Preparation method of microcapsule vaccines capable of resisting infection of aeromonas hydrophila

A vaccine preparation technology for Aeromonas hydrophila, which is applied in the field of vaccine preparation for the prevention and treatment of fish bacterial sepsis, and can solve the problems of time-consuming and labor-intensive, unfavorable large-scale promotion, etc.

Inactive Publication Date: 2011-08-03
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problem that immunotherapy by means of vaccine injection is time-consuming and laborious in the practice of fish farming, and is not cond

Method used

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  • Preparation method of microcapsule vaccines capable of resisting infection of aeromonas hydrophila
  • Preparation method of microcapsule vaccines capable of resisting infection of aeromonas hydrophila
  • Preparation method of microcapsule vaccines capable of resisting infection of aeromonas hydrophila

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Preparation of Aeromonas hydrophila suspension

[0029] Under sterile conditions, inoculate the pathogenic Aeromonas hydrophila strain S1 of Acipenser siberian bacterial septicemia stored on the slant medium of the test tube into the common nutrient broth with pH 7.2, shake at 30°C and 150 rpm Centrifuge at 3000 rpm for 20 min after shaking the bed for 48 h, wash with sterile saline for 3 times, and make the effective viable bacteria content of 10 10 siberian sturgeon bacterial septicemia pathogenic Aeromonas hydrophila strain S1 bacterial suspension, inactivated with formalin, and stored in a refrigerator at 4°C.

Embodiment 2

[0031] Preparation of microcapsule vaccine against Aeromonas hydrophila

[0032] Mix 200 ml of the inactivated Siberian sturgeon bacterial septicemia pathogenic Aeromonas hydrophila strain S1 strain S1 stored in the refrigerator at 4°C with 200 ml of 5% sodium alginate solution on a vortex shaker, turn on the blower, and press Then turn on the spray dryer, peristaltic pump and needle pump, and set the parameters as follows: air volume display 100%, inlet air temperature 115°C, outlet air temperature 48°C, feed rate 35 ml / h, needle pass frequency 5 s. Maintain an air flow of 600 m during spray drying 3 / h. After the gas passes through the blower, it is filtered and heated to a set temperature of 115°C. The peristaltic pump sends the material solution to the sprayer, and the microcapsule particles are collected by the cyclone separator and stored in a refrigerator at 4°C.

Embodiment 3

[0034] Determination of Immunogenicity of Aeromonas hydrophila Microcapsule Vaccine

[0035] 1. Randomly select 30 healthy 4-week-old female BABL / C mice, and randomly divide them into two groups, that is, the Siberian sturgeon bacterial sepsis pathogenic Aeromonas hydrophila strain S1 microcapsule vaccine mixed oral group and the control group , each set up 3 parallel. Among them, the BABL / C mice of the Siberian sturgeon bacterial septicemia pathogenic Aeromonas hydrophila strain S1 microcapsule vaccine mixed with the oral group were fed with Siberian sturgeon bacterial sepsis pathogenic Aeromonas hydrophila strain S1 every day. The feed of the microcapsule vaccine, the dosage of the Siberian sturgeon bacterial septicemia pathogenic Aeromonas hydrophila strain S1 microcapsule vaccine was 6 g / Kg feed, and the BABL / C mice in the control group were fed without Siberian sturgeon bacteria every day The additive dose of S1 microcapsule vaccine of pathogenic Aeromonas hydrophila str...

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Abstract

The invention discloses a preparation method of microcapsule vaccines capable of resisting the infection of aeromonas hydrophila, relating to the field of the preparation of antibacterial vaccines and solving the problems of time and labor wasting and difficult in large-scale popularization during the practical operations of cultivating fish and the like with immunotherapy by means of vaccine injection. The preparation method of the microcapsule vaccines capable of resisting the infection of the aeromonas hydrophila comprises the following steps of: (1) the preparation of aeromonas hydrophila suspension: inoculating the aeromonas hydrophila to prepare the aeromonas hydrophila suspension with the effective viable bacteria content of 1010 per ml, inactivating the aeromonas hydrophila suspension with formalin, and storing the suspension; and (2) the preparation of the microcapsule vaccines of the aeromonas hydrophila: uniformly mixing the aeromonas hydrophila suspension prepared in the step (1) and a 5% sodium alginate solution in the proportion of 1:1 in a vortex oscillator, and performing spraying and drying treatment on the mixture to obtain microcapsule particles. The method can be used for preparing the microcapsule vaccines for preventing and treating bacterial septicemia of sturgeon, particularly acipenser baeri.

Description

technical field [0001] The invention relates to a preparation method of a vaccine for preventing and treating fish bacterial septicemia, more particularly, relates to a preparation method of a microcapsule vaccine for preventing and treating Siberian sturgeon bacterial septicemia caused by Aeromonas hydrophila. Background technique [0002] Fish bacterial sepsis caused by Aeromonas hydrophila causes huge economic losses to fish farming every year. Current studies have shown that injection of an appropriate amount of inactivated Aeromonas hydrophila vaccine can significantly enhance the non-specific immune activity and anti-infection ability of fish. Mass promotion. [0003] The Siberian sturgeon belongs to the family Acipenseridae and the genus Acipenserus. It is one of the 26 existing species of sturgeon. It is mainly distributed in the Siberian rivers between the Ob River in the west of Russia and the Kolyma River in the east. Since my country started artificial breeding...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K9/50A61P31/04
Inventor 曹海鹏何珊吕利群杨先乐刘丽玲卫若鹏
Owner SHANGHAI OCEAN UNIV
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