Western blot kit for syphilis specific total antibodies and anticardiolipin antibodies and preparation method of kit

Abstract

The invention relates to a kit, in particular to a western blot kit for syphilis specific total antibodies and anticardiolipin antibodies and a preparation method of the kit. The kit is provided with a carrier plate, a nitrocellulose membrane, detection lines for syphilis specific total antibodies and anticardiolipin antibodies and a comparison line, wherein the detection lines and the comparison line are arranged on the nitrocellulose membrane; syphilis specific recombinant antigens and anticardiolipin antigens are coated at the detection lines for the total antibodies and anticardiolipin antibodies; anti-human Ig (immunoglobulin G) antibodies are coated at the comparison line; and horse radish peroxidase is labeled with the anti-human Ig antibodies. The method comprises the following steps: preparing the syphilis specific recombinant antigens; preparing the anticardiolipin antigens; applying samples of the nitrocellulose membrane; preparing anti-human total specific segment Ig chain monoclonal antibodies; labeling the horse radish peroxidase with the anti-human Ig monoclonal antibodies; and preparing the western blot kit. The western blot kit can be used for detecting the syphilis specific total antibodies and the anticardiolipin antibodies in the specimens of whole blood, serum, blood plasma, cerebrospinal fluid and the like. The invention has the advantages of small required specimen quantity, strong specificity, high sensitivity and is simple and fast in detection, and accurate and reliable in detection, and special instruments are not needed.

Description

Syphilis-specific total antibody and cardiolipin antibody immunoblotting kit and its preparation technical field The invention relates to a kit, in particular to a syphilis-specific total antibody and cardiolipin antibody immunoblotting kit and a preparation method thereof. Background technique Syphilis is a sexually transmitted disease caused by Treponema pallidum (TP), and its pathogen is Treponema pallidum, which belongs to the family Treponemaceae. Treponema pallidum is mainly transmitted through sexual contact, blood transfusion, wound or placenta. Treponema pallidum enters the blood from the lymph nodes near the infected area, spreads throughout the body, and affects almost all tissues and organs of the body. The World Health Organization (WHO) once predicted optimistically: "Due to the availability of highly sensitive detection methods and efficient treatment options, syphilis is a sexually transmitted disease that can be successfully controlled through public heal...

AI Technical Summary

Problems solved by technology

The commercially available Western-blot reagents are all imported reagents, which are expensive

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