Standard fingerprint of fleabane phenol bulk drug and preparation, establishment method and application

A standard fingerprint, the technology of Dengzhan Asarum is applied to the standard fingerprint of Dengzhan Asarum raw materials and preparations, and its establishment, the standard fingerprint and its application in the quality control of raw materials and preparations can solve the problem. Problems such as low quality and low identification rate of Dengzhan asarol raw materials and preparations cannot be well realized

Active Publication Date: 2012-08-29
YUNNAN SHIPURUI BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the purpose of the present invention is to aim at the fingerprint chromatogram of breviscapine medicinal material and the identification rate of characteristic fingerprint peaks in the chromatogram is relatively low (67%) at present, and it is impossible to realize the transformation from breviscapine medicinal material to scutellarin. Insufficient technology and quality of raw materials and preparations, provide a fingerprint of scutellaria scutellaria medicinal material with an identification rate of 95%, and the establishment method of fingerprints of scutellarin raw materials and preparations and the obtained standard fingerprints, so as to Realize the whole-process monitoring of process and quality from scutellaria to scutellarin raw materials and preparations, facilitate the confirmation of scutellaria, scutellarin raw materials and basic substances of preparations, production process and quality assessment Evaluation, and provide standard indications for subsequent process improvement and perfection

Method used

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  • Standard fingerprint of fleabane phenol bulk drug and preparation, establishment method and application
  • Standard fingerprint of fleabane phenol bulk drug and preparation, establishment method and application
  • Standard fingerprint of fleabane phenol bulk drug and preparation, establishment method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Establishment of the standard fingerprint of Erigeron breviscapus medicinal material

[0035] 1. Chromatographic conditions and system applicability Agilent 1100 Series HPLC; Zorbax SB-C18 (4.6mm×250mm, 5μm) chromatographic column; acetonitrile-methanol-formic acid as mobile phase; detection wavelength: 270nm, 330nm; theory The plate number should not be less than 6000 based on the calculation of the scutellarin peak.

[0036] 2. Preparation of the reference substance solution Take an appropriate amount of the scutellarin reference substance and prepare it into a 0.4 mg / mL methanol solution.

[0037] 3. Preparation of the test solution Take 0.50 g of Erigeron breviscapus medicinal material, heat and reflux with methanol to extract, evaporate the extract to dryness, dissolve it with 60% methanol and dilute to 10 mL, filter through a 0.45 μm filter membrane, and obtain.

[0038] 4. Determination method Precisely draw 5 μL each of the reference substance solu...

Embodiment 2

[0049] Example 2: Establishment of standard fingerprint of scutellarin crude drug

[0050] 1. Chromatographic conditions and system applicability Agilent 1100 Series HPLC; Zorbax SB-C18 (4.6mm×250mm, 5μm) chromatographic column; acetonitrile-methanol-formic acid as mobile phase; detection wavelength: 270nm, 330nm; theory The plate number should not be less than 6000 based on the calculation of the scutellarin peak.

[0051] 2. Preparation of reference substance solution Take an appropriate amount of scutellarin reference substance and add methanol to make a 0.4 mg / mL solution.

[0052] 3. Preparation of the test product solution Precisely weigh an appropriate amount of the test product to prepare a 1 mg / mL methanol solution.

[0053] 4. Determination method Precisely draw 5 μL of the reference substance solution and the test solution, and inject them into the liquid chromatograph respectively for determination.

[0054] 5. Establishment of standard fingerprint of scutellar...

Embodiment 3

[0085] Example 3: Establishment of standard fingerprints of scutellarin preparations

[0086] 1. Chromatographic conditions and system applicability Agilent 1100 Series HPLC; Zorbax SB-C18 (4.6mm×250mm, 5μm) chromatographic column; acetonitrile-methanol-formic acid as mobile phase; detection wavelength: 270nm, 330nm; theory The plate number should not be less than 6000 based on the calculation of the scutellarin peak.

[0087] 2. Preparation of reference substance solution Take an appropriate amount of scutellarin reference substance and add methanol to make a 0.4 mg / mL solution.

[0088] 3. Preparation of the test product solution Precisely weigh an appropriate amount of the test product to prepare a 0.02 g / mL methanol solution.

[0089] 4. Determination method Accurately draw 5 μL each of the reference substance solution and the test solution, inject it into the high-performance liquid chromatograph, and measure it.

[0090] 5. Establishment of standard fingerprint of sc...

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Abstract

The invention provides a standard fingerprint of fleabane phenol bulk drugs and preparations, an establishment method and application. The technical schemes comprise the determination of the standard fingerprint and the characteristic fingerprint peak of fleabane drug materials, the fleabane phenol bulk drugs and the preparations, and the application of the standard fingerprint in the aspects of extraction and processing, and quality control in the process of producing the fleabane phenol bulk drugs and the preparations. Therefore, the overall monitoring of the process and the quality from the fleabane drug materials to fleabane phenol bulk drugs and then the preparations can be realized, basic substances of the fleabane drug materials, the fleabane phenol bulk drugs and the preparations can be conveniently confirmed, and the production process and quality can be conveniently evaluated, and standard indications can be provided for subsequent process improvement and perfection.

Description

technical field [0001] The present invention relates to a method for establishing standard fingerprints of traditional Chinese medicine from medicinal materials to raw materials and then to preparations, in particular to a standard fingerprint of scutellarin raw materials and preparations, its establishment method, and the standard fingerprint obtained by the method Atlas and its application in the quality control of raw materials and preparation processes. Background technique [0002] Traditional Chinese medicine fingerprint refers to the chromatogram or spectrum of a certain type or several types of components that are common in a certain Chinese medicinal material or its raw materials and preparations. At the present stage, most of the active ingredients of traditional Chinese medicine are not very clear, the establishment of fingerprints is of great significance for the effective control of the quality of Chinese medicinal materials and their raw materials and preparati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 孙汉董罗晓星王海燕龙祥胡志祥赵勤实
Owner YUNNAN SHIPURUI BIOLOGICAL ENG
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