Detection method for antisperm antibody in cervical mucus of dairy cows

Abstract

The invention discloses a detection method for an antisperm antibody in the cervical mucus of dairy cows, comprising the steps of: 1) taking cervical mucus of the dairy cow in estrus; 2) taking bull fistula frozen sperm, centrifuging and removing supernatant, adding EBSS (Earle's Balanced Salt Solution) suspension, laying the suspension at the bottom of a test tube in which the EBSS containing 10% fetal calf serum is pre-arranged, incubating for 45 min in an incubator, taking the supernatant; 3) adding pineapple protease liquid in the cervical mucus defrosted at room temperature and centrifuging; and 4) diluting the supernatant in the step 3) by using EBSS, adding 15 microliters of supernatant prepared in the step 2) and mixing, covering 20 microliters of paraffin oil, incubating for 30 min, wherein the number of the agglutinated sperm is recorded as N, the total number of sperms is recorded as Nz, the antisperm antibody is judged to be positive when N / Nz is more than and equal to 10%, and the antisperm antibody is judged to be negative when N / Nz is less than 10%. According to the detection method, the antisperm immune infertility cows in the dairy cows can be easily and rapidly detected.

Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a method for detecting antisperm antibodies in cervical mucus of dairy cows. Background technique

[0002] Antisperm antibody (antisperm antibody, AsAb) can combine with sperm to produce immune reaction and affect reproductive ability. Sperm are antigenic to males and sperm are alloimmune antigens to females, and each mating is equivalent to an immunization. Local and serum antisperm antibodies in the reproductive tract are the product of the body's own or allogeneic immune response to sperm antigens, and are the main cause of immune infertility. The core of dairy cow production is reproduction, and preventing infertility is the primary problem to be solved. The impact of antisperm antibodies on infertility is common in dairy cows, and a considerable part of antisperm immune infertile cows can be prevented by appropriate targeted technical measures. Imm...