Agent ,which is convenient for sampling, for measuring glycosylated hemoglobin percentage

A technology for glycosylated hemoglobin and detection reagent, applied in the field of medical immunity, can solve the problems of short stable period of antibody working solution, unfavorable daily use, and difficulty in obtaining low-temperature refrigerators, and achieves the effect of simplifying operation steps and prolonging effective use time.
CN101915849BActive Publication Date: 2013-06-05SHENZHEN GOLDSITE DIAGNOSTICS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENZHEN GOLDSITE DIAGNOSTICS
Publication Date
2013-06-05

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Abstract

The invention relates to a method for measuring glycosylated hemoglobin content, which comprises the following steps of: a, uniformly mixing a whole blood sample and hemolytic liquid; b, adding an appropriate amount of hemolyzed sample into latex suspending in glycine buffer solution; c, respectively adding an antibody B reagent and an antibody A reagent after preset time; d, uniformly mixing andreading a scattering value through a specific protein analyzer under the condition of single-wavelength light irradiation; and e, reading the glycosylated hemoglobin content from a standard curve diagram according to the scattering value. The glycosylated hemoglobin content of the blood sample can be directly determined without separately measuring the total hemoglobin content and the glycosylated hemoglobin content of the blood sample by taking fixed-time nephelometry as a detection principle; and antibody working solution does not need preparing in advance, so that the effective service time of the reagents is prolonged, the operating steps are simplified and the measuring time of the sample is shortened.
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Description

technical field

[0001] The invention relates to medical immunization, in particular to a method capable of directly measuring the percentage of glycosylated hemoglobin and its reagent. Background technique

[0002] Glycosylated hemoglobin (HbAlc) is the product of the combination of hemoglobin and blood sugar in red blood cells in human blood. Glycosylated hemoglobin can stably and reliably reflect the average blood sugar level within 120 days before the test, and is not greatly affected by factors such as blood draw time, fasting, and insulin use. Therefore, the International Diabetes Federation has launched a new edition of the Asia-Pacific Diabetes Prevention Guidelines, which clearly stipulates that glycosylated hemoglobin is the internationally recognized "gold standard" for diabetes monitoring. If the fasting blood sugar or postprandial blood sugar is not well controlled, it is impossible for the glycosylated hemoglobin to reach the target.

[0003] Commonly used met...

Claims

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