Closed type PCR (Polymerase Chain Reaction) amplification tube for integrated gene detection

Abstract

The invention discloses a closed PCR amplification tube for integrated detection of genes, comprising a tube body for containing PCR buffer, a liquid storage pool for containing hybridization buffer, and a tube with an upper opening of the sealed liquid storage pool Cover, the liquid storage tank is put into the upper part of the tube body, the upper end of the liquid storage tank is airtightly connected with the opening upper end of the tube body, there is a gap between the outer side of the liquid storage tank and the inner wall of the tube body, and the upper part of the liquid storage tank is provided with The through hole that communicates the void with the inside of the liquid storage tank. The invention not only enables PCR, gene hybridization, and gene detection to be completed in a closed system, avoiding cross-contamination, but also completely isolates the PCR amplification system and DNA hybridization system, and avoids components such as buffers and enzymes in the amplification system The impact on the hybridization reaction greatly improves the accuracy and repeatability of genetic testing. The tube cap of the invention makes adding samples easier, has stronger sealing performance, and has better detection accuracy.

Description

technical field [0001] The invention relates to a closed PCR amplification tube, which can be used for integrated detection of gene information, that is, corresponding functions such as gene amplification, hybridization, and detection are sequentially completed in the same closed space. Background technique [0002] At present, polymerase chain reaction is one of the indispensable technologies in various detection and research processes involving nucleic acid molecules, such as molecular biology, gene detection, disease detection and diagnosis. As we all know, the PCR (Polymerase Chain Reaction, Polymerase Chain Reaction) amplification method is extremely sensitive, and can amplify the gene to be tested at a low concentration to tens of thousands to millions of times. detected by electrophoresis. Because of the high sensitivity of PCR amplification, cross-contamination is prone to occur during the amplification process, and the contamination is easily amplified, resulting i...

AI Technical Summary

Problems solved by technology

Although the gene chip can be used to obtain high-throughput gene information and detect multiple genes at the same time, the amplification of the gene to be tested, the transfer of the amplified product, and the hybridization are all completed in different systems. It will inevitably increase the possibility of contamination, thereby reducing the reliability of experimental detection

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