30results about How to "Protect food safety" patented technology

The invention relates to a pair of specific primers for identifying Tylopilus microsporus (T. microsporus) and an identification method. The specific primers are TMf and TMr, the DNA sequences of which are: TMf: GTCTTTCKCCATCCCAACCAACG; TMr: GAATCCAAACMACTCCAGCCCCG. The toxic and unconsciousness causing Tylopilus microsporus mixed in hybrid bolete samples can be identified by the following steps of: DNA extraction of boletus samples, PCR amplification by specific primers TMf and TMr taking the DNA as a template of the PCR amplification, electrophoresis of PCR amplification products in an agarose gel with concentration being 1%, ethidium bromide dyeing, amplification fragments detection by an ultraviolet transilluminator. The method provided by the invention has characteristics of high speed and high efficiency. The time from getting boletus mixed samples to obtaining identification results is only 2-3 hours.

The invention relates to an intelligent temperature control safety feeding bottle. The intelligent temperature control safety feeding bottle comprises a bottle body, a bottle neck and a suction nozzle; a bearing plate is arranged on the upper end of the bottle neck, and the bearing plate is combined with the bottle neck through screw threads; the lower surface of the bearing plate is provided with a temperature control layer; the temperature control layer expands with heat and contracts with cold; elastic current limiting plates are arranged below the temperature control layer, and the current limiting plates are made of a heat conductive material; the current limiting plates are arranged in rotational symmetry on the inner wall of the bottle neck, diversion holes are formed in the current limiting plates, and when the current limiting plates are perpendicular to the bottle neck, the current limiting plates are stacked in parallel in the vertical direction, meanwhile the diversion holes in the current limiting plates are communicated in a right-facing mode; when the current limiting plates are bent upwards or downwards, the diversion holes are staggered instead of being communicated; under the natural condition, the current limiting plates are arched upwards, and the diversion holes are staggered. The feeding bottle is novel and unique in structure, and can effectively guarantee the appropriate temperature of the drink, prevent from scalds and excessive coldness, give a baby the most intimate care, meanwhile relieve the burden of parents.

The invention discloses a tebuconazole pesticide-degrading bacterium, a soil bioremediation bacterium based on the bacterium and application thereof, belonging to the field of biotechnology. Its taxonomic name is Serratia marcescens H2 (Serratia marcescens H2). This strain is a Gram stain negative bacterium, and it is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 2016478 , date of deposit September 13, 2016. The Tebuconazole Pesticide Residue Degrading Bacteria is a strain with strong degrading ability to Tebuconazole isolated from the soil where Tebuconazole has been used for many years through enrichment culture. The strain grows with Tebuconazole as the only carbon source. In the liquid medium, the activity of the bacteria is increased through the induction of tebuconazole, and the degradation ability of the tebuconazole is improved. The preparation method of the bacterial agent is simple, the cost is low, and the application is convenient. It can be scattered on the soil, and the degradation effect in the soil can reach 88%. important meaning.

The invention relates to metribuzin pesticide residue-degrading bacteria; the degrading bacteria are named as bacillus cereus N1 (Bacillus cereus N1) by classification, and are Gram-positive bacteria; a preservation number of the degrading bacteria in China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2011163. Soil bioremediation microorganism prepared by the degrading bacteria and application are also disclosed. The metribuzin pesticide residue-degrading bacteria are bacterial strains which are obtained from sludge in a water spout of a insecticide factory for producing metribuzin and have strong degradation capacity to the metribuzin; the bacterial strains are grown by utilizing the metribuzin as exclusive nitrogen source; and thallus activity is increased by metribuzin induction in a liquid culture medium so as to improve the degradation capacity to the metribuzin. The microorganism provided by the invention is characterized by simple preparation method, relatively low cost and convenient implementation and can be dispersed on soil; degradation effect in the soil can reach to 68.19%; therefore, the microorganism has good application prospect.

The invention discloses a real-time florescent PCR (polymerase chain reaction) detection primer of fish composition in food, a kit and a detection method. The method comprises the following steps of: designing a primer for detection of fish genes and a probe SEQ ID (sequence identification) NO.1-3 aiming at the specific gene sequence of fish; and detecting fish composition in food quantitatively by adopting a real-time florescent PCR method. The real-time florescent PCR method quantitative detection method of fish composition in researched food has great significance for improving the detection efficiency and flexibility of fish composition in food, lowering the detection cost, detecting relevant false products in the market, monitoring food safety, and improving detection technique, and has wide development prospect.

