Lactobacillus ingluviei for efficiently inhibiting avian pathogenic Escherichia coli and application of lactobacillus ingluviei

A Lactobacillus and Escherichia coli technology, which is applied to medical preparations containing active ingredients, bacteria, antibacterial drugs, etc., can solve the problems of economic harm to the poultry industry, death of chicks, and rough feathers, etc., to protect food safety and Human health, inhibition of adhesion, good stability

Active Publication Date: 2015-11-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sick chickens infected by pathogenic Escherichia coli are depressed, have loss of appetite, rough feathers, and emaciation, which can cause symptoms such as acute sepsis, diarrhea, air sacculitis, peritonitis, etc., and can also cause chickens after being spread by feces-contaminated chicken embryos. A large number of deaths, so colibacillosis causes great harm and serious economic losses to the poultry industry

Method used

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  • Lactobacillus ingluviei for efficiently inhibiting avian pathogenic Escherichia coli and application of lactobacillus ingluviei
  • Lactobacillus ingluviei for efficiently inhibiting avian pathogenic Escherichia coli and application of lactobacillus ingluviei
  • Lactobacillus ingluviei for efficiently inhibiting avian pathogenic Escherichia coli and application of lactobacillus ingluviei

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. The source of Lactobacillus SCGZ-9

[0027] The Lactobacillus was isolated by the inventor from healthy chickens in a Guangdong farm in mid-2013, and is now preserved and provided by the Poultry Disease Research Office of the Veterinary College of South China Agricultural University.

[0028] 2. Identification and classification of Lactobacillus SCGZ-9

[0029] (1) Biochemical identification

[0030] According to the "Berger's Bacterial Identification Manual", do the following biochemical tests: catalase test, nitrate reduction test, gelatin liquefaction test, indole test, hydrogen sulfide test, V-P test and dynamic test. The seven biochemical tests were all negative. It was determined that the screened SCGZ-9 strain belonged to the genus Lactobacillus.

[0031] (2) 16SrRNA gene sequencing

[0032] Design primer sequence: fD1: 5'-AGAGTTTGATCCTGGCTCAG-3', rD1: 5'-AAGGAGGTGATCCAGCC-3', PCR reaction was carried out according to the instruction manual of rTaq DNA pol...

Embodiment 2

[0044] 1. In vivo environmental tolerance test

[0045] The digestive tract of the body is a complex environment, especially the stomach and intestinal tract. The environment in the stomach of chickens is strongly acidic, which can kill most of the microorganisms that enter the stomach, while there are bile and various secretions in the small intestine, which can inhibit microbial growth. For probiotics to enter the intestinal tract and play a role, in addition to providing protection through the production process strains, the strains themselves should also have a certain tolerance to environments such as low pH and the presence of bile salts. Many current studies use the method of simulating the gastrointestinal environment in vitro to evaluate the tolerance of probiotics to the in vivo environment.

[0046] (1) Acid resistance test

[0047] The SCGZ-9 strain was passaged three times in MRS broth, and the concentration was adjusted at 10 8 About CFU / mL, inoculate 5% (v / v)...

Embodiment 3

[0055] Example 3: Inhibitory effect of Lactobacillus SCGZ-9 on pathogenic Escherichia coli in vivo

[0056] 1. Agar Diffusion Test

[0057] The SCGZ-9 strain was inoculated in MRS broth, recovered at 37°C, subcultured twice after 24 hours, inoculated in MRS broth, centrifuged at 8000r / min for 20min, and the supernatant was taken, which was the suspension of Lactobacillus SCGZ-9. liquid.

[0058] Pathogenic Escherichia coli (Enteropathogenic Escherichiacoli, EPEC) (chicken pathogenic Escherichia coli O78 , serotype O78: K80, number GIM1.739, purchased from Guangdong Microbial Culture Collection Center) densely spread on LB nutrient agar plate (ingredients (g / L): tryptone 10.0, yeast extract powder 5.0, sodium chloride 10.0, agar 15.0, pH value 7.0±0.2), make chicken pathogenic Escherichia coli O 78 Cover the plate evenly and place it at 37°C for 20min. Take out the flat plate in the ultra-clean bench, and punch three holes vertically on the flat plate with a sterile puncher ...

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Abstract

The invention discloses lactobacillus ingluviei for efficiently inhibiting avian pathogenic Escherichia coli and an application of the lactobacillus ingluviei. The lactobacillus ingluviei is separated from healthy chicken flocks and is named lactobacillus ingluviei SCGZ-9 with the preservation number of CCTCC NO: M2015337. The lactobacillus ingluviei SCGZ-9 has good acid resistance and cholate resistance and can effectively adhere to intestinal cells and inhibit adhesion of the avian pathogenic Escherichia coli to the cells. A supernatant secreted by the lactobacillus ingluviei SCGZ-9 can inhibit growth and propagation of the avian pathogenic Escherichia coli. Thus, the strain can be applied to preparation of preparations for preventing and / or treating the avian pathogenic Escherichia coli or preparation of preparations for improving ecological balance of intestinal flora of birds.

Description

technical field [0001] The invention relates to a new bacterial strain and its application in biological control technology, in particular to a lactobacillus capable of efficiently inhibiting pathogenic Escherichia coli from avian origin and its application. Background technique [0002] Probiotics are a class of living microorganisms that can be directly used as food additives and benefit the health of the host. Lactobacillus is one of the most commonly used probiotics. If animals consume sufficient amounts of lactic acid bacteria for a long time, lactic acid bacteria can stably colonize the intestinal tract of animals, improve the ecological balance of intestinal microorganisms and inhibit the invasion of pathogenic bacteria, and protect animals from diseases. , At the same time, some lactic acid bacteria also have the effect of promoting digestion and increasing animal weight gain. Lactic acid bacteria have been widely used in human food. In recent years, lactic acid bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04C12R1/225
Inventor 罗开健张宗尧廖明张建民王媚吕嘉敏黄玉梅
Owner SOUTH CHINA AGRI UNIV
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