Detection kit and detection method for porcine circovirus type 2 antibody

A porcine circovirus and detection kit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long detection time, difficulty in quantification, and difficulty in automation, and achieve short reaction time, improved repeatability, and wide detection range Effect

Active Publication Date: 2018-07-20
上海鸣捷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems of long detection time, difficulty in quantification, poor repeatability, and difficulty in automation in the existing veterinary detection technology, the purpose of the present invention is to provide a detection kit for porcine circovirus type 2 antibody, which can quantitatively detect porcine circovirus type 2 antibody Circovirus type 2 antibody content

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  • Detection kit and detection method for porcine circovirus type 2 antibody
  • Detection kit and detection method for porcine circovirus type 2 antibody
  • Detection kit and detection method for porcine circovirus type 2 antibody

Examples

Experimental program
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Effect test

Embodiment 1

[0098] A kit for detecting porcine circovirus type 2 antibody, comprising magnetic suspension coated with porcine circovirus type 2 Cap protein, alkaline phosphatase-labeled porcine circovirus type 2 antibody solution, diluent, calibrator, Quality control products, washing solutions, luminescent substrates.

[0099] Preparation of magnetic suspension coated with porcine circovirus type 2 Cap protein:

[0100] (1) Take 1 mL of magnetic particles (purchased from JSR Corporation, Japan, item number: Magnosphere TM MS300 / Caboxyl) solution with a concentration of 10 mg / mL, washed twice with 0.1 mol / L 2-morpholineethanesulfonic acid (MES) buffer solution with a pH of 5.0, and finally suspended in 1 mL of 0.1 mol / L pH 5.0 In MES buffer;

[0101] (2) 70 μg of purified porcine circovirus type 2 Cap protein (purchased from Qingdao Yibang Bioengineering Co., Ltd.) was added;

[0102] (3) Weigh 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), dissolve it with 0.1mol / L MES buffer s...

Embodiment 2

[0164] A kit for porcine circovirus type 2 antibody detection, comprising a magnetic suspension coated with porcine circovirus type 2 Cap protein, and acridinium ester-labeled goat anti-pig IgG antibody (purchased from Beijing Suo Laibao Technology Co., Ltd.) Solutions, diluents, calibrators, quality controls, wash solutions, primary luminescent substrates and secondary luminescent substrates.

[0165] Preparation of magnetic suspension coated with porcine circovirus type 2 Cap protein:

[0166] (1) Take 1mL of the solution containing magnetic particles with a concentration of 10mg / mL, wash it twice with 0.1mol / L boric acid buffer solution with a pH of 9.5, and finally suspend in 1mL of a 0.1mol / L boric acid buffer solution with a pH of 9.5;

[0167] (2) Add 80 μg of purified porcine circovirus type 2 Cap antigen, vortex and mix;

[0168] (3) Add 0.5 mL of 0.1 mol / L boric acid buffer (containing 3 mol / L ammonium sulfate) with a pH of 9.5, and shake and react at 37°C for 20 ho...

Embodiment 3

[0212] A test kit for porcine circovirus type 2 antibody detection, comprising a magnetic suspension coated with porcine circovirus type 2 Cap protein, horseradish peroxidase-labeled porcine circovirus type 2 Cap antigen solution, diluent, Calibrator, Quality Control, Washing Solution, Primary Luminescent Substrate and Second Luminescent Substrate.

[0213] Preparation of magnetic suspension coated with porcine circovirus type 2 Cap protein:

[0214] (1) Take 1mL of the solution containing magnetic particles, the concentration is 10mg / mL, wash twice with 0.01mol / L phosphate (PBS) buffer solution with pH 7.4, and finally suspend in 1mL 0.01mol / L PBS with pH 7.4 in the buffer;

[0215] (2) Add 0.1 mL of 25% (v / v) glutaraldehyde solution, shake and react at 37°C for 2 hours;

[0216] (3) Wash 3 times with 1mL 0.01mol / L PBS buffer solution with pH 7.4;

[0217] (4) Adding 30 μg of purified porcine circovirus type 2 Cap recombinant antigen, shaking and reacting at 37°C for 20 ho...

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Abstract

The invention discloses a detection kit and a detection method for a porcine circovirus type 2 antibody. The kit detects the content of the antibody in a sample to be detected through a specific reaction of a magnetic particle-coated antigen, a luminescent marker-labeled protein and the antibody in the sample to be detected. The kit realizes the quantitative detection of the porcine circovirus type 2 antibody, and has the advantages of high sensitivity, wide detection range, fastness, good repeatability, and realization of fully automatic operation and high-throughput detection.

Description

technical field [0001] The invention relates to the technical field of porcine circovirus type 2 antibody detection, in particular to a porcine circovirus type 2 antibody detection kit and a detection method using the kit for detection. Background technique [0002] Porcine circovirus type 2 (Porcine circovirus type 2, PCV2) is the smallest animal virus found so far. The diameter of the virus particle is about 17nm. It is a covalently closed, circular, single-stranded DNA virus. PCV has two genotypes: PCV1 and PCV2. PCV1 is non-pathogenic, but widely exists in pigs and pig-derived cell lines. PCV2 is pathogenic to pigs and mainly causes post-weaning multisystemic wasting syndrome (PMWS) in weaned piglets. In addition to causing PMWS, PCV2 is also associated with congenital tremor in piglets, porcine dermatitis nephritic syndrome, porcine respiratory syndrome (PDNS), and reproductive disorders in sows. Since it was first reported in Canada in 1991, many countries and region...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/543G01N33/535
CPCG01N33/535G01N33/54326G01N33/56983G01N33/577Y02A50/30
Inventor 左青山宋启超纪良心刘聪李炎晖
Owner 上海鸣捷生物科技有限公司
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