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Application of 2-o-mercapto-acetic indican ascorbic acid in preparation of semen for controlling gender

A technology of ascorbic acid and glucoside, applied in the application field of 2-O-glucoside ascorbic acid in the preparation of sex control semen, to avoid damage and improve sperm motility

Inactive Publication Date: 2011-02-16
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are no reports on the application of AA-2G in the preparation of sex-controlled semen

Method used

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  • Application of 2-o-mercapto-acetic indican ascorbic acid in preparation of semen for controlling gender
  • Application of 2-o-mercapto-acetic indican ascorbic acid in preparation of semen for controlling gender
  • Application of 2-o-mercapto-acetic indican ascorbic acid in preparation of semen for controlling gender

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Take a certain amount of raw pig semen and dilute it with Hepes buffer to 200×10 6 Sperm / mL, then add a final concentration of 0.11mM H33342 and a final concentration of 200μM AA-2G (0.068mg / ml), and stain in a 34°C water bath for 45min;

[0020] 2. Further dilute to 100×10 with Hepes buffer containing 4% (g / ml) purified yolk and 5% food red (g / ml) 6 Sperm / mL;

[0021] 3. Then use the SX-MoFlo flow cytometer to separate and recover the sperm, and detect the collected single-sex sperm to obtain the porcine sex-controlled semen.

Embodiment 2

[0023] 1. Take a certain amount of raw pig semen and dilute it with Hepes buffer to 200×10 6 Sperm / mL, then add a final concentration of 0.11mM H33342 and a final concentration of 250μM AA-2G (0.085mg / ml), stain in a 34°C water bath for 45min;

[0024] 2. Further dilute to 100×10 with Hepes buffer containing 4% (g / ml) purified yolk and 5% food red (g / ml) 6 Sperm / mL;

[0025] 3. Then use the SX-MoFlo flow cytometer to separate and recover the sperm, and detect the collected single-sex sperm to obtain the porcine sex-controlled semen.

[0026] The prepared porcine sex control semen was detected by fluorescence microscope, and the results showed that the acrosome integrity and plasma membrane integrity of the sperm were good.

Embodiment 3

[0028] The method was the same as in Example 1, except that the final concentration of AA-2G was adjusted to 150 μM (0.051 mg / ml) to obtain porcine sex-controlled semen.

[0029] The prepared porcine sex control semen was detected by fluorescence microscope, and the results showed that the acrosome integrity and plasma membrane integrity of the sperm were good.

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Abstract

The invention provides application of 2-o-mercapto-acetic indican ascorbic acid in preparation of semen for controlling gender, which has the following advantages that AA-2G is applied to a preparation process of the semen for controlling gender to inhibit a large amount of free oxygen generated in the preparation process; and because the AA-2G is stable in water solution, the damage to the semen caused by treatment processes of separation, centrifugation and the like in the preparation process can be avoided to the maximum, thereby obviously improving semen quality parameters, such as semen activity, acrosome completeness and plasmalemma completeness, and the 2-o-mercapto-acetic indican ascorbic acid is obviously prior to the traditional anti-oxidizing agent with respect to action effect and action time.

Description

technical field [0001] The present invention relates to a new application of 2-O-glucoside ascorbic acid, in particular to the application of 2-O-glucoside ascorbic acid in preparing sex control semen. Background technique [0002] 2-O-glucoside ascorbic acid (AA-2G) is a derivative of ascorbic acid (VC), which is formed by substituting the hydroxyl group at the 2-position of ascorbic acid with a glucosyl group. Since the 2-position is covered by a glucose group, AA-2G is not easily oxidized and is particularly stable in aqueous solution; and AA-2G has no direct reduction, but it can quickly release highly active VC, VC under the action of enzymes Can quickly react with free radicals to reduce lipid peroxidation. [0003] At present, AA-2G is widely used as an antioxidant in many fields such as cosmetics, quasi-drugs and food, and it is also used in the cryopreservation of livestock sperm in the field of reproduction. [0004] Sex control (sex control) technology, that is,...

Claims

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Application Information

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IPC IPC(8): A01K67/02A01N1/02
Inventor 田见晖夏春梅扬升
Owner CHINA AGRI UNIV
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