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Achromobacter xylosoxidans MG1 strain and application thereof

A colorless bacillus and oxidized wood technology, applied in the direction of bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of less than 30%, and the treatment pass rate is only 57.6%, so as to reduce the treatment cost and expand the The effect of strain range

Inactive Publication Date: 2013-04-03
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Its wastewater treatment rate is less than 30%, and the qualified rate of treatment is only 57.6%.
There is no report about its decolorization activity and related mechanism

Method used

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  • Achromobacter xylosoxidans MG1 strain and application thereof
  • Achromobacter xylosoxidans MG1 strain and application thereof
  • Achromobacter xylosoxidans MG1 strain and application thereof

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Embodiment Construction

[0014] The following are embodiments of the present invention, but the content of the present invention is not limited thereto.

[0015] The present invention is realized as follows:

[0016] After screening and purifying Achromobacter xylosoxidans, it was domesticated with malachite green several times. Then test its final decolorizing activity. And explore the decolorization mechanism inside and outside the cell.

[0017] Methods of purification, culture and domestication of Achromobacter xylosoxidans MG1 (the following are weight percentages):

[0018] 1. Plate purification culture

[0019] The LB plate medium formula is: 0.5% YeastExtract, 1% Tryptone, 1% NaCl, 1.5-2% Agar, natural pH. The mixed bacteria were inoculated on a plate medium and cultured at 37°C to obtain a single colony.

[0020] 2. Liquid expansion culture

[0021] The formula of LB liquid medium is: 0.5% Yeast Extract, 1% Tryptone, 1% NaCl, the rest is water, and the pH is natural. Inoculate a single colony on the ...

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Abstract

The invention relates to an achromobacter xylosoxidans MG1 strain and application thereof, and belongs to the field of environmental application of microorganisms. The achromobacter xylosoxidans MG1 strain is prepared by screening bacteria obtained by separating printing and dyeing wastewater by the conventional method, and was collected in China General Microbiological Culture Collection Center on December 15, 2010; and the collection number is CGMCC NO.4476. The achromobacter xylosoxidans MG1 strain has ultrastrong efficiency of decoloration on malachite green in triphenylmethane dyes, and the decolorization rate at 1 hour is up to 86+ / -1 percent when the concentration of the malachite green is up to 2,000mg / L. In addition, the MG1 strain also has a better effect of decolorizing crystalviolet, and can be applied to the preparation of biological decoloring agents. The achromobacter xylosoxidans MG1 strain has the advantages that: the range of strains which can degrade dyes can be broadened, so that an additional artificial degradation way is provided for the malachite green and the crystal violet in the environment, and the treatment cost for environmental pollution is reduced.

Description

Technical field: [0001] The invention relates to a strain of Achromobacter xylosoxidans MG1 and its application, and belongs to the field of microbial environmental applications. Background technique: [0002] With the development of science and technology, synthetic dyes are widely used in textile, leather, food, daily chemical industries and other industries. However, these dyes usually contain complex aromatic ring structures, high chemical stability, low environmental degradability, and most dyes and their metabolic intermediates have mutagenicity, carcinogenicity and other toxicities, which also makes dyes an important environmental pollution One of the things. As a source of pollution, the first characteristic of dyes is the large amount of pollution. According to statistics, the current world's annual output of dyes is about 800,000 to 900,000 tons, and my country's annual output of dyes reaches 150,000 tons, ranking among the top in the world. About 10% to 15% of the d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/025
Inventor 黄晓玮乔敏王纪爱周薇张克勤
Owner YUNNAN UNIV
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