Novel gel electrophoresis unit

Abstract

The invention relates to a novel gel electrophoresis unit which mainly comprises an electrophoresis tank body, a mating electrophoresis tank cover, a gel preparation comb and a gel preparation platform, wherein clamping grooves are formed on the left side and the right side of the gel preparation platform; two dismountable and smooth rectangular rubber plates are fitted with the clamping grooves;a water tank is arranged at the lower end of the gel preparation platform; a water inlet and outlet valve is arranged at the bottom of the water tank; black paint is applied below the gel preparationplatform; the electrophoresis tank body is connected with an electrophoresis buffer collection bottle through a Y guide pipe; the electrophoresis buffer collection bottle is connected with a gas pumpthrough a guide pipe; and a positive electrode wire and a negative electrode wire are arranged at the left end and the right end of the electrophoresis tank cover. A gel pouring tank, a gel loading tank and an electrophoresis tank are designed integrally; contact between a user and carcinogenic dye ethidium bromide (EB) gel is reduced; degradation of nucleic acid in a gel electrophoresis process is effectively avoided by the cooling water tank; a gel electrophoresis step is simplified; gel electrophoresis operation of the user is facilitated; and the electrophoresis efficiency is improved.

Description

technical field [0001] The invention relates to a gel electrophoresis apparatus, which can be used in electrophoresis experiments in research fields such as biochemistry, genetics, system and evolutionary biology. Background technique [0002] Agarose gel electrophoresis is an electrophoresis method that uses agar or agarose as a supporting medium. It is widely used in molecular biology research. The main difference between it and other supports is that it has both "molecular sieve" and "electrophoresis". dual role. The principle is: agarose gel has a network structure, and the material molecules will be resisted when they pass through, and the resistance of the macromolecular substances will be large when they surge. The separation of charged particles depends not only on the nature and quantity of the net charge, but also on the The size of the molecule greatly improves the resolution ability, and is now widely used in the research of nucleic acids, and has become one of ...

AI Technical Summary

Problems solved by technology

The above-mentioned traditional gel electrophoresis instrument, the electrophoresis operation is cumbersome, and in the deoxyribonucleic acid experiment, the cancer mutagen ethidium bromide is used as the dye, which is easy to cause harm to the operator during the gel preparation and transfer process

Secondly, the temperature rise during electrophoresis can easily lead to sample degradation and affect the experimental results.

Therefore, the existing gel electrophoresis instruments are not ideal

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