36results about How to "Easy to add sample" patented technology

The invention discloses an alternative high-throughput electroporation device. The electroporation device comprises a substrate, wherein multiple through holes are formed in the substrate; a pair of electrodes is arranged at each through hole; the electrodes are connected with a wire arranged on the substrate; the electrodes can be gated in a single-point mode. The provided electroporation device can be manufactured by using a printed circuit board (PCB) manufacturing method. The electroporation device can be used for performing the electroporation on cells, such as animal cells or bacteria. During use of the device, an electroporation pulse is applied to an electroporation system in the through holes through the electrodes at the through holes. The electroporation device can be manufactured by using a PCB process, is mature in technology and low in cost and can be produced in batches. The electroporation device manufactured by using the PCB process can be seamlessly connected with a PCB-based circuit control system, and the electroporation device contributes to realizing high-throughput automatic gene function screening based on the electroporation.

The invention discloses a double centrifugal tube. Two centrifugal tubes are coaxially arranged, the bottom end of one centrifugal tube is in threaded connection with the top end of the other centrifugal tube, the bottoms of the tube bodies are in a conical shape, and the bottom ends of the tube bodies are provided with spherical containing bodies communicated with the tube bodies. The double centrifugal tube is simple in structure, easy and convenient to use and high in centrifugal efficiency.

The invention discloses a cabin door structure. The cabin door structure comprises a sample adding hole formed in a top cover and a cabin door covering the outer side of the sample adding hole, wherein a bulge is arranged on the cabin door; the bulge extends inwards to extend into the sample adding hole when the cabin door covers the sample adding hole; and the distance between the inner side faceof the bulge and the inner side face of the top cover is smaller than the thickness of a sample adding target plate. According to the cabin door structure disclosed by the invention, the distance between the inner side face of the bulge and the inner side face of the top cover is set to be smaller than the thickness of the sample adding target plate, so that when the sample adding target plate isnot correctly mounted, the inner side face of the bulge and the sample adding target plate generate interference, and the cabin door cannot be normally closed; the cabin door structure disclosed by the invention can be used for detecting whether the sample adding target plate is correctly mounted in a target plate groove or not; and furthermore, when a mounting position of the sample adding target plate has a slight error, the bulge can be pressed on the sample adding target plate in a cabin door closing process and the sample adding target plate is pressed to be correct, so that the sample adding target plate is correctly mounted in the target plate groove and the cabin door structure also has the effect of correcting the error.

The invention relates to a method for measuring glycosylated hemoglobin content, which comprises the following steps of: a, uniformly mixing a whole blood sample and hemolytic liquid; b, adding an appropriate amount of hemolyzed sample into latex suspending in glycine buffer solution; c, respectively adding an antibody B reagent and an antibody A reagent after preset time; d, uniformly mixing andreading a scattering value through a specific protein analyzer under the condition of single-wavelength light irradiation; and e, reading the glycosylated hemoglobin content from a standard curve diagram according to the scattering value. The glycosylated hemoglobin content of the blood sample can be directly determined without separately measuring the total hemoglobin content and the glycosylated hemoglobin content of the blood sample by taking fixed-time nephelometry as a detection principle; and antibody working solution does not need preparing in advance, so that the effective service time of the reagents is prolonged, the operating steps are simplified and the measuring time of the sample is shortened.

The invention relates to a simulated on-site cultivation system for measuring marine primary productivity by using a black-and-white bottle sampling method, comprising a cultivation unit, a temperature and water flow simulation control unit, and a lighting simulation control unit; the cultivation unit includes several cultivation subunits, and the cultivation subunits The unit includes a cylinder-shaped water bath cover, a central rotating shaft is arranged along the central axis of the cylinder, and a culture bottle rotating bracket is arranged on the periphery of the central rotating shaft. Several culture bottles are detachably fixed on the culture bottle rotating bracket, and each culture subunit Set in series with the central rotating shaft as the axis, there are gaps between the culture sub-units; the temperature and water flow simulation control unit includes a circulating water bath, which is connected to the water inlet and water outlet on the water bath cover respectively; the lighting simulation control unit It includes a light panel and a fluorescent tube, the fluorescent tube is arranged on the light panel, the fluorescent tube is connected to a power supply, and the lighting simulation control unit is set outside the outermost cultivation sub-unit.

