Automatic detection system of genotyping chip

Abstract

The invention relates to the field of in-vitro diagnostic reagent detection, in particular to an automatic detection system of a genotyping chip. The detection system comprises a hybridization temperature control assembly, a pipetting assembly, a moving guide rail and a hybridization cleaning assembly, the hybridization temperature control assembly comprises a genotyping chip container, and a genotyping chip film strip can be horizontally placed in the genotyping chip container; the pipetting assembly comprises a needle discarding window, a needle turntable, a PCR product middle turntable andan image acquisition device which are sequentially arranged in the same straight line direction; the hybridization cleaning assembly comprises a liquid adding mechanism and a hybridization cleaning mechanism. The detection system has the beneficial effect that rapid and convenient suction and sample adding can be realized. According to the automatic detection system of the genotyping chip, automatic hybridization and detection are achieved, continuity of detection steps is guaranteed, the workload of manual intervention is small, and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the field of detection of in vitro diagnostic reagents, in particular to an automatic detection system of a genotyping chip. Background technique [0002] At present, the main methods for clinical DNA detection in China are real-time fluorescent PCR and hybrid capture method. The PCR-membrane hybridization method adopts gene amplification technology and diversion hybridization principle, and detects the amplified product and coating type specificity by reverse dot hybridization. The result of membrane hybridization of the probe is then qualitatively detected by the alkaline phosphatase system, so as to carry out genotyping detection and analysis, which has important clinical application value for preventing the occurrence of diseases. [0003] At present, the genotyping chip of PCR-reverse dot hybridization method usually adopts the method of sample extraction-PCR amplification-constant temperature hybridization-visible light i...

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Problems solved by technology

[0003] At present, the genotyping chip of PCR-reverse dot hybridization method usually adopts the method of sample extraction-PCR amplification-constant temperature hybridization-visible light interpretation, and the workload of manual intervention is relatively large.

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