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Bcl11a siRNA-585 for inhibiting B-cell lymphoma/leukemia 11A (BCL11A) expression and tumorous B cell proliferation and application thereof

A technology of bcl-2siRNA and B cells, applied in the direction of DNA / RNA fragments, anti-tumor drugs, recombinant DNA technology, etc., can solve the problems of lack of Bcl11asiRNA, etc., and achieve the effect of huge market potential

Inactive Publication Date: 2012-01-25
JINAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0004] Existing studies have shown that BCL11A may be associated with the occurrence of B-cell tumors, but there is no evidence that Bcl11a siRNA can be used in tumors, especially in the treatment and / or prevention of B-cell tumors

Method used

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  • Bcl11a siRNA-585 for inhibiting B-cell lymphoma/leukemia 11A (BCL11A) expression and tumorous B cell proliferation and application thereof
  • Bcl11a siRNA-585 for inhibiting B-cell lymphoma/leukemia 11A (BCL11A) expression and tumorous B cell proliferation and application thereof
  • Bcl11a siRNA-585 for inhibiting B-cell lymphoma/leukemia 11A (BCL11A) expression and tumorous B cell proliferation and application thereof

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Embodiment 1

[0035] 1. Experimental materials and methods

[0036] (1) Design and synthesis of Bcl11a siRNA

[0037] According to the design tool software provided by Ambion on the Internet (see www.ambion.com / techlib / misc / siRNA finder.html), three siRNA sequences were designed for the Bcl11a gene, named Bcl11a siRNA-585, Bcl11a siRNA-475 and Bcl11a respectively siRNA-319, negative siRNA labeled with green fluorescent FAM at the 5' end of the positive sense strand and siRNA with irrelevant sequence were designed at the same time as a control, which was synthesized by Shanghai Jikai Gene Chemical Technology Co., Ltd.

[0038]The sequence of Bcl11a siRNA-585 is as follows:

[0039] Sense strand: 5'-GAAUCUACUUAGAAAGCGAUU-3';

[0040] Antisense strand: 5'-UCGCUUUCUAAGUAGAUUCUU-3';

[0041] The sequence of Bcl11a siRNA-475 is as follows:

[0042] Sense strand: 5'-GCAGGGUAUUUGUAAAGAUUU-3';

[0043] Antisense strand: 5'-AUCUUUACAAAUACCCUGCGG-3';

[0044] The sequence of Bcl11a siRNA-319 is ...

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Abstract

The invention discloses Bcl11a siRNA-585 for inhibiting B-cell lymphoma / leukemia 11A (BCL11A) expression and tumorous B cell proliferation. The sequence of the sense strand of the Bcl11a siRNA-585 is 5'-GAAUCUACUUAGAAAGCGATT-3'; and the sequence of the antisense strand of the Bcl11a siRNA-585 is 5'-UCGCUUUCUAAGUAGAUUCTT-3'. The Bcl11a siRNA-585 can efficiently inhibit BCL11A expression, effectively inhibit tumorous B cell proliferation and promote tumorous B cell apoptosis. The invention also proves that the tumorous B cell proliferation is inhibited more effectively and the tumorous B cell apoptosis is promoted by combining the Bcl11a siRNA-585 and Bc1-2 siRNA. The Bcl11a siRNA-585 for inhibiting the BCL11A expression and the tumorous B cell proliferation has significance for developing new anti-B cell tumor gene medicaments and improving the treatment effect of B cell tumors.

Description

technical field [0001] The invention relates to the field of siRNA and its application, in particular to a Bcl11a siRNA-585 inhibiting the expression of BCL11A and the proliferation of tumorous B cells and its application in the preparation of drugs for treating and / or preventing B cell tumors. Background technique [0002] RNAi technology is a new way to inhibit gene expression with high efficiency and high specificity. In vivo and in vitro studies have shown that RNAi technology has better application prospects than antisense nucleic acid in cancer gene therapy. A large number of studies have shown that inhibiting the expression of tumor-related genes by RNAi in vitro can inhibit the proliferation and growth of tumor cells, induce apoptosis, and increase the sensitivity of tumor cells to drugs. [0003] The Bcl11a (B-cell lymphoma / leukemia 11A) gene is located at 2p16.1 and is a krüppel-like zinc finger gene belonging to the BCL11 family (including BCL11A and BCL11B). Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P35/00A61K48/00C12N15/113A61P35/02
Inventor 何冬梅高杨军李扬秋
Owner JINAN UNIVERSITY
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