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Method for extracting cholesterol from animal brain dry powder with ultrasonic waves

An ultrasonic and cholesterol technology, applied in steroids, organic chemistry and other directions, can solve the problems of long immersion time, waste of animal brain resources, low yield of extracted cholesterol, etc., and achieve the effect of shortening the immersion time.

Inactive Publication Date: 2011-08-31
ANHUI KEBAO BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional technology uses acetone extraction method to extract cholesterol from animal brainstem powder. In this method, 10 times the amount of acetone solution is added to animal brainstem powder. The impregnation takes a long time (up to 72 hours), and the extraction cholesterol yield is low (<6%) , low purity (≤90%), and residual toxic organic solvents, the extracted substances can only be used as feed additives, resulting in a waste of animal brain resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] In the small laboratory test or sampling experiment, take 0.1kg of animal brain dry powder and put it into the experimental vessel, add 3 times the amount of acetone solution, let the animal brain dry powder soak in the acetone solution for 7 hours, so that it can be fully immersed;

[0010] Put the mixture of impregnated animal brain dry powder and acetone solution into an ultrasonic extraction kettle, set the ultrasonic emission frequency of the ultrasonic extraction kettle to 30-40KHz, the temperature to 25-28°C, and extract for 5-10 minutes to ensure the maximum Cholesterol is extracted to a certain extent, and filtered with a special plate frame to obtain the extract for subsequent use;

[0011] Put the extract into a rotary evaporator, set the vacuum degree of the rotary evaporator to 0.06Mpa, the temperature to 39-40°C, cool the circulating water to recover acetone, and obtain a yellow paste;

[0012] Take out the yellow paste, add 4 times the concentration of 96...

Embodiment 2

[0017] In the production process, take 10 kg of animal brain dry powder and put it into the experimental vessel, add 5 times the amount of acetone solution at this time, let the animal brain dry powder be immersed in the acetone solution for 9 hours, so that it is fully immersed;

[0018] Put the experimental vessel containing the solution into the ultrasonic extraction kettle, set the ultrasonic emission frequency of the ultrasonic extraction kettle to 40-50KHz, and the temperature to 30-35°C, extract for 10-15 minutes, and then filter with a special plate frame to obtain the extract , this process effectively ensures the yield of cholesterol;

[0019] Put the extract into the spherical concentrator, set the vacuum degree of the spherical concentrator to 0.08Mpa, set the temperature to 35-37°C, cool the circulating water to recover acetone, and obtain a yellow paste;

[0020] Put the yellow paste into a container, add 6 times the concentration of 93-95% ethanol solution into ...

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PUM

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Abstract

The invention relates to a method for extracting cholesterol from animal brain dry powder with ultrasonic waves. Animal brain dry powder is used as raw materials, and the method comprises the steps of: I, dipping before extraction; II, ultrasonic extraction; III, concentration and recovery of acetone; IV, hydrolysis, cooling and crystallization; V, dissolution and crystallization; VI, recrystallization; and VII making of finished products. The method has the advantages that first, the used quantity of a dipping solution for the animal brain dry powder is reduced (5 times of quantity is used to the largest); second, the dipping time is enormously shortened (the dipping lasts for 9h furthest); third, high-purity cholesterol (with purity of 95%-97%) can be obtained by three times of crystallization on the premise of guaranteeing high yield (up to 8.5%-10%), and multiple reflux and heating ensure no toxic organic solvent left.

Description

technical field [0001] The invention relates to an extraction method of effective components in animal brains, in particular to a method for extracting cholesterol in animal brain stem powder by ultrasonic waves. Background technique [0002] Animal (pig, cattle, sheep, etc.) brain stem powder contains a variety of rich substances, the main ingredients of which are important raw materials for medicine, cosmetics, liquid crystal materials, and aquatic feed additives. How to fully extract the active ingredients, become the key to whether it can be used. The traditional technology uses acetone extraction method to extract cholesterol from animal brainstem powder. In this method, 10 times the amount of acetone solution is added to animal brainstem powder. The impregnation takes a long time (up to 72 hours), and the extraction cholesterol yield is low (<6%) , low purity (≤90%), and residual toxic organic solvents, the extracted substances can only be used as feed additives, r...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 赵厚发张维秀徐宾朋郭庆
Owner ANHUI KEBAO BIOLOGICAL ENG CO LTD
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