Auxiliary screening method for stripe rust-resistance wheat and its special primers

A stripe rust, wheat technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of accelerated directional selection of pathogenic strains, loss of disease resistance, etc.

Inactive Publication Date: 2011-10-19
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the high resistance of the physiologically specialized disease resistance gene to stripe rust, it is very popular among breeders. However, when a variety with a single disease resistance gene is planted in a large area, the directional selection of the pathogen race will be accelerated and the growth of the dominant race population will be nurtured. , causing rust-resistant varieties to lose their disease resistance after a few years of large-scale application in production

Method used

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  • Auxiliary screening method for stripe rust-resistance wheat and its special primers
  • Auxiliary screening method for stripe rust-resistance wheat and its special primers
  • Auxiliary screening method for stripe rust-resistance wheat and its special primers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1, the acquisition of QYr.caas-2BL marker Xgwm501195

[0043] 1. Discovery of the QYr.caas-2BL gene and linkage markers

[0044] Zhou 8425B showed high resistance to Tiaozhong 32 at the adult stage. The main parent of winter wheat, 8425B, and its 80 derived varieties (lines) were selected, including 8 copies of the first generation, 35 copies of the second generation, and 37 copies of the third generation.

[0045] 1. Field design and investigation

[0046] They were planted in Anyang and Zhoukou, Henan respectively in 2009-10, using completely randomized block design, with 3 repetitions, 6 rows per plot, row spacing 20cm, and row length 4m. One row of high-susceptibility wheat stripe rust variety Mingxian 169 was planted in every two plots as an inducer. Field identification of disease resistance uses a mixed race dominated by the dominant physiological race CYR32 of stripe rust.

[0047] The field inoculation was carried out at the 3-4 leaf stage of wheat....

Embodiment 2

[0061] Embodiment 2, Xgwm501 195 Flagged apps

[0062] Zhoumai 11, Zhoumai 12, Zhoumai 13, Zhoumai 16, Zhoumai 17, Zhoumai 22, 04zhong 36, Luomai 22, Bainong AK58, Fu 98-46 are descendants of Zhou 8425B. Zhengmai 9023, Yumai 34, Yumai 70, Zhongyou 16, Zhongyou 9507, Zhongyou 9701, Xiaoyan 54, Yuandong 6, Beijing 10 and Jing 411 are not descendants of Zhou 8425B.

[0063] 1. Detection of stripe rust resistance of different varieties of wheat

[0064] The stripe rust resistance identification of wheat varieties was carried out in the greenhouse of the Institute of Crop Science, Chinese Academy of Agricultural Sciences in the winter of 2010. Wheat stripe rust race (Puccinaia striiformis f.sp. tritici, Wan Anmin, Wu Liren, Jin Shelin, Yao Ge, Wang Baotong. Nomenclature and characteristics of wheat stripe rust fungus Tiaozhong 32 in China. Plant Protection Acta Sinica, 2003, 30(4): 347-352., publicly available from the Institute of Crop Science, Chinese Academy of Agricultural S...

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Abstract

The invention discloses an auxiliary screening method for rust-disease-resistance wheat and its special primers. The invention provides a PCR reagent for the auxiliary screening of rust-disease-resistance plants, comprising a primer pair composed of primers with nucleotide sequences of SEQ ID No:1 and SEQ ID No:2 in the sequence table and a PCR amplification buffer. The invention provides a novelmajor effect gene site QYr.caas-2BL and SSR primer Xgwm501 of the auxiliary screened rust-disease-resistance wheat of the site, the mark can accurately identify the rust-disease-resistance gene QYr.caas-2BL. The special primers and molecular markers provided by the invention can play an important role in breeding of wheat for disease resistance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for assisting in screening wheat resistant to stripe rust and special primers thereof. Background technique [0002] Stripe rust caused by wheat stripe rust (Puccinia striiformis f.sp.tritici) is a widely distributed airborne disease that occurs in almost all major wheat producing countries, especially in cold and high altitude regions. my country is the largest endemic area of ​​wheat stripe rust in the world, and wheat stripe rust mainly occurs in the northwest and southwest regions of China. From the 1950s to the present, there have been 15 moderate epidemics and 7 large-scale wheat stripe rust epidemics in my country. The area of ​​the first occurrence of wheat stripe rust reached more than 200 million mu, and the area of ​​the last two outbreaks was more than 110 million mu, with a loss of 600, 3 million, 2.65 and 1 million tons of wheat respectively (Wan Anmin et al., ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 何中虎肖永贵夏先春
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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