Specific primers for identifying Tylopilus microsporus and identification method

Abstract

The invention relates to a pair of specific primers for identifying Tylopilus microsporus (T. microsporus) and an identification method. The specific primers are TMf and TMr, the DNA sequences of which are: TMf: GTCTTTCKCCATCCCAACCAACG; TMr: GAATCCAAACMACTCCAGCCCCG. The toxic and unconsciousness causing Tylopilus microsporus mixed in hybrid bolete samples can be identified by the following steps of: DNA extraction of boletus samples, PCR amplification by specific primers TMf and TMr taking the DNA as a template of the PCR amplification, electrophoresis of PCR amplification products in an agarose gel with concentration being 1%, ethidium bromide dyeing, amplification fragments detection by an ultraviolet transilluminator. The method provided by the invention has characteristics of high speed and high efficiency. The time from getting boletus mixed samples to obtaining identification results is only 2-3 hours.

Description

technical field [0001] The present invention relates to a kind of microsporum pollenia boletus ( Tylopilus microsporus S.Z.Fu, Q.B.Wang & Y.J.Yao) specific primers and a PCR identification method thereof, belonging to the technical field of identification of boletus with unclear food poisoning by means of molecular biology. [0002] Background technique [0003] Yunnan's special three-dimensional climate, complex soil structure, rich plant species and diverse vegetation types have bred a rich diversity of wild fungus resources, among which boletus is the most diverse wild fungus resource, and these resources are increasing in mountainous areas. It plays an important role in the income of farmers' families. In some remote areas, the income of wild mushrooms accounts for about 1 / 3-1 / 2 of the family's annual income. [0004] Among wild fungus resources, boletus resources are the most widely distributed, most diverse, highly morphologically similar, relatively difficult to id...

AI Technical Summary

Problems solved by technology

Accurate identification of the unclear poisonous Boletus microsporum boletus from mixed commercial boletus requires a certain basis of large-scale fungal taxonomy, but consumers often do not have this knowledge. Therefore, on the one hand, it leads to poisoning The occurrence of incidents, on the other hand, connived at unscrupulous dealers

At present, domestic and foreign researches on Pyrosporium boletus mainly focus on morphological classification and systematic research, and there are no reports on the rapid identification or detection of this bacterium using its specific primers

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