Nucleic acid amplification detection micro-fluidic chip

Abstract

The invention relates to the field of in-vitro medicine, in particular to a nucleic acid amplification detection micro-fluidic chip which comprises: a micro-fluidic chip; membranes which respectively cover the upper surface and the lower surface of the micro-fluidic chip; a primer chamber which is arranged on the micro-fluidic chip and is used for presetting a primer; a reaction amplification chamber which is arranged on the micro-fluidic chip, is connected with the primer chamber and is used for presetting an amplification reaction mixture; and a detection chamber which is arranged on the micro-fluidic chip, is communicated with the reaction amplification chamber and is used for presetting a fluorescence detection reagent. The nucleic acid amplification detection micro-fluidic chip is sealed by adopting a membrane structure, a sample is not easy to leak, aerosol pollution is not easy to cause, a detection result is not influenced, the sample is convenient to add, the use amount of the sample is small, a PCR (Polymerase Chain Reaction) tube is not needed, the operation is convenient, the chip is suitable for rapid detection, the communication state between the front-end reaction chamber and the rear-end reaction chamber can be accurately controlled, and the whole structure is lighter.

Description

technical field [0001] The invention relates to the technical field of in vitro medical detection, in particular to a nucleic acid amplification detection microfluidic chip. Background technique [0002] Nucleic acid detection is currently widely used in the fields of disease diagnosis, food safety, and environmental testing. The usual nucleic acid detection process is: Weigh the sample, use a kit to extract nucleic acid, then mix the extracted nucleic acid with amplification reagents, and put it into a PCR instrument. Amplification reactions followed by data analysis. The operation process is complicated, and it is easy to cause human error when testing multiple samples and multiple indicators, which is time-consuming and inefficient. [0003] Microfluidic chip is an emerging technology for manipulating and detecting chemical or biological samples on a chip. The microfluidic chip can integrate the operation units of sample preparation, reaction, separation and detection i...

AI Technical Summary

Problems solved by technology

However, the existing microfluidic chips cannot accurately control the communication state between the front-end and the back-end reaction chambers during detection, and the structure is mostly hard shells that are interlocked with each other, which is not conducive to light weight and needs further improvement

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