Recombination blue-green alga for producing lactic acid as well as preparation method and applications thereof

Abstract

The invention discloses a recombination blue-green alga for producing a lactic acid as well as a preparation method and applications thereof. The method disclosed by the invention comprises the following steps of: modifying a metabolic pathway, and importing a coding gene of D-lactic dehydrogenase into a genome of the blue-green alga to obtain the recombination blue-green alga for producing an optical pure D-lactic acid. An amino acid sequence of the D-lactic dehydrogenase is a sequence 3 in a sequence table. Proved by experiments, the recombination blue-green alga disclosed by the invention is constructed through a metabolic engineering modification method, and carbon dioxide (CO2) can be directly converted into organic matter by utilizing solar energy by utilizing the characteristic that the blue-green alga is photoautotrophic; and the recombination blue-green alga can be directly synthetized into the optical pure D-lactic acid by utilizing the solar energy and the CO2 by utilizing the characteristic that the blue-green alga can grow in seawater and sewage and the advantage that genetic manipulation can be easily performed on the blue-green alga.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a lactic acid-producing recombinant blue-green algae and its preparation method and application. Background technique [0002] The energy problems and environmental problems facing the world have put forward the requirements for the development of renewable clean energy chemicals, and the food shortage requires the development of renewable clean energy chemicals to achieve the goal of not competing with people for food, crops, and People and crops compete for fresh water. [0003] Blue-green algae, also known as cyanobacteria, is a prokaryotic organism that can perform oxygen-evolving photosynthesis. Cyanobacteria are widely distributed in fresh water, sea water and even sewage, and can utilize CO 2 It reproduces rapidly with solar energy and is an ideal host for biosynthetic energy products. As a prokaryote, cyanobacteria have a simple cell structure and a similar genetic backgro...

AI Technical Summary

Problems solved by technology

In order to alleviate energy shortage and avoid environmental pollution, in recent years, researchers have attempted to transform bacteria through metabolic engineering to produce optically pure lactic acid by fermentation. Although this method avoids the problem of exacerbating the shortage of fossil energy, it will intensify the problem of food as raw material, such as mass production. The global food shortage problem cannot realize the sustainable development of renewable energy, so the use of solar energy directly converts CO 2 Photoautotrophic bacteria cyanobacteria converted to organic matter are an ideal pathway for the production of optically pure lactic acid

Images