50 results about "Cell spreading" patented technology

The present invention provides fully human antibodies that bind to respiratory syncytial virus F protein, compositions comprising the antibodies and methods of use. The antibodies of the invention are useful for preventing fusion of the virus with the cell membrane and preventing cell to cell spread of the virus, thereby providing a means of preventing the infection, or treating a patient suffering from the infection and ameliorating one or more symptoms or complications associated with the viral infection. The antibodies may also be useful for diagnosis of an infection by RSV.

The embodiments herein disclose a method of fabricating composite scaffolds for skin tissue regeneration. The methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA) are synthesized. The poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are synthesized. The hydrogel is synthesized. The composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds is fabricated. A plurality of physico-chemical characteristics of the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are analysed. The physico-chemical characteristics comprises mechanical properties, swelling ratio and enzymatic degradation and scanning electron microscope imaging. The fibroblast cells are encapsulated within the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds and hydrogels. The fibroblast cells are seeded on composite scaffold and PGS-PCL scaffold. The fibroblast cell viability, fibroblast cell attachment, fibroblast cell spreading, fibroblast cell proliferation and fibroblast cell metabolism are analysed in composite scaffolds, PGS-PCL scaffolds and hydrogels.

The invention discloses a medicament injection, which particularly relates to an injection which contains sea-buckthorn seed oil fat emulsion and a process for preparation. The sea-buckthorn seed oil fat emulsion of the invention is prepared according to the following components and proposition, 5 to 30g sea-buckthorn seed oil for injecting in each 100ml injection, 0.5 to 7.5g emulsifier, 1.5 to 3.6g isotonic regulator, 0.001 to 0.2g anti-oxidant, hydrochloric acid solution or sodium hydrate solution whose PH value is adjusted from 6.5 to 9.5, and the other are water for injecting. The proportions of the sea-buckthorn seed oil fat emulsion injection w-6 and w-3 are proper, and the stability is good, and the invention can not only provide heat energy for human bodies to replenish essential fatty acid for human bodies, but also the invention has the functions of improving body immunity, prompting tissue repair, anti-inflammatory anti-radiation and anti-mutation, and inhibiting cancer cell to diffuse. The invention particularly has a promoting effect to physical rapid restoration of postoperative patients, patients with large areas of burn, and cancer patients who have radiotheraphy and chemotherapy.

The invention discloses cancer diffusion transfer therapeutic equipment which ingrates a targeted function restoration therapy with a therapy chemoradiotherapy and prevents and cures the diffusion transfer of a cancer. The cancer diffusion transfer therapeutic equipment is advanced therapeutic technology integrally formed by a direct current low-medium frequency multifunctional rubbing head, lasers, ultrasonic waves, microwaves, radio frequencies, medicine external application injection intervention in a symmetrically combined mode, and is used for conducting a therapy of function restoration of a diseased region and prevention of diffusion transfer of cancer cells through the symmetrical combination of medicine massage, electrical stimulation, deep layer heating, medicine injection and anti-cancer drugs. According to the 'principle scheme of disease generation and development' in an explanatory memorandum, the cancer diffusion transfer therapeutic equipment aims at the fact that the reasons for generation of cancer diffusion transfer are function degradation and structural pathological changes of system levels of a human body, enables a recovery system level function therapy and a system level structural anti-damage therapy in which the chemoradiotherapy operation is used for killing the cancer cells to be combined in a symmetrical, equivalent, and mutually promoting mode to form an integrated therapeutic method aimed at the reasons, and therefore achieves the targets of controlling and eliminating the cancer diffusion transfer.

A generalized method for optimizing the global placement of a VLSI chip across multiple cost metrics, such as total wire length, timing, congestion, and signal integrity is described. The method relies upon a “look ahead” technique, combined with any generic cost function that can be used to set placement directives. These placement directives include net weights and cell spreading. The method of performing the placement involves the iterative reuse of the process of successive partitioning. This iterative reuse establishes the capability of looking ahead to determine what is to happen. Based on the look ahead, it is possible to evaluate the qualities of the placement about to be generated. The method proceeds through the placement from while maintaining the current state of the placement along with the look-ahead state of the placement. Directives are generated and modified in order that the next steps applied to the current state of the placement will cause it to change to achieve an ultimate higher quality final output.