The invention discloses a group of real-time fluorescent QRT-PCR primers capable of detecting cattle, sheep akabane virus by a one-step method, a probe and a usage method thereof. The nucleotide sequences of the described primers and the probe are respectively as shown in SEQIDNO.1-3. The method for detecting the cattle, sheep akabane virus is as follows: sample RNA is used as template, the above primers and probe are adopted to perform the one-step real-time fluorescent QRT-PCR amplification test of the sample RNA, after reaction, if a specific fluorescent amplification curve appears, the sample RNA is judged to contain the cattle, sheep akabane virus nucleotide, namely the sample is tested positive for the cattle, sheep akabane virus. A standard curved prepared according to analog standards can quantify the one-step real-time fluorescent QRT-PCR amplification test. The method for detecting the cattle, sheep akabane virus is safe, quick, high in specification and sensitivity, and provides technical support for detection of the cattle, sheep akabane disease in livestock husbandry and the inspection and quarantine work of the exit cattle and sheep.

The invention discloses a closed PCR amplification tube for integrated gene detection, which includes a tube body for containing PCR buffer, a liquid storage pool for containing hybridization buffer, and a cap for sealing the upper opening of the liquid storage pool. The liquid storage tank is put into the upper part of the pipe body, and the upper end of the liquid storage tank is airtightly connected with the opening upper end of the pipe body. The invention not only enables PCR, gene hybridization, and gene detection to be completed in a closed system, avoiding cross-contamination, but also completely isolates the PCR amplification system and DNA hybridization system, and avoids components such as buffers and enzymes in the amplification system The impact on the hybridization reaction greatly improves the accuracy and repeatability of genetic testing. The tube cap in the closed PCR amplification tube of the present invention makes adding samples easier, has stronger sealing performance, and has better detection accuracy.

The invention discloses a closed PCR amplification tube for integrated detection of genes, comprising a tube body for containing PCR buffer, a liquid storage pool for containing hybridization buffer, and a tube with an upper opening of the sealed liquid storage pool Cover, the liquid storage tank is put into the upper part of the tube body, the upper end of the liquid storage tank is airtightly connected with the opening upper end of the tube body, there is a gap between the outer side of the liquid storage tank and the inner wall of the tube body, and the upper part of the liquid storage tank is provided with The through hole that communicates the void with the inside of the liquid storage tank. The invention not only enables PCR, gene hybridization, and gene detection to be completed in a closed system, avoiding cross-contamination, but also completely isolates the PCR amplification system and DNA hybridization system, and avoids components such as buffers and enzymes in the amplification system The impact on the hybridization reaction greatly improves the accuracy and repeatability of genetic testing. The tube cap of the invention makes adding samples easier, has stronger sealing performance, and has better detection accuracy.

The invention discloses a high pressure extrusion and filtration device for liposome. The device comprises a bottomless extrusion filter body, the bottomless extrusion filter body is a cavity with the upper portion in a circular-tube shape and the lower portion in a conical shape, a sample feeding opening is formed in the upper end of the bottomless extrusion filter body, the sample feeding opening is movably connected with a screw cap, the device further comprises a filter bottom, and the filter bottom is a cylinder provided with the bottom, wherein the upper end of the filter bottom is open, a discharging pipe is arranged at the bottom of the filter bottom, centripetal protruding blocks are arranged on the inner surface of the side wall of the filter bottom, and a lower supporting network, a filter membrane and an upper supporting network are movably connected to the protruding blocks in sequence. By means of the device, liposome samples can be extruded multiple times, operation time is shortened, and convenience and high efficiency of large-scale liposome preparation in the laboratory are achieved. High efficiency is particularly embodied when the same sample is in need of multiple times of filtration operation. The device is not prone to air leakage under high pressure gas, and therefore reliability of an extrusion filter is improved. The high pressure extrusion and filtration device is simple in structure, and high temperature sterilization can be conducted on the extrusion filter easily.