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Application of Astragaloside IV

A technology of astragaloside IV and medicine, which is applied in the field of application of astragaloside IV in the preparation of drugs for the prevention and treatment of Alzheimer's disease, and can solve the unseen research reports on the preparation of drugs for the prevention and treatment of Alzheimer's disease by astragaloside IV And other issues

Inactive Publication Date: 2012-03-28
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no relevant research reports on the preparation of astragaloside IV in the preparation of drugs for the prevention and treatment of Alzheimer's disease.

Method used

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  • Application of Astragaloside IV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Effect of astragaloside IV on secretion of Aβ protein by SH-SY5Y-APP695sw transgenic cells

[0032] Test materials: SH-SY5Y-APP695sw transgenic cells were preserved by the Natural Medicine Engineering Research Center of the Ministry of Education of Beijing Normal University; astragaloside IV was purchased from the China Institute for the Control of Pharmaceutical and Biological Products, and the HPLC detection content was >98%; G418 was purchased from Ameresco; cell culture Reagents, Aβ40 Colorimetric ELISA Kit and Aβ42 Colorimetric ELISA Kit were purchased from Invitrogen.

[0033] Cell culture: SH-SY5Y-APP695sw transgenic cells were incubated with DMEM / F12 complete medium containing 10% fetal bovine serum, 100 U / mL penicillin, and 100 mg / L streptomycin at 37°C, 5% CO 2 , cultured in an incubator with a relative humidity of 90%. An additional 500 μg / mL G418 was added to the medium to maintain the expression of exogenous genes.

[0034] Test method: SH-SY5Y...

Embodiment 2

[0036] Example 2 The effect of astragaloside IV on the shearing action of APP protein

[0037] Test materials: SH-SY5Y-APP695sw and SH-SY5Y-C99 transgenic cells were preserved by the Natural Medicine Engineering Research Center of the Ministry of Education of Beijing Normal University; From Ameresco Company, cell culture reagents were purchased from Invitrogen Company; BCA protein concentration assay kit was purchased from Saichi Biotechnology Company; anti-sAPPα monoclonal antibody 2B3, anti-sAPPβsw monoclonal antibody 6A1 and anti-APP polyclonal antibody were purchased from IBL Company; Anti-total sAPP monoclonal antibody 22C11 was purchased from Millipore Company; anti-β-actin monoclonal antibody C4 was purchased from Santa Cruz Company; Amersham ECL plus chromogenic reagent was purchased from GE Company.

[0038] Cell culture: see Example 1.

[0039] Test method: SH-SY5Y-APP695sw and SH-SY5Y-C99 transgenic cells were divided into 2×10 5 Cells / well were seeded in 6-well p...

Embodiment 3

[0041] Example 3. Effect of Astragaloside IV on β-secretase (BACE1) protein expression and enzyme activity in SH-SY5Y-APP695sw transgenic cells

[0042] Test materials: SH-SY5Y-APP695sw transgenic cells were preserved by the Natural Medicine Engineering Research Center of the Ministry of Education of Beijing Normal University; astragaloside IV was purchased from the China Institute for the Control of Pharmaceutical and Biological Products, and the HPLC detection content was >98%; the anti-BACE1 polyclonal antibody was purchased from Merck Company and anti-β-actin monoclonal antibody C4 were purchased from Santa Cruz Company; Amersham ECL plus chromogenic reagent was purchased from GE Company; β-secretase activity assay kit was purchased from Biovision Company; microplate reader (Thermo Labsystems Multiskan MK3 type ).

[0043] experiment method:

[0044] β-secretase (BACE1) protein expression assay: SH-SY5Y-APP695sw cells were mixed with 2×10 5 Cells / well were seeded in a 6-...

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PUM

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Abstract

The invention relates to the field of traditional Chinese medicines, and discloses application of Astragaloside IV in the preparation of medicaments for preventing and treating Alzheimer disease. Tests show that the Astragaloside IV can inhibit the secretion of Abeta proteins and beta-secretases, the enzyme activity of the beta-secretases and the enzyme cutting effect of the beta-secretases in SH-SY5Y-APP695sw cells, thereby inhibiting the accumulation of the Abeta proteins. Meanwhile, tests show that the Astragaloside IV can promote oligomerized Abeta degradation, thereby reducing the toxic effect of the Abeta proteins and achieving the purpose of preventing and treating Alzheimer disease. Thus, the invention provides the application of the Astragaloside IV in the preparation of medicaments for preventing and treating Alzheimer disease.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to the application of astragaloside IV in the preparation of drugs for preventing and treating Alzheimer's disease. Background technique [0002] Astragalus is a commonly used traditional Chinese medicine. It is sweet in taste and warm in nature. Studies have found that compounds such as astragalus polysaccharides, saponins, and flavonoids contained in Astragalus have strong biological activities. [0003] Astragaloside IV (Astrogaloside-IV, AGS-IV) is a triterpenoid saponin compound extracted from the traditional Chinese medicine Astragalus membranaceus, and its molecular formula is C 41 h 68 o 14 , the molecular weight is 784.97, and the structural formula is as follows: [0004] [0005] Modern pharmacological studies have shown that astragaloside IV has anti-inflammatory, anti-aging, anti-virus, scavenging free radicals, promoting insulin secretion, and enhanci...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P25/28
Inventor 张文生马涛
Owner BEIJING NORMAL UNIVERSITY
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