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Grating filter membrane with sterilization indicating function

A grid and membrane technology, which is applied in membrane technology, semi-permeable membrane separation, sanitary equipment for toilets, etc., can solve the problems of grid filter confusion, affecting the results of microbial detection, and inability to identify filter membrane sterilization, etc. , to achieve the effect of convenient counting and avoiding confusing use

Inactive Publication Date: 2011-08-17
ZHEJIANG TAILIN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, no matter whether the laboratory adopts the above-mentioned sterilization measures for processing, or the aseptic packaging filter membrane provided by the manufacturer through industrial sterilization, there is this problem: the user is easy to mix the sterilized and non-sterilized cells. Grid filter membranes are confused with each other, thus affecting the results of microbial detection
But so far, all measures have not fundamentally solved this problem: it is impossible to identify whether the filter membrane itself has been sterilized, so as to confirm the sterility of the filter membrane

Method used

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  • Grating filter membrane with sterilization indicating function
  • Grating filter membrane with sterilization indicating function
  • Grating filter membrane with sterilization indicating function

Examples

Experimental program
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Effect test

Embodiment approach

[0023] Embodiments of the invention include:

Embodiment approach 1

[0025] Make a square dot-shaped dotted line screen printing template with a grid side length of about 3mm, inject high-pressure steam sterilization indicator ink (the ink can be purchased from the market, such as Guangzhou Yili Company) into the screen printing plate frame on the screen printing platform of the screen printing machine, Turn on the screen printing machine to print the ink on a white mixed cellulose ester flat film with a pore size of 0.45 microns, a width of about 400 mm, and a length of about 800 mm. After the ink is dry, remove the filter film and cut it into a diameter of about 50 mm. Pack the filter membrane with kraft paper and put it into a high-pressure steam sterilizer for sterilization. The sterilization condition is 121 degrees Celsius (0.1MPa), 15 minutes. Used for microbiological testing of purified water after sterilization.

Embodiment approach 2

[0027] Make a square dot-shaped dotted line screen printing template with a grid side length of about 3mm, inject ethylene oxide sterilization indicator ink (this ink can be purchased from the market) into the screen printing plate frame on the screen printing platform of the screen printing machine, and start the screen printing Machine-made ink is printed on a white nitrocellulose flat membrane with a pore size of 0.45 microns, a width of about 400mm, and a length of about 800mm. After the ink is dry, the filter membrane is removed and cut into a disc membrane with a diameter of about 50mm. , package the filter membrane with a dialysis paper bag, and put it into an ethylene oxide sterilizer for sterilization. Sterilization parameters: the temperature is 50°C, the relative humidity is 35%-70%, the gas concentration of ethylene oxide is 800mg / L, and the action time is 2 hours. It is used for microbial detection of drinking water after sterilization.

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Abstract

The invention discloses a grating filter membrane with a sterilization color-change indicating function, aiming at solving the problem that whether the filter membrane is sterilized can not be recognized and further the germ-free condition of the filter membrane can not be confirmed when microorganisms in liquid and air are separated by adopting a microporous membrane and being capable of conveniently counting colonies on the filter membrane. A grating line (2) on the filter membrane (1) in the invention is a water-based grating line. In order to realize the purpose of the invention, sterilizing color ink is printed on the surface of the filter membrane by adopting methods of silk-screen and the like, the ink and the filter membrane can have the same color and can have different colors, the grating line is changed from the original color into a sterilized color in color after being subjected to a sterilization mode suitable for indication ink and forms a remarkable color difference with the color of the filter membrane, therefore, the germ-free condition of the filter membrane can be confirmed and the microorganisms are conveniently counted. The invention can ensure the result of detecting the microorganisms, and can be widely applied to the detection of the microorganisms in liquid and air.

Description

technical field [0001] The invention mainly relates to a grid filter membrane, in particular to a grid filter membrane suitable for microorganism detection and having the function of sterilization and discoloration indication. Background technique [0002] The method of using microporous membranes to separate liquids and microorganisms in the air has long been widely used. For example, the conventional detection methods for determining the microbial content in water, beverages, beer, and pharmaceuticals are membrane filtration detection methods. The microorganisms in the liquid and air are retained on the surface of the filter membrane, and then the filter membrane is placed in a petri dish containing medium agar medium or other suitable medium for cultivation. Under certain temperature and time conditions, the bacteria on the filter membrane Microorganisms proliferate to form colonies visible to the naked eye. [0003] In order to facilitate the counting of the colonies fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/28B01D69/00
Inventor 赵振波夏信群邵晓云
Owner ZHEJIANG TAILIN BIOENG
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