Application of adenovirus mediated interleukin-24 for preparing radiotherapy hypersitization medicine

A technology of interleukin and adenovirus, applied in drug combination, gene therapy, antineoplastic drugs, etc., can solve the problem that Ad-IL-24 radiosensitizer has not been reported.

Inactive Publication Date: 2010-12-15
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the application of Ad-IL-24 as a radiosensitizer has not been reported at home and abroad.

Method used

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  • Application of adenovirus mediated interleukin-24 for preparing radiotherapy hypersitization medicine
  • Application of adenovirus mediated interleukin-24 for preparing radiotherapy hypersitization medicine
  • Application of adenovirus mediated interleukin-24 for preparing radiotherapy hypersitization medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Cell culture: SPC-A1 cells use RPMI1640 complete medium (10% FCS), at 37 ° C, 5% CO 2 under cultivation. Subculture once every 2 to 3 days.

[0032] (2) Determination of amplification and titer of AdV, Ad-IL-24 adenovirus

[0033] Infect 70% of the adherent QBI-293A cells with AdV and Ad-IL-24, collect the cells after 48 hours, freeze and thaw the cell suspension at -80°C and 37°C four times, take the supernatant, and repeatedly amplify, the virus Store at -80°C.

[0034] Culture and grow QBI-293A cells in good condition, digest with trypsin, count the cells, and dilute the cell concentration to 10 5 cells / ml, inoculate the cells at 100 μl per well on a 96-well plate, and after culturing for 24 h, the harvested recombinant virion was injected at 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 After dilution, inoculate 1 row at 100 μl per well for each dilution, at 37°C, 5% CO 2 After culturing in the cell incubator for 18 hours, the fluorescence was counted under a fluor...

Embodiment 2

[0065] Example 2, the tumor inhibitory effect of Ad-IL-24 and combined radiotherapy on the transplanted tumor of SPC-A1 lung adenocarcinoma in nude mice.

[0066] (1) Establishment of SPC-A1 human lung adenocarcinoma tumor-bearing nude mouse model

[0067] SPC-A1 human lung adenocarcinoma cells were completely cultured with RPMI1640 complete medium (10% FCS) in 5% CO2, and conventionally cultured at 37°C. The SPC-A1 human lung cancer cells in the logarithmic growth phase were washed with PBS, digested with 0.25% trypsin, stopped with serum-free medium, centrifuged at 1000r / min for 5min to harvest the cells, and adjusted the cell concentration after counting the cells. 3×10 7 / ml of cell suspension. Twenty-five 4-week-old SPF grade BALB / c male nude mice were routinely disinfected with Aneriodine in the right anterior armpit of the nude mice, and each nude mouse was subcutaneously injected with a cell suspension of 3.0×10 6 / 100μl, and observe the growth and tumor formation o...

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Abstract

The invention belongs to the filed of radiotherapy hypersitization medicine, in particular to application of adenovirus mediated interleukin-24 gene in the filed of radiotherapy hypersitization medicine. The invention also discloses a method of applying adenovirus mediated interleukin-24 gene expression vectors Ad-IL-24 for enhancing the sensitivity of tumor cells on radiotherapy, which comprises the following steps: before radiotherapy, introducing the mediated interleukin-24 gene expression vector Ad-IL-24 to the tumor cells to express human IL-24 gene in the tumor cells, and then radiotherapy is performed. In the invention, the adenovirus mediated IL-24 single gene expression has the functions of significantly inhabiting the growth of SPC-A1 lung adenocarcinoma cells and inducing cell apoptosis; Ad-IL-24 adjuvant radiotherapy has higher cytotoxicity on SPC-A1 lung adenocarcinoma cells and higher effect on inducing apoptosis compared with Ad-IL-24 simple group and single radiotherapy group, and both of the functions show obvious radiotherapy hypersitization synergy effect.

Description

technical field [0001] The invention belongs to the field of radiotherapy sensitization drugs, in particular to the application of adenovirus-mediated interleukin-24 gene in the field of radiotherapy sensitization. Background technique [0002] Radiation therapy is one of the routine means of tumor treatment, and 70% to 80% of malignant tumor patients need to receive radiotherapy during the course of treatment. Tumor insensitivity or resistance to radiation is one of the main reasons why radiation therapy is difficult to cure malignant tumors. In recent years, with the in-depth research on the molecular mechanism of tumor pathogenesis and the mechanism of cell growth regulation, some exciting results have been achieved in tumor gene therapy, but the transgene therapy targeting a single link often cannot achieve satisfactory therapeutic effects. Weichselbaum et al. proposed a new idea of ​​tumor gene-radiation therapy in 1994. The principle is to couple the regulatory sequen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00
Inventor 杨吉成盛伟华凌春华黄锦宏谢宇锋赵大国
Owner SUZHOU UNIV
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