Closed QPCR reaction vessel

Abstract

The invention provides a closed QPCR reaction container. The reaction container at least comprises a shell, a cover body and a reaction area; the shell and the cover body are mutually covered to forma reaction area; the reaction area comprises a reagent tube rack, and when the reagent tube rack is full of reagent tubes, the reaction area is closed; and the upper top surface of the cover body is provided with a telescopic structure area, the area of the telescopic structure area covers the whole reaction area, the telescopic structure area is provided with a pipette tip seat communicating withthe interior of a closed cavity, and the pipette tip seat can do planar movement above the reaction area through the telescopic structure area. According to the closed QPCR reaction container, closedoperation of the whole process from liquid pretreatment to QPCR reaction completion can be achieved, rapidness and high efficiency are achieved, and experiment errors caused by manual operation can be prevented. The closed QPCR reaction container is small in size and small in occupied space, is suitable for portable equipment, has the advantages of good impact resistance and low cost, solves thepollution problem, and is very suitable for tumor detection.

Description

technical field [0001] The invention relates to the field of biological detection instruments, in particular to a closed QPCR reaction container. Background technique [0002] QPCR: (Quantitative Real-time PCR) is a method of measuring the total amount of products after each cycle of polymerase chain reaction (PCR) with a fluorescent chemical substance in a DNA amplification reaction. A method for quantitative analysis of a specific DNA sequence in a sample to be tested by using an internal or external reference method. According to the basic principles of molecular diagnosis, many automated detection platforms have been developed at home and abroad, such as nucleic acid extraction instrument, nucleic acid hybridization instrument, chemiluminescence instrument, PCR instrument, etc. However, most of the existing platforms in the domestic market are based on a single technology, and the equipment is large in size. It can only complete a certain step in the molecular diagnosis...

AI Technical Summary

Problems solved by technology

These manual operations are not only cumbersome, but also time-consuming and labor-intensive. The differences in the proficiency of the operators lead to large differences in the experimental results.

In addition, because PCR experiments are extremely susceptible to aerosol pollution, and the current anti-pollution measures for platforms in the domestic market are not satisfactory

At the same time, the existing products use the prototype chassis for the sampling operation, which not only makes it difficult to accurately control the accuracy, but also has the disadvantages of easy box jamming and insufficient airtightness

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