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Tissue culture method for gynura bicolor leaf and culture medium special for same

A technology of S. japonica and culture medium, which is applied to the field of plant tissue culture, can solve the problems such as the tissue culture of S. japonicus, and achieves the effects of small variability, high genetic stability and expansion of reproduction coefficient.

Inactive Publication Date: 2010-09-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the tissue culture of G. purple-backed leaves as explants at home and abroad.

Method used

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  • Tissue culture method for gynura bicolor leaf and culture medium special for same
  • Tissue culture method for gynura bicolor leaf and culture medium special for same
  • Tissue culture method for gynura bicolor leaf and culture medium special for same

Examples

Experimental program
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Embodiment 1

[0034] Embodiment 1, the acquisition of regenerated seedlings of G. purple-backed and its statistical observation

[0035] 1. Preparation of callus tissue

[0036] 1) Pretreatment of explants

[0037] Select tender and flat leaves of Gelonia purpurea (the variety is red leaves, purchased from the Vegetable Center of Beijing Academy of Agriculture and Forestry Sciences) as explants, first sterilize in 70% (volume percentage) alcohol for 30 seconds, and rinse with sterile water for 5 seconds. Once again, soak for 8 minutes with 0.1% (volume percent) mercuric chloride added dropwise with Tween, and then rinse with sterile water.

[0038] 2) Acquisition of callus

[0039] Cut the leaves of G. chinensis pretreated in the above step 1) into about 1cm×1cm (the cutting method is as follows: figure 1 Shown) small piece, the leaf back is inoculated on the medium for inducing callus of the leaves of G. purpurea leaf, at the temperature of 26 ℃, the light intensity is 2500lux, and the ...

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Abstract

The invention discloses a tissue culture method for gynura bicolor leaf and a culture medium special for the same. The culture medium for gynura bicolor leaf callus induction is a solid culture medium obtained by adding 2,4-D, 6-BA, a carbon source and gel into MS basic culture solution, wherein the final concentration of the 2,4-D in the culture medium is between 1 and 3mg / L; and the final concentration of the 6-BA in the culture medium is between 0.2 and 1mg / L. The method of the invention comprises the following steps of: putting a gynura bicolor leaf on the culture medium for callus induction for culturing to obtain callus; carrying out differentiation culture to obtain clustered shoots; and carrying out root induction on the clustered shoots to obtain regenerated shoots. Due to the method of the invention, a classic gynura bicolor tissue culture system is established, breeding is carried out by using the gynura bicolor leaf as an explant, materials are readily available and sources are sufficient and masses of gynura bicolor regeneration plants can be induced at high frequency; the prepared plants are easy to root; the regeneration plants have high survival rate; and the maximum survival rate can reach over 95 percent.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for tissue culture of leaves of G. purple-backed and a special culture medium thereof. Background technique [0002] Gynura bicolor, scientific name Gynura bicolor DC, also known as purple back vegetable, two-color Sanqi grass, red back vegetable, Guanyin vegetable, is a perennial herbaceous plant of the genus Panax notoginseng in the Compositae family. The whole plant is purple-green, with long egg-shaped leaves and serrated edges. The leaf surface is green and slightly purple, and the back is purple-red waxy and shiny. It is a wild vegetable with medicinal value. As a high-nutrition and health-care vegetable worthy of promotion, Gastronia violet contains relatively rich and comprehensive nutrients. In addition to the nutrients of ordinary vegetables, it is also rich in nutrients that can improve animal disease resistance, and has a moderate effect on malignant growt...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郭仰东刘莉莎温常龙赵永钦赵立群王玉珏程琳
Owner CHINA AGRI UNIV
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