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Method for improving inoculation induction rate of rice blast

A technology for rice blast and rice blast fungus, applied in the field of increasing the inoculation induction rate of rice blast fungus, can solve the problems of low induction rate of artificial inoculation and difficulty in germination of blast fungus, so as to improve hydrophilicity and prolong retention time , the effect of strong water retention

Inactive Publication Date: 2012-05-30
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for inducing rice blast disease in the existing technology that cannot fully meet the requirements of rice blast fungus on high humidity conditions, resulting in difficulty in germination of rice blast fungus and low induction rate of artificial inoculation. The method is convenient to use, can fully meet the requirements of rice blast fungus on high humidity conditions, and improves the inoculation induction rate of rice blast

Method used

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  • Method for improving inoculation induction rate of rice blast
  • Method for improving inoculation induction rate of rice blast
  • Method for improving inoculation induction rate of rice blast

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1: (a kind of method 1 that improves rice blast inoculation induction rate)

[0020] (1) Collection of rice blast fungus conidia: select rice blast fungus strains that are highly pathogenic to rice and produce more conidia through barley grain culture medium; The conidia are washed with water from the barley grains to obtain the spore liquid, and vacuum-filtered through filter paper to obtain filter paper with conidia attached to the surface, which is quickly air-dried for subsequent use;

[0021] (2) Preparation of rice blast fungus inoculum: first by weight percentage, 0.05% hyaluronic acid and 0.025% sucrose lipid are added to 100% with sterile water and mixed into base liquid; then step (1 ) The air-dried filter paper with spores attached to the surface layer was cut into pieces, put into the base solution in batches, stirred sufficiently to make the spores on the filter paper fall off into the base solution, and the conidia content in the solution was me...

Embodiment 2

[0024] Embodiment 2: (a kind of method 2 that improves rice blast inoculation induction rate)

[0025] In this example, the preparation of step (2) rice blast fungus inoculum: by weight percentage, 0.1% polywater is kept and 0.1% XB804, and make up to 100% with sterile water and mix into base liquid; All the other steps Same as embodiment 1 with technology.

Embodiment 3

[0026] Embodiment 3: (a kind of method 3 that improves rice blast inoculation induction rate)

[0027] In this example, the preparation of step (2) rice blast fungus inoculum: 5% glycerol and 1% polysorbate by weight percentage, and make up to 100% with sterile water and mix into base liquid; Step and technology are the same as embodiment 1.

[0028] In order to clarify the beneficial effect of the rice blast fungus inoculum of the present invention, the applicant carried out the following tests. Test example 1: (the impact test of water-retaining agent and surfactant on the germination rate of blast fungus conidia)

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Abstract

The invention discloses a method for improving inoculation induction rate of rice blast, which belongs to the technical field of agricultural plant protection. The method comprises the following steps: firstly, collecting conidiospores of rice blast fungi; secondly, preparing inoculation liquid of rice blast fungi; thirdly, spraying the inoculation liquid for inoculation; and fourthly, observing,recording, counting and the like. In the invention, water retention agent and surface-active agent are added into the inoculation liquid, so that water in the inoculation liquid can be kept effectively; moreover, the hydrophilcity and the spreading property of the surface of a rice plant are improved, so that not only is the amount that the rice plant accepts the inoculation liquid improved, but also the time that the inoculation liquid stays on the rice acceptor is prolonged, further, favorable conditions are provided for induction of rice blast fungi, and the induction rate of artificial inoculation of rice blast is improved remarkably. The method can be popularized and applied to the study of rice blast as well as resistance identification and resistance breeding of rice blast.

Description

technical field [0001] The invention relates to the technical field of plant diseases, in particular to a method for increasing the induction rate of rice blast fungus inoculation. Background technique [0002] The preparation of plant pathogenic bacteria inoculum is one of the important steps in the experiment of inducing plant diseases. Generally, the inoculum used to induce plant disease pathogenic bacteria is an aqueous suspension of the pathogenic bacteria, such as an aqueous suspension of spores or an aqueous suspension of mycelial fragments. Considering the surface characteristics of the inoculated plants, sometimes surfactants, such as Tween 20 and Tween 80, are added to the inoculum to increase the affinity between the inoculum and the plants. [0003] Similar to the above method, the inoculum used for the induction of rice blast is generally an aqueous suspension of the rice blast fungus, and sometimes Tween is added to make the disease conditions more favorable, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00A01G7/06A01G16/00A01G22/22
Inventor 陶荣祥王连平郝中娜梁建根
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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