One-stop reactor and nucleic acid detection analyser

A reactor and station technology, applied in the field of one-stop reactors and nucleic acid detection analyzers, can solve the problems of large size of the kit, complex internal structure, low heating rate, etc., to avoid nucleic acid transfer and reduce operation steps. , the effect of avoiding pollution

Inactive Publication Date: 2021-07-23
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a one-stop reactor and nucleic acid detection analyzer to solve the problems of large volume, complex internal structure, low heating rate, long detection time, large loss during nucleic acid extraction, and easy contamination of the kit.

Method used

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  • One-stop reactor and nucleic acid detection analyser
  • One-stop reactor and nucleic acid detection analyser

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] like figure 1 , figure 2 As shown, one-stop reactor, including a cover 51 and a reaction tube 52,

[0051] The grease hole 51 is opened, and the reagent storage tube 54 is mounted in the plug-in, and the second reagent is mounted in the reagent storage tube, and the cover 51 has a tube hole and a tester hole. Sealed film;

[0052] The reaction tube 52 has a reaction cavity 53;

[0053] The cover 51 and the reaction tube 52 are mounted to connect the reaction cavity in a sealing state.

[0054] The cross section of the cover 51 is circular or square. Cylindrical is convenient.

[0055] A first protrusion 56 is provided on the outer wall of the cover. The first protrusion is used to fit the installation hole position of the instrument to position a one-stop reactor.

[0056] A rubber sealing plug 55 is mounted in the plug-in hole.

[0057] A second projection is provided on the inner wall of the plug-in hole, and the rubber sealing plug 55 has a second groove adapted to the s...

Embodiment 2

[0096] One-stop reactor, the inside of one-stop reactor has a reactive cavity, and a plurality of sample holes and plus tester holes are opened on one-stop reactor; reagent storage tube 54, reagent storage tube There is a second reagent; a sealing film having a sealing and sample hole and a tester hole in one-stop reactor.

[0097] A first protrusion 56 is provided on the outer wall of one-stop reactor. The first protrusion is used to fit the installation hole position of the instrument to position a one-stop reactor.

[0098] A rubber sealing plug 55 is mounted in the plug-in hole.

[0099] A second projection is provided on the inner wall of the plug-in hole, and the rubber sealing plug 55 has a second groove adapted to the second projection.

[0100] The reagent storage tube 54 is actively mounted in the dosage hole.

[0101] The reagent storage tube 54 slides in the dosing agent hole of the tube cover 51.

[0102] The reagent storage tube is a tubular shape, and the reagent st...

Embodiment 3

[0115] A nucleic acid detection analyzer, including a temperature control module, a detection module, further comprising a one-stop reactor, a temperature control module controlling a temperature in the reaction chamber of the one-stop reactor, a detection module to one-stop reactor The reaction liquid in the reaction chamber was detected.

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Abstract

The invention relates to a nucleic acid detection technology, in particular to a one-stop reactor and a nucleic acid detection analyser. The one-stop reactor comprises a pipe cover and a reaction pipe, wherein the pipe cover is provided with a sample adding hole and a reagent adding hole; a reagent storage pipe is arranged in the reagent adding hole and is filled with a second reagent, and a sealing film for sealing the sample adding hole and the reagent adding hole is arranged on the pipe cover; and a reaction cavity is formed in the reaction pipe, the pipe cover and the reaction pipe are matched and connected to enable the reaction cavity to be in a sealed state, and openings at two ends of the reagent adding hole are provided with sealing films. According to the one-stop reactor and the nucleic acid detection analyser, the problems that a kit is large in size, complex in internal structure, low in heating rate, long in detection time, large in loss in the nucleic acid extraction process, prone to be polluted and the like are solved.

Description

Technical field [0001] The present invention relates to nucleic acid detection techniques, and more particularly to one-stop reactor and a nucleic acid detection analyzer. Background technique [0002] Nucleic acid detection techniques are a method of detecting molecular biology, requiring nucleic acid fragments (DNA or RNA) from the sample, and then replicates the nucleic acid fragment by polymerase chain reaction, and finally detects nucleic acids. Determine the specific category or source of nucleic acid in the sample. [0003] The nucleic acid detection method is simple and easy, the application is extremely extensive, and only a small amount of sample can get a precise result. Sampling first needs to be sampled, usually use a swab to collect samples into the sampling tube, and then extract from the sampling tube to the kit, and finally put into the instrument. [0004] During nucleic acid extraction and amplification, it is necessary to add a first reagent, a second reagent,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12M1/34C12M1/02C12M1/00
CPCC12Q1/6848B01L3/50C12Q2531/113C12Q2547/101
Inventor 汪云飞潘能科
Owner ZYBIO INC
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