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36results about How to "Easy to add sample" patented technology

Gene-detection integrally completed enclosed PCR (polymerase chain reaction) amplification tube

The invention discloses a gene-detection integrally completed enclosed PCR (polymerase chain reaction) amplification tube which comprises a tube body, a solution storage tank and a tube cover, wherein the tube body is used for accommodating a PCR buffer solution, the solution storage tank is used for accommodating a hybridized buffer solution, the tube cover encloses an opening on the upper side of the solution storage tank, the solution storage tank is placed at the upper part of the tube body, the upper end of the solution storage tank is hermetically connected with the upper end of the opening of the tube body, the bottom surface of the solution storage tank is provided with a communicating tube for communicating the upper and lower sides of the bottom surface, and the lower end of the communicating tube is connected with the bottom surface. According to the invention, PCR, gene hybridization and gene detection can be completed in an enclosed system, thereby avoiding cross contamination; and PCR amplification system is completely isolated from a DNA hybridization system, thereby avoiding the influence of compositions such as buffer solutions and enzyme and the like in the amplification system on hybridization reaction, and greatly improving the accuracy and repeatability of gene detection. Due to the tube cover of the enclosed PCR amplification tube disclosed by the invention, the sample adding is more convenient, the tightness is strong, and the detection accuracy is better.
Owner:SOUTHEAST UNIV

Automatic detection system of genotyping chip

The invention relates to the field of in-vitro diagnostic reagent detection, in particular to an automatic detection system of a genotyping chip. The detection system comprises a hybridization temperature control assembly, a pipetting assembly, a moving guide rail and a hybridization cleaning assembly, the hybridization temperature control assembly comprises a genotyping chip container, and a genotyping chip film strip can be horizontally placed in the genotyping chip container; the pipetting assembly comprises a needle discarding window, a needle turntable, a PCR product middle turntable andan image acquisition device which are sequentially arranged in the same straight line direction; the hybridization cleaning assembly comprises a liquid adding mechanism and a hybridization cleaning mechanism. The detection system has the beneficial effect that rapid and convenient suction and sample adding can be realized. According to the automatic detection system of the genotyping chip, automatic hybridization and detection are achieved, continuity of detection steps is guaranteed, the workload of manual intervention is small, and the detection efficiency is improved.
Owner:FUJIAN UNIV OF TECH

Combined type reaction cup

The invention discloses a combined type reaction cup. The combined type reaction cup comprises a preinstalled reagent bottle component and a reaction cup body, wherein the preinstalled reagent bottle component comprises a component shell and at least one reagent encapsulation bin, the bottom of the component shell extends outwards and is provided with a sample adding port, an upper elastic sealing part is arranged above the reagent encapsulation bin, and a sample adding port elastic sealing plug is arranged above the sample adding port; a bearing plate is fixed to the inner side of the upper end of the reaction cup body, a reagent hole and a sample adding hole are formed in the bearing plate, a groove is formed in the position corresponding to the reagent hole, and a lower elastic sealing part is arranged in the groove; when the preinstalled reagent bottle component is completely buckled with the reaction cup body, the bottom of the reagent encapsulation bin penetrates through the lower elastic sealing part and stretches into the reaction cup body through the reagent hole. The combined type reaction cup is high in overall sealing performance and easy to combine. Samples can be added at the same time or in sequence. Sample adding accuracy is guaranteed through positive air pressure. Meanwhile, the combined type reaction cup is compact in overall structure and convenient to popularize.
Owner:TZONE (HUNAN) BIOTECHNOLOGY LLC

Novel gel electrophoresis unit

The invention relates to a novel gel electrophoresis unit which mainly comprises an electrophoresis tank body, a mating electrophoresis tank cover, a gel preparation comb and a gel preparation platform, wherein clamping grooves are formed on the left side and the right side of the gel preparation platform; two dismountable and smooth rectangular rubber plates are fitted with the clamping grooves;a water tank is arranged at the lower end of the gel preparation platform; a water inlet and outlet valve is arranged at the bottom of the water tank; black paint is applied below the gel preparationplatform; the electrophoresis tank body is connected with an electrophoresis buffer collection bottle through a Y guide pipe; the electrophoresis buffer collection bottle is connected with a gas pumpthrough a guide pipe; and a positive electrode wire and a negative electrode wire are arranged at the left end and the right end of the electrophoresis tank cover. A gel pouring tank, a gel loading tank and an electrophoresis tank are designed integrally; contact between a user and carcinogenic dye ethidium bromide (EB) gel is reduced; degradation of nucleic acid in a gel electrophoresis process is effectively avoided by the cooling water tank; a gel electrophoresis step is simplified; gel electrophoresis operation of the user is facilitated; and the electrophoresis efficiency is improved.
Owner:GUANGZHOU MUNICIPAL ZHONGWEI BIOTECH CO LTD +2