Presuming a case of dividing a control plane of a base station controller (RNC) into an apparatus to control a cell resource and an apparatus to control each of mobile stations (MS), it is a problem to be solved to distribute a load on the mobile station controller by setting an additional mobile station controller due to an increase in subscribers. Cell control radio control servers (Cell control RCS) 7a, 7b are nodes to control common resources, e.g., radio common channel, cell spread code, electric power, etc., bound to a cell, mobile station control radio control servers (Serving RCS) 8a, 8b are nodes to control each of mobile stations (MS) 5, and movement control / location management of the mobile stations (MS) 5, radio individual channel control, etc. are performed. Cell control radio bearer server (Cell control RBS) 9a, 9b process traffic flowing on a radio common channel according to an instruction of the cell control radio control servers (Cell control RCS) 7a, 7b, and mobile station control radio bearer servers (Serving RBS) 10a, 10b process traffic flowing on the radio individual channel according to an instruction of the mobile station control radio control servers (Serving RCS) 8a, 8b.

The invention is based on the realization that brain cancer cells spread preferentially along paths of elevated water diffusion, such as along nerve fiber bundles, that can be measured by magnetic resonance (MR) diffusion-weighted imaging (DWI) and the migration of cancer cells away from the primary tumor can be predicted using computational models that incorporate DWI information. The invention therefore applies DWI and models cell migration to develop appropriate non-symmetric margins for radiation treatment of malignant brain tumors.

The invention provides a medicinal compound for improving the immunity of a human body as well as a preparation method and application of the medicinal compound. The medicinal compound comprises the following components: algae-selenium polysaccharide, cordycepin, cordyceps polysaccharide, ganoderan, lycium barbarum polysaccharide, lentinan, codonopsis pilosula polysaccharide, resveratrol, a lily extract, a date extract and xanthone. The medicinal compound has the functions of improving cell function activity, rapidly improving the immunity of the human body, protecting and repairing cells, promoting marrow to generate thrombocyte, hematokrit and leukocyte, cancer cell diffusion can be effectively inhibited, the cancer cell activity can be degraded, and malignant tumor recurrence and metastasis can be effectively inhibited. According to the preparation method of the medicinal compound, the medicinal compound is prepared into an oral liquid which is convenient for patients to use and is convenient to carry about.

The present invention provides a hybrid hydrogel stabilized by multivalent cross-linking domains. The hydrogel combines inorganic nanoparticles along with an organic polymer network. The resulting material has reinforced mechanical properties and significant mineralization, and affords sustained long-term release of ions. Furthermore, ions released from the hydrogel can enhance cell spreading and promote the osteogenic differentiation of implanted cells. These promising results indicates that the provided compositions and methods are particularly appealing for bone regenerative applications.

This method comprises the following steps: - taking a sample of a cell solution from a sample bottle, - placing said sampled solution in a settling chamber (2) placed above the analysis plate (4), - leaving the solution to settle out in order to obtain cell spreading on the analysis plate (4). It comprises a step of measuring the cell density of the sampled solution, said measurement being carried out in the settling chamber (2). The invention also relates to a device for producing a cell analysis plate which makes it possible to implement such a method.

Peptides comprising an Rpt1 domain of an INI1 / hSNF5 which inhibit HIV-1 production in a human cell, and vectors encoding those peptides are provided. Also provided are methods of inhibiting HIV-1 production in a cell, or spread of the HIV-1 to another cell, by treating the cells with the above peptides or vectors. Other methods of inhibiting HIV-1 production in a cell, or spread of the HIV-1 to another cell, by inhibiting production of INI1 / hSNF5 are provided. Additionally, methods of determining whether a test compound inhibits HIV-1 virion production in a mammalian cell, or spread of the HIV-1 to another cell, are provided. Those methods comprise determining whether the test compound inhibits the production of INI1 / hSNF5 or disrupts the interaction of HIV-1 integrase with INI1 / hSNF5.

