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Human osteosarcoma cell strain and application thereof

A technology for human osteosarcoma and osteosarcoma, applied in tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of large differences in biological characteristics and low biodiversity of osteosarcoma, and achieve stable characteristics and high metastasis. potential effect

Inactive Publication Date: 2011-08-17
姚阳 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to provide a new human osteosarcoma cell line and its purpose

Method used

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  • Human osteosarcoma cell strain and application thereof
  • Human osteosarcoma cell strain and application thereof
  • Human osteosarcoma cell strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Establishment of primary osteosarcoma cell line SF-86 and identification of biological characteristics

[0063] 1. Experimental materials:

[0064] 1.1 Source of primary cell tissue and main reagents required for cell culture:

[0065] Tissue source: A 17-year-old male patient underwent high left thigh amputation in May 2008 due to osteosarcoma in the upper segment of the left femur. The pathological diagnosis of osteosarcoma (chondroblastoid type) was performed according to routine adjuvant chemotherapy for osteosarcoma. Adjuvant chemotherapy. In June 2008, lumps were found in the right upper arm and left buttock. In June 2008, tumors in the right upper arm and left buttock were resected. Postoperative pathology: metastatic osteosarcoma, immunohistochemistry: p53(+), Vim( +), Des(-), SMA(-), Ki67(+), CD117(-), CD99(+), Bcl-2(+), MG(-), in July 2008 multiple lungs were found metastases, and in August 2008, a metastases in the right subcutaneous soft tissue w...

Embodiment 2

[0152] Example 2 Establishment of Human Osteosarcoma High Metastasis Model Using Primary Osteosarcoma Cell Lines

[0153] 1. Experimental materials:

[0154] Twenty BALB / c-nu / nu nude mice were provided by the Experimental Animal Center of Shanghai Sixth People's Hospital, aged 3-4 weeks, male, weighing 15-18 g.

[0155] The experiments and feeding were carried out in the ultra-clean laminar flow frame under SPF conditions, and the sterilized water and SPF-grade special feed were freely ingested by the animals. The above experimental conditions were provided by the Experimental Animal Center of Shanghai Sixth People's Hospital.

[0156] For other experimental materials and reagents, please refer to the first part.

[0157] Antibodies used in immunohistochemistry of transplanted tumors were provided by the Department of Pathology, Shanghai Sixth People's Hospital.

[0158] 2. Experimental method:

[0159] 2.1 Cell culture conditions:

[0160] SF-86 cell line in conventional ...

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Abstract

The invention provides a human osteosarcoma cell strain and application thereof. The human osteosarcoma cell strain is collected in the China Center for Type Culture Collection with the collection number of CCTCC NO:C201002. The human osteosarcoma cell strain can be used for producing human osteosarcoma in a mammal to prepare a human osteosarcoma animal model, and can be further used for screening a drug candidate for treating human osteosarcoma. The human osteosarcoma cell strain provided by the invention has stable property, and can be stably subjected to passage repeatedly. The property of the human osteosarcoma cell strain keeps stable from the 30th generation to the 90th generation during in-vitro passage. The human osteosarcoma cell strain has the clinical biological property of human osteosarcoma, and particularly has the characteristics of primary drug resistance and high transfer potential. The human osteosarcoma cell strain plays an important role in solving the bottleneck problems of primary drug resistance and remote transfer which cannot be solved in the conventional human osteosarcoma treatment, and is an ideal cell strain for the basic research of human osteosarcoma and the preclinical phase application.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a human osteosarcoma cell line and its application. Background technique [0002] As early as 5,000 years ago on ancient Egyptian mummies, medical researchers discovered the existence of osteosarcoma (Marina N, et al. Biology and therapeutic advances for pediatric osteosarcoma. Oncologist 2004; 9(4): 422-41). Until now, osteosarcoma is the most common primary malignant bone tumor in children and adolescents. Especially before the age of 20, patients with osteosarcoma account for nearly 60% of all malignant bone tumor patients (Saeter G, Kloke O, JelicS, et al.ESMO Minimum Clinical Recommendations for diagnosis, treatment and follow-up ofosteosarcoma. Ann Oncol.2005 ; 16Suppl 1: i71-2), any age can be onset, but 75% of osteosarcoma patients are aged 10 to 25 years. Males are slightly more than females, the ratio is about 1.5:1. Typically, 80% to 90% of osteosarcomas occur in the me...

Claims

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Application Information

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IPC IPC(8): C12N5/09A01K67/027A61K49/00C12R1/91
Inventor 姚阳沈赞余文熙
Owner 姚阳
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