Vacuum chamber and cabin door structure

The invention discloses a cabin door structure. The cabin door structure comprises a sample adding hole formed in a top cover and a cabin door covering the outer side of the sample adding hole, wherein a bulge is arranged on the cabin door; the bulge extends inwards to extend into the sample adding hole when the cabin door covers the sample adding hole; and the distance between the inner side faceof the bulge and the inner side face of the top cover is smaller than the thickness of a sample adding target plate. According to the cabin door structure disclosed by the invention, the distance between the inner side face of the bulge and the inner side face of the top cover is set to be smaller than the thickness of the sample adding target plate, so that when the sample adding target plate isnot correctly mounted, the inner side face of the bulge and the sample adding target plate generate interference, and the cabin door cannot be normally closed; the cabin door structure disclosed by the invention can be used for detecting whether the sample adding target plate is correctly mounted in a target plate groove or not; and furthermore, when a mounting position of the sample adding target plate has a slight error, the bulge can be pressed on the sample adding target plate in a cabin door closing process and the sample adding target plate is pressed to be correct, so that the sample adding target plate is correctly mounted in the target plate groove and the cabin door structure also has the effect of correcting the error.
Owner:ZYBIO INC

Simulated Field Culture System for Determination of Marine Primary Productivity Using Black and White Bottle Method

The invention relates to a simulated on-site cultivation system for measuring marine primary productivity by using a black-and-white bottle sampling method, comprising a cultivation unit, a temperature and water flow simulation control unit, and a lighting simulation control unit; the cultivation unit includes several cultivation subunits, and the cultivation subunits The unit includes a cylinder-shaped water bath cover, a central rotating shaft is arranged along the central axis of the cylinder, and a culture bottle rotating bracket is arranged on the periphery of the central rotating shaft. Several culture bottles are detachably fixed on the culture bottle rotating bracket, and each culture subunit Set in series with the central rotating shaft as the axis, there are gaps between the culture sub-units; the temperature and water flow simulation control unit includes a circulating water bath, which is connected to the water inlet and water outlet on the water bath cover respectively; the lighting simulation control unit It includes a light panel and a fluorescent tube, the fluorescent tube is arranged on the light panel, the fluorescent tube is connected to a power supply, and the lighting simulation control unit is set outside the outermost cultivation sub-unit.
Owner:QINGDAO INST OF MARINE GEOLOGY

Group b streptococcus identification and drug susceptibility testing card, group b streptococcus identification and drug susceptibility testing kit and methods of using the same

The invention discloses an identification and drug sensitivity test card for group B streptococcus, an identification and drug sensitivity test kit for the group B streptococcus and a using method of the identification and drug sensitivity test kit for the group B streptococcus. The identification and drug sensitivity test card for the group B streptococcus comprises a test card body, wherein an identification micropore area and a drug sensitivity test area are arranged on the test card body; the identification micropore area is provided with a plurality of identification micropores; the drug sensitivity test area is provided with a plurality of drug sensitivity micropores. According to the identification and drug sensitivity test card for the group B streptococcus disclosed by the invention, an identification experiment and a drug sensitivity test experiment on the group B streptococcus can be carried out at the same time, and the convenience is better. Based on a display method / a turbidimetric method, identification and drug sensitivity experiments on the group B streptococcus are carried out; compared with nucleic acid test, the test cost is lower.
Owner:广东阳光生物科技有限公司

Group of real-time fluorescent QRT-PCR primers capable of detecting cattle, sheep akabane virus by one-step method, probe and usage method thereof

