Swine influenza A H1N1 virus and use thereof

A type of influenza virus, influenza virus technology, applied in the direction of antiviral agents, viruses/phages, medical preparations containing active ingredients, etc., can solve the problem of lack of porcine H1N1 influenza virus serum antibodies and other issues

Inactive Publication Date: 2011-08-17
FUDAN UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

Pigs have always been considered as an important animal host for influenza virus gene recombination. Although both human and porcine H1N1 influenza viruses originated from poultry, the antigenicity of H1N1 influenza virus in pigs is relatively stable, and the human population lacks serum for porcine H1N1 influenza virus. Antibody

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  • Swine influenza A H1N1 virus and use thereof
  • Swine influenza A H1N1 virus and use thereof
  • Swine influenza A H1N1 virus and use thereof

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Embodiment Construction

[0044] The nasopharyngeal swab samples of 600 cases of healthy pigs slaughtered in slaughterhouses in Zhejiang, my country were collected for culture and identification. Seven strains of virus that can produce obvious cytopathic changes and can be stably propagated were isolated by cell culture, and were identified as swine influenza A H1N1 virus.

[0045] 1) Isolation and culture of pathogens: Throat swab specimens were processed and cultured according to the method recommended by WHO. Specimens were treated with penicillin and streptomycin (both at a final concentration of 100ug / ml) for more than 4 hours, centrifuged at 1500g at 4°C for 10 minutes, and the supernatant was inoculated into MDCK cells. Cultured under the condition of 5% CO2 at 37°C, observed the cytopathic effect (CPE) under an inverted microscope after 24 hours, and the pathological cells were stained with DIF and identified as influenza A virus by fluorescence microscope observation.

[0046] 2) Whole gene se...

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Abstract

The invention belongs to the field of microbial virology and provides a swine influenza A H1N1 virus. The virus contains eight fragments, namely HA, NA, NP, PB1, PB2, PA, NS and M, wherein the nucleotide sequences of the eight fragments are shown by the sequences from No.1 to No.8 in a nucleotide sequence table in the description in turn. In the invention, by separate culture of a nasopharyngeal swab sample of a healthy pig, differential item functioning (DIF) and reverse transcription-polymerase chain reaction (RT-PCR) identification and the comparison of full sequences of 8 genes of the virus, a virus is obtained and verified to be a swine influenza A H1N1 virus strain. The swine influenza A H1N1 virus strain is named A/swine/Zhejiang/26/2009(H1N1), with a collection number of CCTCC No.V201016. The strain obtained by the invention supplies valuable genetic information of swine influenza A virus in China, has a great significance for completing a system for monitoring the infection caused by the pathogen and can be used for preparing a reagent for quickly diagnosing infection with the influenza A H1N1 virus.

Description

technical field [0001] The invention belongs to the field of microbial viruses and relates to influenza virus strains, in particular to a novel strain of porcine type A H1N1 virus and its application. Background technique [0002] Influenza viruses are divided into three types: A, B, and C. Influenza A viruses are most likely to mutate. Influenza pandemics are caused by the emergence of new subtypes or the reappearance of old subtypes of influenza A viruses. The surface antigen of influenza A virus often undergoes small mutations. This mutation is called "drift". Figuratively speaking, "drift" means that the virus camouflages itself through small changes to avoid the human immune system. purpose of identification. As a result of the "drift" of influenza A virus, the strains that cause influenza may be different every year, and people need to re-vaccinate against influenza every year for prevention. "Shift" (shift) is mainly the result of the recombination of gene segments ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/145C12R1/93G01N33/569A61P31/16C12N7/00
Inventor 居丽雯蒋露芳姜庆五
Owner FUDAN UNIV
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