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Metribuzin-degrading bacteria and soil bioremediation microorganism based on metribuzin-degrading bacteria and application

A technology of soil biology and restoration of bacterial agents, applied in the biological field, can solve the problems of sensitive crops that are prone to phytotoxicity, affect the growth of higher plants, and have a long residual time, so as to protect the ecological environment and food safety, eliminate pesticide residues, and be easy to use Effect

Inactive Publication Date: 2013-04-17
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of herbicide has the advantages of fast weeding speed and low toxicity to humans and animals. However, due to long-term use or improper use, it is easy to cause phytotoxicity to sensitive crops, which not only affects the growth of higher plants, but also affects the surface, groundwater, soil and animals. certain hazard
Some of them, such as mecitrione, remain in the soil for a long time, and are prone to phytotoxicity to crops such as sugar beets and vegetables after cropping.

Method used

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  • Metribuzin-degrading bacteria and soil bioremediation microorganism based on metribuzin-degrading bacteria and application
  • Metribuzin-degrading bacteria and soil bioremediation microorganism based on metribuzin-degrading bacteria and application
  • Metribuzin-degrading bacteria and soil bioremediation microorganism based on metribuzin-degrading bacteria and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Enrichment, separation, purification and preparation of bacterial suspension of mecitrione-degrading bacteria

[0027] Get 5g of soil samples (Jilin Agricultural University Agricultural Science Experimental Station has used metrimetrione test field for many years) and add it into a triangular flask filled with 100mL inorganic salt medium, and take 2mL from the prepared metrimetrione solution with a concentration of 1000mg / L. Put it into the Erlenmeyer flask as the only nitrogen source (20mg / L) for microbial growth, culture on a shaker for 2d (30°C, 160r / min) and then transfer to the inorganic salt medium containing mecitrione 50mg / L for 2d. , and then insert 0.1 mL of the above-mentioned inorganic salt culture medium for 2 days on the plate culture medium containing metrimetrione with a concentration of 100 mg / L, and culture it in a 30°C incubator for 2 days after coating, and pick out a single colony on the plate Repeated transplantation and streaking for 3 ...

Embodiment 2

[0030] Embodiment 2: degradation condition

[0031] 1. The effect of pH on the degradation of mecitrione by strain N1

[0032] According to the percentage by volume, the inoculation amount is 5%, and the N1 bacterial suspension prepared in Example 1 is inserted into the inorganic salt medium whose concentration of mecitrione is 20mg / L, and the pH value of the medium is adjusted to 5.0, 6.0, 6.5 respectively. , 7, 7.5, 8.0, 9.0, cultured on a shaking table at 30°C and 160rpm, and measured the content of metrizone at 24, 48, 72, 96, and 120 hours respectively.

[0033] The formula of the inorganic salt culture medium is by weight percentage: sucrose 3g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.4g, K 2 HPO 4 1.5g, 1g NaCl, add 1000mL distilled water, the pH is natural.

[0034] The experimental results of the pH effect on the degradation of metrizone by the strain showed that when the pH was 7.0, the degradation rate reached 78.43% within 120 hours, and the degradation efficiency ...

Embodiment 3

[0050] Embodiment 3: Bacteria agent preparation test

[0051] Use an inoculation loop to inoculate a little bacteria on the plate culture medium in the slant test tube strain of mecitrione-degrading bacteria N1, incubate at 30°C for 48 hours, then use an inoculation loop to inoculate a little bacteria in LB medium, 30°C Shake culture to logarithmic phase.

[0052] The formula of described plate culture medium is by weight percentage: NaCl 5g, peptone 10g, beef extract 5g, agar 15g, NaHPO 4 1.50g, add 1000mL distilled water; the LB medium is: NaCl 10g, yeast powder 5g, tryptone 5g, add 1000mL distilled water, the pH is 7.0; put 10mL of the cultured strain into the seed bottle, shake and cultivate to logarithmic period; 1000mL seed bottle medium is: sucrose 5g, MgSO 4 ·7H 2 O 0.8g, K 2 HPO 4 2g, NaCl 1g, KH 2 PO 4 0.5g, 0.2g yeast extract, add 1000mL water, the pH of the medium is 7.0;

[0053] Add 15 mL of the above-mentioned cultured strains to 1000 g of fermentatio...

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Abstract

The invention relates to metribuzin pesticide residue-degrading bacteria; the degrading bacteria are named as bacillus cereus N1 (Bacillus cereus N1) by classification, and are Gram-positive bacteria; a preservation number of the degrading bacteria in China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2011163. Soil bioremediation microorganism prepared by the degrading bacteria and application are also disclosed. The metribuzin pesticide residue-degrading bacteria are bacterial strains which are obtained from sludge in a water spout of a insecticide factory for producing metribuzin and have strong degradation capacity to the metribuzin; the bacterial strains are grown by utilizing the metribuzin as exclusive nitrogen source; and thallus activity is increased by metribuzin induction in a liquid culture medium so as to improve the degradation capacity to the metribuzin. The microorganism provided by the invention is characterized by simple preparation method, relatively low cost and convenient implementation and can be dispersed on soil; degradation effect in the soil can reach to 68.19%; therefore, the microorganism has good application prospect.

Description

technical field [0001] The invention belongs to the field of biological technology, and specifically relates to a metrizone pesticide-degrading bacterium and a soil bioremediation bacterial agent prepared based on the bacterium, which uses microbial technology to degrade chemical pesticide residues, and is suitable for green and pollution-free agricultural products in modern agricultural production production and processing. Background technique [0002] Mecitrione is a triazine herbicide developed by German Bayer in 1971, mainly used in soybean fields to control broad-leaved and gramineous weeds. This type of herbicide has the advantages of fast weeding speed and low toxicity to humans and animals. However, due to long-term use or improper use, it is easy to cause phytotoxicity to sensitive crops, which not only affects the growth of higher plants, but also affects the surface, groundwater, soil and animals. Certain hazards. Some of them, such as azimidone, remain in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/085
Inventor 张浩高鹤南侯志广赵晓峰逯忠斌王岩
Owner JILIN AGRICULTURAL UNIV
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