The invention discloses a group of real-time fluorescent QRT-PCR primers capable of detecting cattle, sheep akabane virus by a one-step method, a probe and a usage method thereof. The nucleotide sequences of the described primers and the probe are respectively as shown in SEQIDNO.1-3. The method for detecting the cattle, sheep akabane virus is as follows: sample RNA is used as template, the above primers and probe are adopted to perform the one-step real-time fluorescent QRT-PCR amplification test of the sample RNA, after reaction, if a specific fluorescent amplification curve appears, the sample RNA is judged to contain the cattle, sheep akabane virus nucleotide, namely the sample is tested positive for the cattle, sheep akabane virus. A standard curved prepared according to analog standards can quantify the one-step real-time fluorescent QRT-PCR amplification test. The method for detecting the cattle, sheep akabane virus is safe, quick, high in specification and sensitivity, and provides technical support for detection of the cattle, sheep akabane disease in livestock husbandry and the inspection and quarantine work of the exit cattle and sheep.
Owner:INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Nucleic acid amplification detection micro-fluidic chip

The invention relates to the field of in-vitro medicine, in particular to a nucleic acid amplification detection micro-fluidic chip which comprises: a micro-fluidic chip; membranes which respectively cover the upper surface and the lower surface of the micro-fluidic chip; a primer chamber which is arranged on the micro-fluidic chip and is used for presetting a primer; a reaction amplification chamber which is arranged on the micro-fluidic chip, is connected with the primer chamber and is used for presetting an amplification reaction mixture; and a detection chamber which is arranged on the micro-fluidic chip, is communicated with the reaction amplification chamber and is used for presetting a fluorescence detection reagent. The nucleic acid amplification detection micro-fluidic chip is sealed by adopting a membrane structure, a sample is not easy to leak, aerosol pollution is not easy to cause, a detection result is not influenced, the sample is convenient to add, the use amount of the sample is small, a PCR (Polymerase Chain Reaction) tube is not needed, the operation is convenient, the chip is suitable for rapid detection, the communication state between the front-end reaction chamber and the rear-end reaction chamber can be accurately controlled, and the whole structure is lighter.
Owner:吉特吉生物技术(苏州)有限公司

Closed type PCR (Polymerase Chain Reaction) amplification tube for integrated gene detection

The invention discloses a closed PCR amplification tube for integrated detection of genes, comprising a tube body for containing PCR buffer, a liquid storage pool for containing hybridization buffer, and a tube with an upper opening of the sealed liquid storage pool Cover, the liquid storage tank is put into the upper part of the tube body, the upper end of the liquid storage tank is airtightly connected with the opening upper end of the tube body, there is a gap between the outer side of the liquid storage tank and the inner wall of the tube body, and the upper part of the liquid storage tank is provided with The through hole that communicates the void with the inside of the liquid storage tank. The invention not only enables PCR, gene hybridization, and gene detection to be completed in a closed system, avoiding cross-contamination, but also completely isolates the PCR amplification system and DNA hybridization system, and avoids components such as buffers and enzymes in the amplification system The impact on the hybridization reaction greatly improves the accuracy and repeatability of genetic testing. The tube cap of the invention makes adding samples easier, has stronger sealing performance, and has better detection accuracy.
Owner:SOUTHEAST UNIV

Liposome High Pressure Extrusion Filtration Device

The invention discloses a high pressure extrusion and filtration device for liposome. The device comprises a bottomless extrusion filter body, the bottomless extrusion filter body is a cavity with the upper portion in a circular-tube shape and the lower portion in a conical shape, a sample feeding opening is formed in the upper end of the bottomless extrusion filter body, the sample feeding opening is movably connected with a screw cap, the device further comprises a filter bottom, and the filter bottom is a cylinder provided with the bottom, wherein the upper end of the filter bottom is open, a discharging pipe is arranged at the bottom of the filter bottom, centripetal protruding blocks are arranged on the inner surface of the side wall of the filter bottom, and a lower supporting network, a filter membrane and an upper supporting network are movably connected to the protruding blocks in sequence. By means of the device, liposome samples can be extruded multiple times, operation time is shortened, and convenience and high efficiency of large-scale liposome preparation in the laboratory are achieved. High efficiency is particularly embodied when the same sample is in need of multiple times of filtration operation. The device is not prone to air leakage under high pressure gas, and therefore reliability of an extrusion filter is improved. The high pressure extrusion and filtration device is simple in structure, and high temperature sterilization can be conducted on the extrusion filter easily.
Owner:INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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