Hepatocyte large-scale culturing and freezing device

Abstract

The invention provides a hepatocyte large-scale culturing and freezing device. The device comprises a culture box and a freeze box, wherein the culture box comprises a box body, at least two layers of flat plates, a chitosan nano-fiber film, flat plate pores, an upper plug cover, a support pipe, a support side hole and a lower plug cover; the freeze box comprises a freeze box body, a refrigerant and a freeze box cover; and the culture box is placed in a refrigerating fluid in the freeze box. The hepatocyte prepared through primary separation can be directly inoculated onto the multi-layer flat plates of chitosan in the culture box according to requirements to perform amplification, so that large-scale culture is realized; the chitosan nano-fibers on the surfaces of the multi-layer flat plates in the culture box can sufficiently simulate an in-vivo microenvironment to promote adhesion of the hepatocyte and improve the cell activity and functions of defreezed hepatocyte. The hepatocyte large-scale culturing and freezing device has the advantages of being reasonable in structure design, simple, convenient and practical in operation and use and the like, and is suitable for being used in hospitals requiring massive hepatocytes for patients with liver failure.

Description

technical field [0001] The invention relates to a device, especially a device mainly used for large-scale culture and freezing of hepatocytes, which belongs to a container specially used for medical purposes. Background technique [0002] Patients with chronic hepatitis cirrhosis and advanced liver cancer often die of end-stage liver failure. The application of bioartificial liver and liver transplantation to treat liver failure has made remarkable achievements in recent years. The bioartificial liver support system removes various harmful substances in the body and supplements the necessary proteins and coagulation factors with the help of an extracorporeal circulation device to provide short-term liver function support for patients; liver transplantation therapy uses allogeneic or xenogeneic liver transplantation into the patient's body to play its role. function to achieve therapeutic purposes. However, both of the above two treatment methods are facing the problem of la...

AI Technical Summary

Problems solved by technology

First of all, the amount of hepatocytes required for treatment is large, and the conventional cryotube freezing method has a small number and cumbersome operation process, which cannot meet the requirements of large-scale freezing; secondly, the conventional freezing method cannot meet the urgent need for a large number of hepatocytes at one time. , let alone achieve the goal of finished product output

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