Method for evaluation of strain quality of trichoderma biocontrol agent

Abstract

The invention relates to a method for evaluation of strain quality of a trichoderma biocontrol agent. The method comprises the following steps of evaluation of growth characteristics of the trichoderma biocontrol agent, evaluation of antagonism of the trichoderma biocontrol agent, evaluation of a solid-fermentation spore-production amount of the trichoderma biocontrol agent, evaluation of preparation storability and a spore survival rate of the trichoderma biocontrol agent, evaluation of a colonization survival amount and biocontrol effects of the trichoderma biocontrol agent under pot experiment conditions, and evaluation of a colonization survival amount, biocontrol effects and ecological efficiency of the trichoderma biocontrol agent under field application conditions. The method is conducive to screening of novel trichoderma strains having good biocontrol effects and environmentally-friendly characteristics, can increase a strain screening rate, and provides an effective approach for fast search of novel trichoderma strains having economic values.

Description

technical field [0001] The invention relates to a germplasm evaluation method of Trichoderma biocontrol bacteria, which belongs to the technical field of agricultural biological control. Background technique [0002] Biological control is one of the important contents of the integrated control of crop diseases and insect pests. Biopesticide is the most widely used measure in biological control and has the largest application area. The application of biopesticide in my country has a good foundation, and it has developed rapidly from the 1980s to the 1990s , has played an important role in the integrated control of plant diseases and insect pests in rice, wheat, cotton, corn, fruit trees, vegetables and other crops. Although there is a period of trough, at present, people pay more and more attention to environmental protection, ecological harmony and sustainable development, and put forward the concept of "public plant protection, green plant protection", and biopesticides are ...

AI Technical Summary

Problems solved by technology

[0003] Trichoderma biocontrol fungicides are living microbial fungicides, and their innovation and utilization process is complex, with many bactericidal mechanisms and environmental factors, and there are many additional conditions involved in promotion, which determines the evaluation of Trichoderma biocontrol germplasm innovation. It should be a systematic project, involving the comparison of plate growth characteristics, screening of antagonistic effects, improvement of production process and spore production, storage period of preparations and spore survival rate, and colonization survival of Trichoderma biocontrol bacteria under pot test and field application conditions. And a series of indicators such as survival time and biological control effect. At present, it is difficult to meet the requirements by using the same single efficacy evaluation standard as that of chemical pesticides. It only involves one part of it, which is not comprehensive. As an evaluation indicator for the safety of farmland ecosystems, it is difficult to highlight the advantages of biological pesticides on the safety of the ecological environment and the safety of agricultural products.

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