The invention relates to a surface-modified porous-tantalum biological material and a preparation method thereof, and belongs to the technical field of biological medical materials. The surface-modified porous-tantalum biological material and the preparation method have the beneficial effects that an electrochemical anodic oxidation technology is adopted for modifying the surface of a tantalum sheet to prepare a porous tantalum-pentoxide layer with a micro-nano-scale three-dimensional through porous structure, the pore-diameter size can be controlled, the distribution and the communication ofpores are good, and a hydroxyapatite layer is further constructed on the surface of an oxidation layer, so that protein absorption and cell diffusion and proliferation are obviously enhanced, the biocompatibility and the osteoconduction are improved, the microenvironment beneficial to cell growth is generated, the adhesion, the proliferation, the differentiation and the mineralization of primary osteoblasts of human are promoted, the growth of bone tissues into the porous tantalum is promoted to form a unique bone-implant interface, the stability and the function of the implant in the bone tissues are obviously enhanced, the osseointegration of the bone-implant interface is accelerated, and the stability of the implant into the bone tissues is obviously enhanced. The surface-modified porous-tantalum biological material has the advantages of high corrosion resistance and bright application prospect.

An object of the present invention is to provide a method of detecting rare cells in which, upon spreading a cell suspension in a flow path formed on a microchamber chip, the loss of rare cells is reduced by improving the cell recovery rate. The present invention provides a method of detecting rare cells from a cell suspension by using a cell-spreading device 10 comprising a microchamber chip 1, a flow path-forming frame 2, an inlet port 3, and an outlet port 4; and a method of recovering rare cells comprising: the step (X) of introducing a cell suspension to a flow path 5 via the inlet port 3 so as to spread cells in the flow path 5 on the microchamber chip 1; and the step (Y) of storing the cells spread in the flow path 5 on the microchamber chip 1 in microchamber 6s by intermittent liquid feeding.

The invention rates to a hepatitis C virus (HCV) cDNA-based culture system capable of synthesis of infectious HCV in cell culture and cell-to-cell spread of the virus. The invention also relates to a method of measuring the level of HCV infection in a hepatocyte cell. A method for identifying a modulator of HCV activity is also presented, and a method for modulating HCV activity. The invention provides a reliable system for both genetic analysis of the viral genome and for the development of novel antiviral strategies.

A cell-spreading device may include a microchamber chip having a microchamber capable of enclosing and retaining a cell, a channel-forming frame united with the microchamber chip to form a channel on the microchamber, an inlet provided in the channel-forming frame to allow a cell suspension to flow into the channel, and an outlet provided in the channel-forming frame to allow the cell suspension, which has been allowed to flow into the channel through the inlet, to flow out from the channel. When an aperture of the microchamber is projected perpendicularly to a longitudinal width of the microchamber chip, the void ratio that is a ratio of the sum total of voids to the longitudinal width is not more than 5%, the void being a length of a portion where the projected aperture of the microchamber is not present against the longitudinal width.

The invention discloses a breast cancer metastasis therapeutic device which can integrate pointed restoration function treatment and surgery chemoradiotherapy and prevent and treat breast cancer metastasis. The breast cancer metastasis therapeutic device is composed of vibration squeezing friction heads, an adjustable switch, a semi-circular fixer and a square fixer, belongs to the advanced therapeutic technology integrally combining laser, ultrasonic waves, microwaves, therapeutic massage and anti-cancer drug injection and performs treatment for diseased region function restoration and cancer cell spreading prevention. According to a breast cancer metastasis emergence and development principle schematic diagram and a breast cancer metastasis therapeutic principle and technique schematic diagram in a manual, human body system level function decreasing and system level structural damage symmetric mutual restriction causes breast cancer metastasis. By adopting the breast cancer metastasis therapeutic device, system level function decreasing part treatment and surgery chemoradiotherapy cancer cell killing system level structural damage resistant treatment are mutually symmetrically and equivalently promoted and are integrally combined to form an etiological treatment mode, and therefore the treatment purpose of controlling and eliminating breast cancer metastasis is achieved.

Composite constructs of polymer matrices and polymeric microgels, and methods of making and using thereof, are described. The addition of the microgels to polymeric matrices retains the overall mechanical properties of the matrices and enables cell spreading, invasion and vascularization. Using fibrin as a model system, composite fibrin constructs with microgels are described, in which microgels form unique interconnected pockets within the fibrin matrix. The microgel assembly is driven by the polymerization dynamics of fibrin, and the architecture of these interconnected pockets can be tuned. The composite constructs support cell spreading, migration and infiltration more efficiently than do control matrices. The composite constructs can be used attract cells and / or to deliver therapeutic, diagnostic, nutraceutical, prophylactic and cosmetic agents to a desired site.

