber (2) placed above the analysis plate (4), - leaving the solution to settle out in order to obtain cell spreading on the analysis plate (4). It comprises a step of measuring the cell density of the sampled solution, said measurement being carried out in the settling chamber (2). The invention also relates to a device for producing a cell analysis plate which makes it possible to implement such a method.

Description

technical field [0001] The present invention relates to a preparation method of a cell analysis plate (cell analysis plate), the type of the cell analysis plate includes a thin layer of cells to be detected or a cell dispersion (cell dispersion) arranged on the analysis plate, the method Include the following steps: [0002] - Take a sample of the cell solution from the vial; [0003] - placing the sampled solution in a decantation chamber arranged above the assay plate; [0004] - Slowly decant the solution to obtain a thin layer of the cells on the assay plate. The invention also relates to a device for the preparation of a cell analysis plate so that the method described above can be carried out. Background technique [0005] For example, cell assay plates are prepared for the determination of medical diagnoses, for which purpose cell sedimentation is achieved on glass slides. [0006] Take a cell sample with a sample brush, for example, in the case of a smear, place ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N15/06G01N33/48
CPCG01N1/2813G01N2001/282G01N2001/2826
Inventor E·佩尔蒂埃
Owner NOVACYT
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