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Method for quickly detecting sensibility of two-spotted spider mite to avermectin

A technology for abamectin and Tetranychus 2-spotted mites is applied in the field of rapid detection of susceptibility of Tetranychus urticae to abamectin, and can solve the problems of low pesticide concentration, low orifice concentration, inaccurate determination of mortality, and the like, Achieving the effect of suitable pesticide concentration and improving reliability

Inactive Publication Date: 2012-08-01
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the resistance monitoring of Tetranychus urticae, which are small in size and easy to form nets, mostly adopts the slide dipping method or the centrifuge tube method. The slide dipping method needs to be treated for 24 hours before the result can be obtained, and it is difficult to achieve the purpose of rapid detection. , and the glass slide dipping method provides an open space for the two-spotted spider mite, and the pesticide concentration in the effective space is too low; the centrifugal tube method is due to the high concentration at the bottom of the tube and the low concentration at the mouth of the tube, which leads to inaccurate mortality determination. Therefore, it is urgently needed Find a method that can quickly and accurately detect the sensitivity of spider mite to abamectin

Method used

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  • Method for quickly detecting sensibility of two-spotted spider mite to avermectin
  • Method for quickly detecting sensibility of two-spotted spider mite to avermectin
  • Method for quickly detecting sensibility of two-spotted spider mite to avermectin

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Embodiment 1

[0021] A method for rapidly detecting the sensitivity of Tetranychus urticae to abamectin, the concrete steps are:

[0022] (1) Pick female adult mites that are uniform in size, bright in body color, and active in action, and fix them neatly on one end of a glass slide, sticking 30 heads on each slide;

[0023] (2) After placing the above-mentioned mite-bearing slides in an artificial climate box for 1 hour, observe with binoculars, remove dead and inactive individuals, and keep 20 healthy individuals;

[0024] (3) Dilute the abamectin to 0.1 mg / L, immerse the slide with mite in the liquid medicine, shake it gently, take it out after 5 seconds, and quickly absorb the excess liquid medicine around the mite body and surrounding area with absorbent paper;

[0025] (4) Put the slides with mites into the petri dish, seal them with a parafilm to form an airtight drug film space, place them in an artificial climate box for 1 hour, check the mortality rate, and calculate the corrected...

Embodiment 2

[0027] A method for rapidly detecting the sensitivity of Tetranychus urticae to abamectin, the concrete steps are:

[0028] (1) Pick female adult mites with the same size, bright body color and lively action, and fix them neatly on one end of the slide, sticking 40 heads on each slide;

[0029] (2) After placing the above-mentioned mite-bearing slides in an artificial climate box for 1 hour, observe with binoculars, remove dead and inactive individuals, and keep 30 healthy individuals;

[0030] (3) Dilute the abamectin to 0.2 mg / L, immerse the mite-carrying glass slide in the liquid medicine, shake it gently, take it out after 5 seconds, and quickly absorb the excess liquid medicine around the mite body and surrounding area with absorbent paper;

[0031] (4) Put the slides with mites into the petri dish, seal them with a parafilm to form an airtight drug film space, place them in an artificial climate box for 1 hour, check the mortality rate, and calculate the corrected mortal...

Embodiment 3

[0033] A method for rapidly detecting the sensitivity of Tetranychus urticae to abamectin, the concrete steps are:

[0034] (1) Pick female adult mites with uniform size, bright body color, and active actions, and fix them neatly on one end of a glass slide, sticking 50 heads on each slide;

[0035] (2) After placing the above-mentioned mite-bearing slides in an artificial climate box for 1 hour, observe with binoculars, remove dead and inactive individuals, and keep 40 healthy individuals;

[0036] (3) Dilute the abamectin to 0.4 mg / L, immerse the glass slide with mites in the liquid medicine, shake it gently, take it out after 5 seconds, and quickly dry the mites and the excess liquid around them with absorbent paper;

[0037] (4) Put the slides with mites into the petri dish, seal them with a parafilm to form an airtight drug film space, place them in an artificial climate box for 1 hour, check the mortality rate, and calculate the corrected mortality rate with reference to...

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Abstract

The invention relates to a method for quickly detecting the sensibility of a two-spotted spider mite to avermectin. A sealed and stable space is formed by mainly adopting a culture dish film sealing method; the concentration of a drug is enabled to be appropriate, uniform and stable; the drug effect of an agricultural pesticide is enabled to appear adequately within a shorter time; and the sensibility of the two-spotted spider mite to the avermectin can be quickly and accurately detected.

Description

technical field [0001] The invention relates to a method for rapidly detecting the sensitivity of the two-spotted spider mite to abamectin. Background technique [0002] Tetranychus urticae is an important worldwide pest. The mite is prone to resistance to chemical pesticides. Four years after the registration of Tetranychus urticae on Australian cotton, it developed resistance to pesticides. In the 1980s, Tetranychus urticae was introduced into my country. Because it had developed resistance to many kinds of pesticides before it was introduced into my country, the domestic control mainly relied on chemical pesticides, which made the resistance of Tetranychus urticae develop rapidly, causing damage getting worse. [0003] As an antibiotic insecticide and acaricide, avermectin works by stimulating the insect body to produce γ-aminobutyric acid, blocking the transmission of motor nerve information, and causing the insect to quickly paralyze, refuse to eat, slow down or stop wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 于毅马惠周仙红张安盛刘庆娟门兴元张思聪李丽莉韩凤英张毓妹张士云
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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