Methods and techniques for controlled printing of a cell sample for karyotyping are provided. The methods can involve matrix printing using on-the-fly printing or dispensing to accurately spread cells within at least one cell sample on a surface in preparation for karyotyping, and further analysis. Advantageously, the methods result in a uniform distribution of chromosomes of the cell suspension or sample on the surface of a substrate which can be substantially discretely identified, and also provide for efficiency in a subsequent staining process and any further analysis of the stained chromosomes using a microscope or other imaging device.

The embodiments herein disclose a method of fabricating composite scaffolds for skin tissue regeneration. The methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA) are synthesized. The poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are synthesized. The hydrogel is synthesized. The composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds is fabricated. A plurality of physico-chemical characteristics of the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are analysed. The physico-chemical characteristics comprises mechanical properties, swelling ratio and enzymatic degradation and scanning electron microscope imaging. The fibroblast cells are encapsulated within the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds and hydrogels. The fibroblast cells are seeded on composite scaffold and PGS-PCL scaffold. The fibroblast cell viability, fibroblast cell attachment, fibroblast cell spreading, fibroblast cell proliferation and fibroblast cell metabolism are analysed in composite scaffolds, PGS-PCL scaffolds and hydrogels.

The invention discloses a polypeptide for inhibiting the in-vivo diffusion transfer effect of tumor cells and a preparation method and application thereof. The polypeptide consists of 25 amino acids, and its amino acid sequence is shown as SEQ ID No.1. The polypeptide is synthesized by utilizing a solid phase chemical synthesis method, the yield is high, the process is stable, and the molecular weight is 2,777.26 Da. The polypeptide has the activity of inhibiting the proliferation and metastasis of tumor cells and is safe and effective in tumor treatment. Therefore, the polypeptide has the important application value in clinical antitumor therapy.

A method of low temperature induction off-spreading of cell for dynamic measurement of medicament influence on cell spreading The method is characterized by comprising the following steps: planting adherent cells in a suspension state on a culture aperture or plate and incubating in an incubator to complete spread the cells; adding medicament and incubating the cells and the medicament together for 20 min-1 h and removing the medicament; placing the cells treated by the medicament under a microscope and shooting a morphology image before cell spreading; absorbing the aqueous solution and adding 4 DEG C cell nutrient solution or buffer; dynamically observing off-spreading state of the cells by a microscope immediately, continuously obtaining morphology images of the cells every few minutes for 30 min-1 h; measuring adherence area of each cell or a mean value of adherence areas of all the cells at a certain time point by a piece of software; and comparing the obtained data with blank data and analyzing to obtain data of medicament influence on cell spreading. The invention has advantages of simple process, no damage on cells, rapid detection, dynamic observation or measurement and good effect.

The present invention provides fully human antibodies that bind to respiratory syncytial virus F protein, compositions comprising the antibodies and methods of use. The antibodies of the invention are useful for preventing fusion of the virus with the cell membrane and preventing cell to cell spread of the virus, thereby providing a means of preventing the infection, or treating a patient suffering from the infection and ameliorating one or more symptoms or complications associated with the viral infection. The antibodies may also be useful for diagnosis of an infection by RSV.

The invention relates to a method for quantitatively measuring cell spreading rate based on idealized cell radius. The method is characterized by comprising the steps of: planting adherent cells in a suspended state on culture holes or plates, capturing the morphology of the cells at different time points by using imaging equipment during the cell adhering and spreading process, measuring the adhering area of each cell, idealizing the cells before and after spreading to be round, and calculating the cell spreading rate (growth rate of the cell radius within unit time). According to the invention, the idealized cell radius is used as a standard, the spreading rate is calculated according to the cell spreading time and the change of the cell radius, and the defects of the traditional method are solved; and compared with the traditional cell spreading measuring method, the method has the advantages that the method is more accurate, the required measuring equipment is simple, and the calculation method is simple and feasible, and can be integrated to measuring equipment.

The present invention provides Listeria that are attenuated for entry into non-phagocytic cells as well as a variety of methods of inducing immune responses involving administering compositions comprising the attenuated Listeria. Some of the attenuated Listeria are mutant Listeria that comprise at least one mutation in a gene encoding an invasin, such as an internalin. Some of the attenuated Listeria are further attenuated for cell-to-cell spread. Pharmaceutical compositions and vaccines useful in the methods of the invention are further provided. Methods of making and improving vaccines are also provided.

