Gene-detection integrally completed enclosed PCR (polymerase chain reaction) amplification tube

Abstract

The invention discloses a gene-detection integrally completed enclosed PCR (polymerase chain reaction) amplification tube which comprises a tube body, a solution storage tank and a tube cover, wherein the tube body is used for accommodating a PCR buffer solution, the solution storage tank is used for accommodating a hybridized buffer solution, the tube cover encloses an opening on the upper side of the solution storage tank, the solution storage tank is placed at the upper part of the tube body, the upper end of the solution storage tank is hermetically connected with the upper end of the opening of the tube body, the bottom surface of the solution storage tank is provided with a communicating tube for communicating the upper and lower sides of the bottom surface, and the lower end of the communicating tube is connected with the bottom surface. According to the invention, PCR, gene hybridization and gene detection can be completed in an enclosed system, thereby avoiding cross contamination; and PCR amplification system is completely isolated from a DNA hybridization system, thereby avoiding the influence of compositions such as buffer solutions and enzyme and the like in the amplification system on hybridization reaction, and greatly improving the accuracy and repeatability of gene detection. Due to the tube cover of the enclosed PCR amplification tube disclosed by the invention, the sample adding is more convenient, the tightness is strong, and the detection accuracy is better.

Description

technical field [0001] The invention relates to a closed PCR amplification tube, which can be used for integrated detection of gene information, that is, corresponding functions such as gene amplification, hybridization, and detection are sequentially completed in the same closed space. Background technique [0002] At present, polymerase chain reaction is one of the indispensable technologies in various detection and research processes involving nucleic acid molecules, such as molecular biology, gene detection, disease detection and diagnosis. As we all know, PCR (Polymerase Chain Reaction, Polymerase Chain Reaction), the amplification method is extremely sensitive, and can amplify the gene to be tested at a low concentration to tens of thousands to millions of times. It can be detected by electrophoresis. Because of the high sensitivity of PCR amplification, cross-contamination is prone to occur during the amplification process, and the contamination is easily amplified, ...

AI Technical Summary

Problems solved by technology

Although the gene chip can be used to obtain high-throughput gene information and detect multiple genes at the same time, the amplification of the gene to be tested, the transfer of the amplified product, and the hybridization are all completed in different systems. It will inevitably increase the possibility of contamination, thereby reducing the reliability of experimental detection

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