Capillary electrophoresis-chemiluminescence detection interface device and preparation method thereof

Abstract

The invention relates to a capillary electrophoresis-chemiluminescence detection interface device and a preparation method thereof. The interface device comprises organic glass, a reaction capillary and an isolation capillary, a transverse through channel and a longitudinal luminescence detecting window are arranged in the middle of the organic glass, the reaction capillary is arranged in the transverse through channel, at least two positioning gaskets are arranged between the outer wall of the reaction capillary and the inner wall of the transverse through channel, one end of the isolation capillary is inserted into a feeding end of the reaction capillary while the other end of the isolation capillary penetrates through a rubber plug which is mounted on a corresponding end port of the transverse through channel, and a luminescence reagent channel and an electrode mounting channel are further arranged on two radial sides of the isolation capillary. The capillary electrophoresis-chemiluminescence detection interface device is low in preparation cost, an interface is small and exquisite, and the device is easy and convenient to operate, can effectively solve the problems of lowering of isolation efficiency, sensitivity and stability and the like due to introduction of CL (chemiluminescence) reagent, has high CE (capillary electrophoresis) isolation efficiency and high CL detection sensitivity and can be used for analysis of biological samples, food and environmental samples.

Description

technical field [0001] The invention relates to a capillary electrophoresis-chemiluminescence detection interface device and a manufacturing method thereof, which are suitable for analyzing biological samples, food and environmental samples. Background technique [0002] Capillary electrophoresis (capillary electrophoresis, CE) has the characteristics of high efficiency, rapidity, small injection volume, wide separation range, low operating cost, low solvent consumption, simple operation and strong anti-pollution ability, especially suitable for complex sample analysis. One of the most dynamic frontiers of research. However, commercial CE instruments mostly use ultraviolet detectors (UV), and the minimum detection limit is generally 10 -5 ~10 -6 mol / L, it is difficult to meet the requirements of extremely low content component analysis in complex samples, which limits the application of CE in the field of complex sample analysis research. [0003] Chemiluminescence (CL) d...

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Problems solved by technology

[0011] The purpose of the present invention is to provide a capillary electrophoresis-chemiluminescence detection interface device and its manufacturing method. The interface device has low manufacturing cost, small interface, easy operation, and better solves the separation efficiency, sensitivity and problems caused by the introduction of CL reagent. Problems such as reduced stability, with high separation efficiency of CE and high sensitivity of CL detection

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