Simple method for quickly collecting large quantity of female root-knot nematodes

Abstract

The invention discloses a simple method for quickly collecting a large quantity of female root-knot nematodes. The method comprises the following steps of: decomposing epithelial tissues and cortical tissues of the roots of decayed plants by using bacteria at the temperature of 25-30 DEG C; removing epidermis and cortexes of the roots of decomposed decayed plants, releasing internal females, sieving with a 20-mesh sieve and a 80-mesh sieve and collecting residues on the 80-mesh sieve; and directly picking white female root-knot nematodes with a thin writing brush under a stereoscopic anatomical lens or at a light-sufficient place. By adopting the method, a large quantity of female root-knot nematodes can be collected within a short period of time, no special instrument equipment is needed, and the defects such as time wasting, labor wasting and easily breaking of nematode bodies in the conventional technology are overcome.

Description

technical field

[0001] The invention relates to a simple method for obtaining a large amount of female root-knot nematodes. It is suitable for the research of phytopathology and phytonematology, the experimental teaching courses of phytopathology and phytonematology, and has extremely important application value in the taxonomy of root-knot nematodes. Background technique

[0002] The perineal pattern is an important morphological basis for the identification of root-knot nematode species. A large number of females are needed to prepare the slides of the perineal pattern and experimental teaching, and the female root-knot nematodes are all buried in the root tissue of the host. To obtain the females must be The root knot is dissected under a dissecting microscope, and the internal females are taken out. During the operation, the internal females cannot be seen clearly. Therefore, the females are often broken during dissection, so that it takes a lot of time to obtain a small...

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