The invention discloses a fumigation composition and an application thereof to the prevention and control of pests of volvaria volvacea. The fumigation composition for preventing and controlling the pests of the volvaria volvacea comprises the components in parts by volume: 1-2 parts of myrcene and 1 part of alpha-pinene; and the fumigation composition has prominent fumigation activity for the pests of the volvaria volvacea in an enclosed environment and is capable of preventing and relieving damage of the pests to mycelia of the volvaria volvacea. The fumigation composition disclosed by the invention belongs to a botanical insecticide and is easy to decompose, incapable of polluting the environment and safe to people and livestock; and the fumigation composition loses activity after deinsectization and cannot be accumulated in bodies of the pests or foods, so that the ecological environment and food safety are protected; and the fumigation composition is simple and feasible in preparation method, wide in raw material source, low in cost and suitable for large-scale production so as to be widely used for preventing and controlling the pests of the volvaria volvacea.

The invention discloses a real-time polymerase chain reaction (PCR) detection primer, a kit and a detection method for detecting puffer fish ingredients. The puffer fish gene detection primer and probes with the sequences of SEQ ID NO. 1-3 are designed according to the specific gene sequence of puffer fish, and the real-time fluorescent PCR method is adopted to qualitatively detect the puffer fish ingredients of food. The developed real-time fluorescent PCR qualitative detection method for detecting the puffer fish ingredients in food has importance significance in improving the detection efficiency and sensitivity of the puffer fish ingredients in food, lowering the detection cost, detecting related fake products on the market, monitoring food safety and improving a detection technology, and has wide development prospect.

The invention provides a special braided copper alloy wire for fishing nets for marine enclosure culture and a preparation method thereof. The wire comprises the following chemical components by mass percent: 58-75% of Cu, 0.4-1.8% of Sn, 0.2-0.5% of Si, 0.2-0.5% of Bi, not more than 0.004% of Pb, not more than 0.003% of Fe, not more than 0.0005% of Cd and the balance of Zn. The wire and the preparation method have the obvious advantages that the material is resistant to long-term seawater corrosion, is easy to braid and withstands force 25 hurricane blast; by adopting the process of replacing pulling with extruding, the blanks with high piece weights are obtained and the yield is increased; in addition, the production cycle is shortened, so that the wire is beneficial for popularization and application.

The invention discloses lactobacillus ingluviei for efficiently inhibiting avian pathogenic Escherichia coli and an application of the lactobacillus ingluviei. The lactobacillus ingluviei is separated from healthy chicken flocks and is named lactobacillus ingluviei SCGZ-9 with the preservation number of CCTCC NO: M2015337. The lactobacillus ingluviei SCGZ-9 has good acid resistance and cholate resistance and can effectively adhere to intestinal cells and inhibit adhesion of the avian pathogenic Escherichia coli to the cells. A supernatant secreted by the lactobacillus ingluviei SCGZ-9 can inhibit growth and propagation of the avian pathogenic Escherichia coli. Thus, the strain can be applied to preparation of preparations for preventing and / or treating the avian pathogenic Escherichia coli or preparation of preparations for improving ecological balance of intestinal flora of birds.

The invention relates to a leek maggot killing agent. The leek maggot killing agent comprise the following effective components in percentage by weight: 6-8 parts of sulphur, 1-2 parts of a high-efficiency dispersing agent, and 1-2 parts of a synergist. The leek maggot killing agent is prepared by taking the sulphur as a base component, adding the high-efficiency dispersing agent and the synergist and mixing, and crushing into 1800-2600-mesh superfine powder by using an airflow crushing device. The leek maggot killing agent is simple in production process and stable in product quality; after the superfine powder is mixed with water, the dispersibility is excellent and the suspensibility is high and a uniform and stable suspension is formed in water rapidly; the suspension permeates into the ground surface to contact a leek maggot body and corrode the leek maggot body so as to solidify proteins and block pores of the leek maggot body, a leek maggot is further killed; the leek maggot killing agent is lasting in effect, low in cost, safe, non-toxic, pollution-free and residue-free, not only reduces the burden of a leek farmer, but also has an important significance for environment protection and food safety.