Method for quantitatively measuring cell spreading rate based on idealized cell radius

A quantitative measurement and cell technology, applied in measurement devices, material analysis by optical means, instruments, etc., can solve the problems of difficult, rough, and no quantitative measurement of spreading rate of cells, and achieve the effect of simple calculation method and simple measurement equipment.
CN102507580AInactive Publication Date: 2012-06-20NANCHANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANCHANG UNIV
Publication Date
2012-06-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for quantitatively measuring cell spreading rate based on idealized cell radius. The method is characterized by comprising the steps of: planting adherent cells in a suspended state on culture holes or plates, capturing the morphology of the cells at different time points by using imaging equipment during the cell adhering and spreading process, measuring the adhering area of each cell, idealizing the cells before and after spreading to be round, and calculating the cell spreading rate (growth rate of the cell radius within unit time). According to the invention, the idealized cell radius is used as a standard, the spreading rate is calculated according to the cell spreading time and the change of the cell radius, and the defects of the traditional method are solved; and compared with the traditional cell spreading measuring method, the method has the advantages that the method is more accurate, the required measuring equipment is simple, and the calculation method is simple and feasible, and can be integrated to measuring equipment.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and is a method for measuring the spread rate of adherent cells and the influence of various drugs on the cell spread rate. technical background

[0002] Cell spreading is one of the important physiological activities or functions of adherent cells, and many human physiological activities or diseases are related to cell spreading. For example, vascular endothelial cells build the inner wall of blood vessels or repair the damaged vascular endothelial layer through cell spreading. Therefore, changes in endothelial cell spreading are often related to the occurrence, development and tumor metastasis of cardiovascular diseases, and the effect of drugs on cell spreading It must also be one of the important indicators in in vitro cell tests to evaluate the efficacy of drugs.

[0003] Unfortunately, the mechanism of cell spreading, especially the effect of drugs on cell spreading, is not well understood, an...

Claims

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