The invention discloses a cell tracking method based on local graph matching and a convolutional neural network. The method comprises the following steps: S1, segmenting a cell image by adopting a watershed method; S2, constructing and training a convolutional neural network, and extracting the depth similarity of the to-be-matched cell pair by using the trained convolutional neural network; S3, extracting the local triangular map similarity of the to-be-matched cell pair from the cell segmentation image; S4, establishing a similarity matrix by combining and extracting the depth similarity andthe local triangular map similarity of the to-be-matched cell pair, and taking the cell pair corresponding to the maximum value of the similarity matrix as a seed cell; and S5, starting from the obtained seed cells, sequentially matching the adjacent cell pairs by adopting a neighborhood cell diffusion method until all cells are matched. According to the method, the convolutional neural network is introduced to extract the depth similarity of the to-be-matched cell pair, and the cells are tracked by combining the depth similarity and the local triangular map similarity, so that the method hasthe characteristics of wide application range and high tracking accuracy.

Provided herein are methods and related systems for controlling droplet spreading on a surface, including droplets in which a biological component is suspended. A biological solution is provided as a droplet to a surface. Interference fringes generated by the droplet on the surface are imaged, wherein the imaging is over a time course during which the droplet spreads on the surface. A droplet parameter is determined from the imaging step and a process parameter controlled to obtain an interference fringe pattern corresponding to a desired droplet parameter. In this manner, well-controlled droplet spreading is achieved, which is important in a range of applications, including assays that rely on good metaphase spreading.

An object of the present invention is to provide a means that enables improvement of efficiency in recovery of rare cells in a cell suspension by suppressing cell adsorption to laboratory equipment but not suppressing cell adsorption to a cell spreading substrate. A cell spreading method according to the invention includes a step of adding to a cell suspension a protein in such an amount as to give a final concentration of from 0.0001% to 0.1%, and preferably from 0.0005% to 0.01% (a protein addition step) and a step of spreading cells contained in the cell suspension that has been subjected to the protein addition step on a cell spreading substrate having a contact angle with water of 60 degrees or more, and preferably from 70 to 90 degrees, and allowing to stand to cause the cells to adsorb onto the substrate (a cell spreading step).

The invention discloses an intelligent community management system based on the TCP / IP protocol, which is characterized in that: the hardware structure of the digital home server (DHS) mainly includes an embedded network chip IP2022, and a power circuit connected thereto, a data storage circuit, expansion port circuit, Ethernet communication module, alarm circuit module, remote control module, pulse input module; there is a home page in the DHS, which can be logged in with the help of the Internet and a browser to monitor household appliances ; DHS is connected to the community management center through the community LAN, and can transmit the historical data recorded in the family to the community management center through the community LAN, and can also receive information from the management center server, and feed it back to the user through the home page. The present invention adopts embedded network chip IP2022 to realize network connection function, built-in HTTP, SMTP, TCP / IP and other network protocols; the researched and developed digital home server (DHS) and community management information system can form an Internet-based residential community intelligent centralized The management and remote monitoring system has the functions of house anti-theft, disaster and help alarm, multi-meter remote automatic transcription of water, electricity and heating.

The invention discloses an inonotus obliquus magnetized enzyme. The inonotus obliquus magnetized enzyme includes the following constituents in parts by weight: biological enzymes 1.5-3, magnetized water 51-59, inonotus obliquus 15-30, longans 10-30, fructus lycii 5-20, jujubes 8-15, ganoderma lucidum 8-15 and honey 3-7. The magnetized enzyme provided by the invention is prepared from the constituents like the inonotus obliquus, the fructus lycii and the longans, contains a large amount of functional components like plant fiber polysaccharides, alkaloids and steroid having the functions of resisting cancer, decreasing blood pressure, decreasing blood glucose, decreasing blood lipids and reactivating immunity and can improve the vitality of immune cells and inhibit the spread and reoccurrence of cancer cells; and moreover, the magnetized enzyme can prevent the stomach and intestines from absorbing harmful substances and has the efficacy of maintaining beauty and keeping young.