Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same

Abstract

The invention discloses a nucleic acid hybridization instrument comprising a reagent bottle, a liquid inlet device, a liquid-transferring device, a vibrating trough device and a control system, wherein the liquid inlet device is connected with the liquid-transferring device and provided with a micropump and a membrane pump, and the micropump and the membrane pump are used for driving reagents to flow into the liquid-transferring device from the reagent bottle; the liquid-transferring device is arranged parallel to the vibrating trough device and provided with a needle tube device, the vibrating trough is provided with a hybridization trough, and the needle tube device is used for adding the reagents into the corresponding hybridization trough for hybridization; and the control system is connected with the liquid inlet device, the liquid-transferring device and the vibrating trough device through a controller. The nucleic acid hybridization instrument can carry out full-automatic experiments so as to save a great deal of time, and adopts the liquid inlet device and the liquid-transferring device for sucking and feeding liquid so as to achieve high speed and convenient operation; the vibrating trough device vibrates automatically, and a heating plate is used for carrying out constant temperature heating so that heating can be more direct and fast; and the control device is used for controlling operation so as to improve the accuracy of the experiment and reduce experimental errors.

Description

technical field [0001] The invention relates to a nucleic acid hybridization method and a nucleic acid hybridization instrument using the method. Background technique [0002] At present, the general nucleic acid hybridization method is to manually absorb and add liquid for nucleic acid hybridization, and the mixed liquid is heated in a water bath and vibrated and mixed in a water bath. In this way, nucleic acid hybridization is performed. The accuracy of the liquid volume is difficult to guarantee, and manual operation takes a lot of time, especially when multiple reagents are required, and the heating time of the water bath is long, which greatly prolongs the entire experiment time. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a fully automatic and convenient nucleic acid hybridization method and a nucleic acid hybridization instrument using the method. [0004] In order to solve the above-mentioned technic...

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Problems solved by technology

[0002] At present, the general nucleic acid hybridization method is to manually absorb and add liquid for nucleic acid hybridization, and the mixed liquid is heated in a water bath and vibrated and mixed in a water bath. In this way, nucleic acid hybridization is performed. The accuracy of the liquid volume is difficult to guarantee, and manual operation takes a lot of time, especially when multiple reagents are required, and the heating time of the water bath is long, which greatly prolongs the entire experiment time

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