Dual PCR (Polymerase Chain Reaction) quick detection method for rabbit hemorrhagic disease virus and rabbit pasteurellosis
A technology of rabbit haemorrhagic disease virus and rabbit Pasteurella, which is applied in the field of detection of rabbit virus and pathogens, can solve the problems of undiscovered rabbit haemorrhagic disease virus, double PCR rapid detection method of rabbit Pasteurella, etc.
Active Publication Date: 2013-03-13
金湖县农副产品营销协会
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[0004] After searching, no relevant research reports on double PCR rapid detection method for rabbit haemorrhagic disease virus and rabbit pasteurella were found
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The invention discloses a dual PCR (Polymerase Chain Reaction) quick detection method for a rabbit hemorrhagic disease virus and rabbit pasteurellosis. In the method, two pairs of specific primers capable of amplifying a VP60 gene and a kmt gene are designed according to a rabbit hemorrhagic disease virus VP60 gene and a rabbit pasteurellosis kmt gene sequence. A specific stripe of 645 bp occurring in an electrophoretogram indicates a positive rabbit hemorrhagic disease virus; and a specific stripe of 215 bp occurring in the electrophoretogram indicates positive rabbit pasteurellosis. As proved by a research, the method can be used for detecting the rabbit hemorrhagic disease virus and the rabbit pasteurellosis in the same reaction system, has the characteristics of quickness, specificity, sensitivity and stability, can be applied to quick differential diagnosis of a rabbit hemorrhagic disease and a rabbit pasteurellosis disease, and plays an important role in effectively preventing and treating the two diseases; and the defects of the conventional diagnostic method are overcome.
Description
technical field [0001] The invention relates to the detection technology of rabbit virus and germ, in particular to a double PCR rapid detection method of rabbit haemorrhagic disease virus and rabbit Pasteurella. Background technique [0002] Rabbit hemorrhagic disease (commonly known as rabbit plague, Rabbit hemorrhagic disease, referred to as RHD) is a severe infectious disease caused by rabbit hemorrhagic disease virus (RHDV), which is highly contagious, has a short course of disease, and has a high incidence rate. And mortality rate is all very high, and in the rabbit field of acute outbreak, morbidity and mortality rate can reach 100%. Rabbit pasteurellosis is an acute infectious disease caused by Pasteurella multocida, which often causes a large number of rabbits to become ill and die. These two diseases have brought huge economic losses to the rabbit industry and seriously restricted the development of the rabbit industry. Because the clinical symptoms and pathologi...
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IPC IPC(8): C12Q1/70C12Q1/68C12Q1/04C12R1/93C12R1/01
Inventor 吴健敏黄红梅刘梅覃绍敏白安斌
Owner 金湖县农副产品营销协会
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