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ShRNA of Rab23 and ientiviral vector and applications thereof

A technology of gv248-rab23-shrna and carrier, applied in virus/bacteriophage, DNA/RNA fragments, applications, etc., can solve problems such as low transfection efficiency, easy degradation of siRNA fragments, and inability to realize RNA interference

Inactive Publication Date: 2012-08-22
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0003] In the prior art, some scholars directly transfect mammalian cells with chemically synthesized siRNA fragments, which can specifically inhibit the expression of intracellular homologous genes, but the transfection efficiency is low, and intracellular siRNA fragments are easily degraded, so this method Unable to achieve stable RNA interference

Method used

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  • ShRNA of Rab23 and ientiviral vector and applications thereof
  • ShRNA of Rab23 and ientiviral vector and applications thereof
  • ShRNA of Rab23 and ientiviral vector and applications thereof

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Embodiment Construction

[0028] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the present invention.

[0029] 1. The correlation between the expression of Rab23 protein and skin squamous cell carcinoma

[0030] Differences in the expression of Rab23 in normal skin, precancerous lesions of skin squamous cell carcinoma, actinic keratosis, squamous cell carcinoma in situ, and invasive squamous cell carcinoma tissues:

[0031] 31 cases of normal skin tissue (Normal) after surgical resection, 31 cases of actinic keratosis tissue (AK) of precancerous lesions of skin squamous cell carcinoma, 42 cases of in situ squamous cell carcinoma tissue (Bowen's), invasive squamous cell carcinoma tissue (SCC) ) 45 cases. Fixed in 15% formalin, embedded in paraffin and sectioned. Paraffin sections were dewaxed and rehydrated with xylene and graded ethanol, boiled in citrate buffer for antigen retriev...

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Abstract

The invention discloses an interference Rab23 gene of siRNA. The siRNA is hairpin RNA (ribonucleic acid) formed aiming to a target sequence of the Rab23 gene; the siRNA is double-strand siRNA, wherein one strand of nucleotide sequence is shown as SEQ. ID. NO.1 or SEQ. ID. NO.2; the expression of the Rab23 protein is positive correlation with the development of skin scale cancer and is especially related with invasion ability of the skin scale cancer; and the invasion ability of skin scale cancer cells is enabled to lowered by the expression of the interference Rab23 protein. The invention also discloses applications of interference shRNA of the Rab23 gene target sequence in preparing a skin scale cancer resisting drug.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of skin squamous cell carcinoma, and relates to a shRNA of Rab23 and its lentiviral vector and application. Background technique [0002] Rab23 is a member of the RAS proto-oncogene, and the protein encoded by it is a member of the small GTPase superfamily Rab family. Its mutation can cause open brain syndrome in mice, so it is also called opb gene. In 2001, Eggenschwiler et al. found that Rab23 is a necessary negative regulator of Sonic Hedgehog signaling pathway. In recent years, the role of Rab23 as a proto-oncogene has been paid more and more attention by scholars. Studies have found that Rab23 is involved in the occurrence and development of liver cancer, gastric cancer, lung cancer, breast cancer, and thyroid cancer, and was found to be closely related to tumor invasion in gastric cancer for the first time. [0003] In the prior art, some scholars directly transfect mammali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/867C12N7/01A61K48/00A61P35/00A61P35/04
Inventor 李承新简强唐利刘博强薛柯苗叶
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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