Poultry egg windowing and sealing method

A poultry egg and window opening technology, which is applied in the field of poultry egg window opening and sealing, can solve problems such as difficult unification of standards, hatching failure, and low success rate of operation, and achieve the effects of guaranteed operation time, easy control of the process, and high hatching rate

Inactive Publication Date: 2012-08-01
刘红林 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there are also some obvious problems in this method: as, when dripping liquid to cover the opening, the amount of dripping liquid is not easy to control, and if there is too much dripping, it will easily flow and cause the failure of sealing air, and if the dripping is less, it will lose the effect of blocking air entry (and most Eggs will absorb more liquid), and the shape of different eggs varies greatly, so it is difficult to have a unified standard; moreover, some eggshell surfaces are often hydrophilic, and it is impossible to form droplets to achieve the purpose of sealing. Applying hydrophobic substances such as paraffin oil on the surrounding area has a certain effect, but i

Method used

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  • Poultry egg windowing and sealing method
  • Poultry egg windowing and sealing method
  • Poultry egg windowing and sealing method

Examples

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Effect test

Embodiment 1

[0024] 1.1 Opening windows of poultry eggs: Fertilized eggs are taken from Taicang breeder farm of Wen's Group. The fresh fertilized eggs are sterilized by 75% alcohol and placed flat on the egg plate. After the highest point is marked with ordinary ink, the germinal disc is fixed overnight at room temperature. The next day, use a mini electric drill (Taiwan Baogong, model 1PK-500B-2) to polish a window of about 0.3-0.6 cm square at the mark on the side of the egg, while paying attention to maintaining the integrity of the eggshell membrane. Use a soft brush to clean the shell powder on the surface first, and then wipe it clean with 75% alcohol cotton balls. After drying, use a hot melt glue gun (Joel Thunder, model XL-E20K, ordinary EVA hot melt adhesive) to push the glue to make the hot melt The glue forms a concentric closed circle with the center of the window as the center and an inner diameter of about 1 cm. The hot-melt glue is solidified, and the concentric closed cir...

Embodiment 2

[0038] The fertilized eggs were taken from the Taicang breeder farm of Wen's Group. The fresh fertilized eggs are sterilized by 75% alcohol and placed flat on the egg plate. After the highest point is marked with ordinary ink, the germinal disc is fixed overnight at room temperature. The next day, use a mini electric drill (Taiwan Baogong, model 1PK-500B-2) to polish a window of about 0.3-0.6 cm square at the mark on the side of the egg, while paying attention to maintaining the integrity of the eggshell membrane. Use a soft brush to clean the shell powder on the surface, and then wipe it with 75% alcohol cotton balls. After drying, use gelatin to form a concentric closed circle with the center of the window as the center and an inner diameter of about 1 cm on the eggshell. After solidification, drop double-antibody (1‰w / v ampicillin and 1‰w / v streptomycin, the same below) and 2μg / ml PBS (pH7.2, 0.02M) in the gelatin ring to full , carefully cut the eggshell membrane along th...

Embodiment 3

[0040] The method is the same as in Example 2, only the gelatin is replaced by hot melt glue, the Vc concentration is changed to 20 μg / ml, the hatching rate reaches 45.2%, and the transgene detection efficiency is 48% in the effectively extracted samples.

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Abstract

The invention discloses a poultry egg windowing and sealing method. The invention comprises the steps: windowing on a poultry egg equatorial plate position, keeping a full eggshell membrane, forming a concentric sealing ring on the eggshell by using hot melt adhesive or the like, taking a window center as a circle center, dropwise adding physiological buffer solution after the concentric sealing ring and the eggshell surface are bonded firmly, removing the eggshell membrane under the buffer solution blockage condition, positioning an embryo and performing the embryo operation, then fully covering the window under the solution by using the prepared eggshell membrane, absorbing surplus solution in the sealing ring by using absorbent paper, then filling the interior of the sealing ring by using the hot melt adhesive or gelatin. The poultry egg windowing and sealing method effectively increases transgenic poultry egg hatching rate and transgenic efficiency by optimizing an operation proposal.

Description

technical field [0001] The invention belongs to the field of transgenic technology, and relates to a method for opening and sealing poultry eggs. Background technique [0002] Poultry transgenic is of great significance for obtaining animal models of specific genotypes and producing therapeutically valuable medicinal proteins, and has huge scientific and commercial potential value. Due to the special reproductive and physiological characteristics of poultry and the limitation of technical conditions, the most commonly used and most practical method is to conduct genetically modified operations on poultry eggs. Manipulation of poultry eggs necessarily involves opening the shell and shell membrane to gain access to the embryo. It can be divided into two cases. One is the method of changing egg shells, that is, the manipulated poultry embryo egg is relatively small, and the egg shell is opened at the blunt end. For example, after transgenic operation with chicken embryos, mov...

Claims

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Application Information

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IPC IPC(8): A01K45/00
Inventor 徐世永翟乐张宏晓陈维康戴鼎震王宪叶刘红林
Owner 刘红林
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