Extraction method of nettle extract

A kind of nettle extract, extraction method technology, applied in the field of medicine, can solve the problem of low yield of nettle extract

Active Publication Date: 2012-06-27
JILIN HONGJIU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The yield of nettle extract obtained by the current extraction process is not high,

Method used

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  • Extraction method of nettle extract
  • Extraction method of nettle extract

Examples

Experimental program
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Embodiment 1

[0026] The preparation of embodiment 1 nettle extract

[0027] Take 100g of Urtica angustifolia, crush it, add 15 times the amount of water, extract at 15°C for 10 minutes at an ultra-high pressure of 300MPa, filter the extract, and concentrate to relative density at a temperature of 70°C and a vacuum of 0.06MPa 1.12 (70°C), let the concentrate cool down, put it into a 22mm semi-permeable membrane dialysis bag, place it in a stainless steel bucket filled with phosphate buffer, and dialyze it at 5°C to remove salt and some small molecular impurities , during which the buffer was changed 3-4 times. Take the retentate in the dialysis bag, use the sevage method to deproteinize, remove the white flocculent precipitate in the middle, and concentrate the water layer to a relative density of 1.12 (70°C) at a temperature of 70°C and a vacuum of 0.06MPa to obtain an infusion Paste 52g, add 1 times the amount of 95% ethanol for ethanol precipitation, let it stand for 20 hours, collect t...

Embodiment 2

[0032] The preparation of embodiment 2 nettle extracts

[0033] Take 100g of nettle medicinal material, add 10 times the amount of water after crushing, extract at 35°C for 8 minutes at an ultra-high pressure of 500MPa, filter the extract, and concentrate it to a relative density of 1.15 ( 80°C), let the concentrated solution cool, put it into a 22mm semi-permeable membrane dialysis bag, place it in a stainless steel bucket filled with phosphate buffer, and perform dialysis at 3°C ​​to remove salt and some small molecular impurities. Change the buffer 3-4 times. Take the retentate in the dialysis bag, use the sevage method to deproteinize, remove the white flocculent precipitate in the middle, and concentrate the water layer to a relative density of 1.15 (80°C) at a temperature of 80°C and a vacuum of 0.06MPa to obtain an infusion Paste 54g, add 3 times the amount of 80% ethanol for ethanol precipitation, let it stand for 24 hours, collect the sediment and centrifuge, recover...

Embodiment 3

[0038] The preparation of embodiment 3 nettle extracts

[0039] Take 100g of nettle medicinal material, add 13 times the amount of water after crushing, extract at 25°C for 9 minutes at an ultra-high pressure of 400MPa, filter the extract, and concentrate it to a relative density of 1.13( 70°C), let the concentrate cool down, put it into a 22mm semi-permeable membrane dialysis bag, place it in a stainless steel bucket filled with phosphate buffer, and perform dialysis at 4°C to remove salt and some small molecular impurities. Change the buffer 3-4 times. Take the retentate in the dialysis bag, use the sevage method to deproteinize, remove the white flocculent precipitate in the middle, and concentrate the water layer to a relative density of 1.13 (70°C) at a temperature of 73°C and a vacuum of 0.06MPa to obtain the leached Paste 50g, add 1.5 times the amount of 90% ethanol for ethanol precipitation, let it stand for 22 hours, collect the sediment and centrifuge, recover ethan...

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Abstract

The invention relates to the technical field of medicaments, in particular to an extraction method of a nettle extract. The yield of the nettle extract obtained by the extraction method is 46.5 to 49.6 percent. The nettle extract can be used for preparing the medicaments for treating prostatitis, prostatomegaly, benign hyperplasia of prostate or high blood pressure and can effectively treat prostatitis, prostatomegaly, benign hyperplasia of prostate or high blood pressure. The effective rate of the nettle extract on acute prostatitis is 93.33 percent; the effective rate of the nettle extract on chronic prostatitis is 90.33 percent; the effective rate of the nettle extract on prostatomegaly is 89.0 percent; the effective rate of the nettle extract on benign hyperplasia of prostate is 90.67 percent; and the effective rate of the nettle extract on high blood pressure is 91.0 percent.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for extracting nettle extract. Background technique [0002] Nettle is a perennial wild herb of Urticaceae Urtica. This plant is a traditional wild plant used for both medicine and food. It is rich in protein, polysaccharides, flavonoids, amino acids and other components. The plant can be used as wild vegetables when it is young, and has medicinal value after flowering. Nettle has the effect of expelling wind, relieving convulsions, eliminating food and defecating. It is used for rheumatic joint pain, postpartum convulsions, infantile convulsions, sequelae of polio, high blood pressure, indigestion, and constipation; external use for early urticaria, snake bites . Modern research has proved that nettle has a good curative effect on benign prostatic hyperplasia in men. In clinical treatment, nettle has long been used in the treatment of urticaria and rheumatic joint p...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P13/08A61P9/12
Inventor 冯涛金爱玉白凤英
Owner JILIN HONGJIU BIO TECH
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