Group of real-time fluorescent QRT-PCR primers capable of detecting cattle, sheep akabane virus by one-step method, probe and usage method thereof

A technology of Akabane disease virus and probe, which is applied in the field of sheep Akabane disease virus and cattle detection, can solve the problems of insufficient sensitivity of cattle and sheep Akabane disease virus methods, achieve good promotion and application value, ensure safety, and solve sensitivity problems Effect
CN103789455BActive Publication Date: 2015-04-22INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2015-04-22

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Abstract

The invention discloses a group of real-time fluorescent QRT-PCR primers capable of detecting cattle, sheep akabane virus by a one-step method, a probe and a usage method thereof. The nucleotide sequences of the described primers and the probe are respectively as shown in SEQIDNO.1-3. The method for detecting the cattle, sheep akabane virus is as follows: sample RNA is used as template, the above primers and probe are adopted to perform the one-step real-time fluorescent QRT-PCR amplification test of the sample RNA, after reaction, if a specific fluorescent amplification curve appears, the sample RNA is judged to contain the cattle, sheep akabane virus nucleotide, namely the sample is tested positive for the cattle, sheep akabane virus. A standard curved prepared according to analog standards can quantify the one-step real-time fluorescent QRT-PCR amplification test. The method for detecting the cattle, sheep akabane virus is safe, quick, high in specification and sensitivity, and provides technical support for detection of the cattle, sheep akabane disease in livestock husbandry and the inspection and quarantine work of the exit cattle and sheep.
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Description

technical field

[0001] The invention belongs to the technical field of virus molecular biology detection. More specifically, it relates to a set of one-step real-time fluorescent QRT-PCR primers, probes and usage methods for detecting bovine and sheep Akabane disease viruses. Background technique

[0002] Akabane disease (AKAD), also known as Akabane spot disease, is a polymorphic viral infectious disease of cattle, sheep and goats caused by Akabane virus (AKAV). Symptoms of the disease mainly include miscarriage, premature birth, stillbirth, fetal malformation, mummification, etc., which can cause congenital arthrogryposis and hydrocephalus syndrome. The disease is an insect-borne infectious disease, and the vectors are blood-sucking mosquitoes and Culicoides. Akabane disease is a legally notifiable disease of the World Organization for Animal Health (OIE), and it is also one of the seven diseases that must be tested for cattle and sheep imported from abroad in my country...

Claims

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