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Liposome High Pressure Extrusion Filtration Device

A filter device and body high pressure technology, applied in the field of biomedicine, can solve problems such as difficulty in meeting laboratory preparation requirements, insufficient extrusion of liposome samples, cumbersome multiple continuous extrusion operations, etc., to achieve unique convenience and Effectiveness, ease of high-temperature sterilization, convenience and high efficiency

Active Publication Date: 2018-03-06
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sample processing volume of the former is mostly 0.1mL-1mL, using manual thrust extrusion, the disadvantage is that the processing volume is too small, it is difficult to meet the needs of laboratory preparation, and manual thrust operation is more laborious, liposome sample extrusion is not sufficient
The latter equipment is cumbersome and inconvenient to operate when liposomes are extruded once, and it is particularly cumbersome when extruding liposomes in multiple consecutive operations

Method used

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  • Liposome High Pressure Extrusion Filtration Device
  • Liposome High Pressure Extrusion Filtration Device
  • Liposome High Pressure Extrusion Filtration Device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Take 60 mg of dry phospholipids and 9 mg of cholesterol, dissolve them in 10 mL of chloroform, and then rotate to evaporate to form a lipid film, add 5 mL of phosphate buffer (pH 7.4) and shake by hand to form liposomes with different particle sizes. At 3-30μm (see image 3 ). Afterwards, the liposome suspension was added to the liposome high-pressure extrusion filtration device of the present invention, and after 10 times of extrusion and filtration, a liposome suspension with a particle size of about 1 μm and a relatively uniform particle size distribution was obtained (see Figure 4 ).

Embodiment 2

[0042] The liposomes are high-pressured out of the various components of the filter device (except the filter device holder 11 and the clip 12), autoclaved in an autoclave, taken out and dried, and then assembled in a sterile environment. Then, the viscous collagen swelling solution is added to the extrusion filtration device, and the sterile collagen swelling solution can be obtained after squeezing and filtering (the filter membrane is a polyethersulfone filter with a pore size of 0.22 μm).

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Abstract

The invention discloses a high pressure extrusion and filtration device for liposome. The device comprises a bottomless extrusion filter body, the bottomless extrusion filter body is a cavity with the upper portion in a circular-tube shape and the lower portion in a conical shape, a sample feeding opening is formed in the upper end of the bottomless extrusion filter body, the sample feeding opening is movably connected with a screw cap, the device further comprises a filter bottom, and the filter bottom is a cylinder provided with the bottom, wherein the upper end of the filter bottom is open, a discharging pipe is arranged at the bottom of the filter bottom, centripetal protruding blocks are arranged on the inner surface of the side wall of the filter bottom, and a lower supporting network, a filter membrane and an upper supporting network are movably connected to the protruding blocks in sequence. By means of the device, liposome samples can be extruded multiple times, operation time is shortened, and convenience and high efficiency of large-scale liposome preparation in the laboratory are achieved. High efficiency is particularly embodied when the same sample is in need of multiple times of filtration operation. The device is not prone to air leakage under high pressure gas, and therefore reliability of an extrusion filter is improved. The high pressure extrusion and filtration device is simple in structure, and high temperature sterilization can be conducted on the extrusion filter easily.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a liposome high-pressure extrusion filtration device. Background technique [0002] Liposomes are closed vesicles similar to cell membrane structure formed by self-assembly of phospholipid molecules dispersed in the water phase, with a particle size ranging from tens of nanometers to several micrometers. It was first discovered by Bangham in 1965, and then named liposomes. Liposomes can be divided into multilamellar liposomes (MLV), large unilamellar liposomes (LUV) and small unilamellar liposomes (SUV) according to the number of layers of lipid bilayers. Liposomes can be used as carriers for traditional small-molecule chemical drugs or biomacromolecule drugs such as proteins, vaccines, DNA, and SiRNA. They are a type of targeted drug delivery system. [0003] Liposomes are one of the most popular new dosage forms for drug delivery and one of the more mature drug dosage ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D61/00A61L2/02
CPCA61L2/022B01D61/00B01D2311/14
Inventor 陈汉秦迎州刘玲蓉李学敏张明明关嫚
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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