HSCGF liposome as well as preparation and application thereof

A growth factor and stem cell technology, applied in the field of human stem cell growth factor liposome and its preparation, can solve the problems of easy inactivation, short half-life, low bioavailability, etc., to improve bioavailability, wrinkle repair and wrinkle removal , promote the effect of absorption and utilization

Active Publication Date: 2014-08-27
GUANGZHOU SALIAI BIOLOGICAL GENETIC ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As a biologically active protein, SCGF has the characteristics of poor stability at room temperature, easy degradation, and easy inactivation; low bioavailability, short half-life, and rapid metabolism. Disadvantages such as low bioavailability

Method used

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  • HSCGF liposome as well as preparation and application thereof
  • HSCGF liposome as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Weigh 1g of dry soybean lecithin, 0.1g of vitamin E and 0.35g of cholesterol into an eggplant-shaped flask, dissolve it with 5-20ml of absolute ethanol, place the eggplant-shaped flask in a constant temperature water bath at 50°C to evaporate under reduced pressure, and pump to absolute ethanol Shake up to form a uniform film, add 10ml of phosphate buffer solution (pH6.8, 10mmol / L) containing 400mg SCGF (hSCGF-α, purchased from Peprotech company), mass volume ratio 24% sucrose, and spin to wash the membrane, and finally shake Hydrate the solution for 30-60 minutes to obtain SCGF phospholipid suspension (human stem cell growth factor liposome).

Embodiment 2

[0038] Weigh 0.9g of dry soybean lecithin, 0.1g of vitamin E and 0.3g of cholesterol into an eggplant-shaped flask, dissolve them with 5-20ml of absolute ethanol, put the eggplant-shaped flask in a constant temperature water bath at 50°C and vacuum rotary evaporation, and pump until dry Ethanol was evaporated to form a uniform film, and 10ml of phosphate buffer solution (pH6.8, 10mmol / L) containing 300mg SCGF (hSCGF-α, purchased from Peprotech Company) and 10% sucrose by mass to volume ratio was added to wash the film, and the film was hydrated. The solution is passed through a high-pressure homogenizer (100 MPa, 5 times) to obtain a translucent liposome colloid solution (human stem cell growth factor liposome) with a particle size in the range of 100 to 200.

Embodiment 3

[0040]Precisely weigh 800mg of phospholipids and 250mg of cholesterol, place them in a 25ml eggplant-shaped bottle, add chloroform to dissolve them and make the volume to 10ml, inject nitrogen gas, and ultrasonicate at 100W for 10min on an ice bath, once every 30s, for a total of 10 times. Sonicated liposomes were prepared. Then add chloroform equivalent to 2 to 6 times the volume of the ultrasonic liposome to dissolve it, rotate it in a water bath at 35 to 45° C., remove all organic solvents, and then add 200 mg of SCGF (hSCGF-α, purchased from Peprotech Company) , 2.0g dextran and 1.5g sucrose in phosphate buffered saline solution (pH6.8, 10mmol / L), filled with nitrogen gas, hydrated in an ice bath for 12h, intermittently ultrasonicated for 10min (ultrasonic power 100W, ultrasonically once every 30s) . After ultrafiltration with a 0.22 μm microporous membrane, add mannitol with a mass volume ratio of 10%, vacuum freeze-dry, and immediately rehydrate the lipid with an approp...

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Abstract

The invention discloses an HSCGF (Human Stem Cell Growth Factor) liposome as well as the preparation and the application thereof. The HSCGF liposome comprises 2.0 to 4.0 percent of HSCGF, 8.0 to 10.0 percent of phosphatide, 2.5 to 3.5 percent of cholesterol and 10 to 25 percent of protecting agents. The HSCGF is prepared into liposome, the activity of the HSCGF is kept effectively, the stability of SCGF (Stem Cell Growth Factor) can be improved obviously, skin can be promoted to absorb and utilize the SCGF, and the HSCGF liposome has the advantages of good affinity, no toxicity or side effects, and wide application range. The anti-wrinkle performance of cosmetics containing the HSCGF coated with the liposome can still be kept well after the stability is examined for three months. The HSCGF liposome is used as raw material and other active materials and auxiliary materials are used as supplements so as to prepare frequently-used medicine forms to illustrate the drug effect of the SCGF, so that the bioavailability can be improved.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations and cosmetics, and in particular relates to a human stem cell growth factor liposome and a preparation method and application thereof. Background technique [0002] Human stem cell growth factor (hSCGF, also known as LSLCL) is an early hematopoietic stimulating factor that acts in the bone marrow hematopoietic microenvironment, has a variety of biological activities, and is mainly distributed in the brain, liver, bone marrow, Placenta, testis, kidney, etc. are produced by stromal cells, fibroblasts, endothelial cells, egg cells, podocytes, and liver cells in these tissues (especially bone marrow). Its gene is located on human chromosome 19q13.3 and consists of 4 exons and 3 introns. It is known that human stem cell growth factor has two forms, the full-length molecule hSCGF-α (the complete open reading frame of mature mRNA is 972bp, encoding 323 amino acids, and the expressed protein si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61K9/127A61K8/64A61P17/02A61P17/10A61Q1/04A61Q1/08A61Q1/12A61Q9/02
Inventor 王一飞陈海佳舒辉萍杨珂刘薇任哲
Owner GUANGZHOU SALIAI BIOLOGICAL GENETIC ENG CO LTD
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